Characterization and anti-inflammation role of swine IFITM3 gene

Abstract

IFITM3 is involved in cell adhesion, apoptosis, immune, and antivirus activity. Furthermore, IFITM3 gene has been considered as a preferential marker for inflammatory diseases, and positive correlation to pathological grades. Therefore, we assumed that IFITM3 was regulated by different signal pathways. To better understand IFITM3 function in inflammatory response, we cloned swine IFITM3 gene, and detected IFITM3 distribution in tissues, as well as characterized this gene. Results indicated that the length of swine IFITM3 gene was 438 bp, encoding 145 amino acids. IFITM3 gene expression abundance was higher in spleen and lungs. Moreover, we next constructed the eukaryotic expression vector PBIFM3 and transfected into PK15 cells, finally obtained swine IFITM3 gene stable expression cell line. Meanwhile, we explored the effects of LPS on swine IFITM3 expression. Results showed that LPS increased IFITM3 mRNA abundance and exhibited time-dependent effect for LPS treatment. To further demonstrate the mechanism that IFITM3 regulated type I IFNs production, we also detected the important molecules expression of TLR4 signaling pathway. In transfected and non-transfected IFITM3 PK15 cells, LPS exacerbated the relative expression of TLR4-NFκB signaling molecules. However, the IFITM3 overexpression suppressed the inflammatory development of PK15 cells. In conclusion, these data indicated that the overexpression of swine IFITM3 could decrease the inflammatory response through TLR4 signaling pathway, and participate in type I interferon production. These findings may lead to an improved understanding of the biological function of IFITM3 in inflammation.

IFITM proteins: Understanding their diverse roles in viral infection, cancer, and immunity

“…Tantalizingly, another study has provided evidence that IFITM3 may promote the autophagic degradation of IRF3, leading to reduced type I IFN production upon Sendai virus infection or poly(I:C) treatment ( 89 ). These data may also be consistent with the observation that swine IFITM3 overexpression appears to regulate IFNα/IFNβ mRNA levels upon lipopolysaccharide treatment in a swine kidney cell line ( 90 ). Taken together, these findings imply a potentially general anti-inflammatory role for intracellular IFITM3 (other IFITM proteins were not investigated in these studies).…”

Disabling of nephrogenesis in porcine embryos via CRISPR/Cas9‐mediated SIX1 and SIX4 gene targeting

“…Interestingly, the recent development of genome editing using clustered regulatory interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas9) technology and its combination with somatic cell nuclear transfer (SCNT) has allowed the creation of a number of useful pig models, thereby confirming that the CRISPR/Cas9‐mediated genome editing system can be used effectively in pigs . Similarly, the availability of porcine kidney PK15 epithelial cells, which have a wide range of applications in scientific research, including cell transfection, virus infection and vaccine production, makes them ideal candidates for investigating the signalling pathways of kidney development through in vitro experiments.…”

Identification of genes underlying the enhancement of immunity by a formula of lentinan, pachymaran and tremelia polysaccharides in immunosuppressive mice

“…Interferon inducible trans-membrane protein 3 (IFITM3, gene ID 12655) is a double trans-membrane protein belonging to the IFITM family and is primarily stimulated by interferons, participating in cell adhesion, immune, and antivirus activity and inflammatory diseases 37 . We therefore hypothesize that polysaccharides could increase these functions by regulating IFITM3.…”