Characterization and Antidermatophyte Activity of Henna Extracts: A Promising Therapy for Humans and Animals Dermatophytoses

Taha, Mohammed; Tartor, Yasmine H.; Abdul-Haq, Souheir I. M.; El-Maati, Mohamed F. Abo

doi:10.1007/s00284-021-02686-4

Cited by 4 publications

(4 citation statements)

References 44 publications

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“…Antimycotic activity as MICs and MFCs of extracts was further confirmed through SEM's altered architecture of the dermatophytes under study. In accordance with our results, Taha et al [5], by using scanning electron microscopy (SEM) analysis, revealed the morphological changes in hyphae of 30 clinical dermatophytes, including Trichophyton and Microsporum species exposed to ethanolic and acetone extracts of henna leaves and these structural changes including disintegrating, distortion, twisty, swollen, crushing of hyphae with waving and depressing on hyphal surfaces at 25 to 75 μg/mL of ethanol or acetone extract.…”

Section: Discussionsupporting

confidence: 92%

“…It should be preferred over other methods for its unparalleled image quality, resolution, magnification, actual sample structure preservation, and minimal sample loss [24]. As previously reported by Taha et al [5], morphological changes in hyphae of 30 clinical dermatophyte isolates, including Trichophyton and Microsporum species, following exposure to ethanolic and acetone extracts of henna leaves such as collapsing, distortion, twisting, inflating, bulging, and crushing of hyphae with corrugation of walls. Depressions on hyphal surfaces and distortion of hyphae at 25 to 75 μg/mL of ethanol or acetone extract were observed using scanning electron microscopy (SEM) analysis.…”

Section: Introductionmentioning

confidence: 93%

“…As a result, natural products with anticancer qualities have received a lot of attention since they are safe, low-cost, and widely accessible. In addition, they contain many biological and therapeutic activities that may promote health and relieve cancer [1,5].…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Screening, Antifungal, and Anticancer Activities of Medicinal Plants Thymelaea Hirsuta, Urginea Maritima, and Plantago Albicans

El-Bondkly

Shammari

El-Gendy

et al. 2022

BioMed Research International

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Ethyl acetate, ethanol, and acetone extracts of the medicinal plants Thymelaea hirsuta L., Urginea maritima L., and Plantago albicans L. (aerial parts) were evaluated for their phytochemical compositions, antimycotic activity against dermatophytes, and antiproliferative activity against different human cancer cell lines. Among them, the ethanolic extracts showed the highest phytochemical contents along with hyperactivities and were then selected for gas chromatography-mass spectrometry and Fourier-transform infrared spectroscopy analysis. The Fourier-transform infrared spectroscopy analysis confirmed the presence of different characteristic peak values with various functional chemical groups of the active components. However, U. maritima extracts through Fourier-transform infrared spectroscopy analysis showed distinctive peaks related to phenolic, amines, amides, aromatic, alkanes, alkyne, cyclopentanone, conjugated aldehyde, nitro, methoxy, uronic acids, aromatic esters, tertiary alcohol or ester, secondary and primary alcohols, aliphatic ether, sulfoxide, vinylidene, and halo compounds. Many bioactive main compounds with reported biological activities were detected by GC/MS (%) in the ethanolic extract of T. hirsuta, U. maritima, and P. albicans. All studied dermatophytes included a diverse set of virulence factors, including phospholipase, protease, keratinase, hemolysis, and melanoid production, all of which play vital roles in dermatophytic infection. Ethanolic extract of P. albicans inhibited the growth of Trichophyton soudanense totally and Trichophyton erinacei in addition to all Microsporum species. In contrast, the ethanolic extract of Trichophyton hirsuta at concentrations of 25 g/mL totally prevented the growth of all Trichophyton species. EtOH extract of U. maritima completely prevented the growth (100% inhibition) of all dermatophytic strains under study at the lowest concentration of 12.5 μg/mL. Scanning electron microscope analysis revealed considerable morphological modifications and structural alterations in dermatophyte species exposed to ethanolic extract of these plants. The viability of HCT-116, HepG-2, MCF-7, and HeLa cell lines was reduced after treatment with the ethanolic extracts of T. hirsuta, U. maritima, and P. albicans individually with IC50 values (10.0, 9.97, 48.5, and 56.24 μg/mL), (26.98, 25.0, 17.11, and 9.52 μg/mL), and (9.32, 7.46, 12.50, and 16.32 μg/mL), respectively. Our work revealed the significance of these traditional ethnomedical plants as potent sources for biologically active pharmaceuticals with potential applicability for the treatment of fungal and cancer diseases.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

See 1 more Smart Citation

Phytochemical Screening, Antifungal, and Anticancer Activities of Medicinal Plants Thymelaea Hirsuta, Urginea Maritima, and Plantago Albicans

El-Bondkly

Shammari

El-Gendy

et al. 2022

BioMed Research International

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“…The test was performed in triplicate and a negative control (tween 20) and a positive control (10 µg itraconazole disc; Hi-Media Laboratories, Mumbai, India) were included. The diameter of the zones of inhibition was measured and recorded after 96 h of incubation at 25 °C, and the mean values were interpreted as sensitive (inhibition zone ≥ 10 mm) [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Development, Optimization, and In Vitro/In Vivo Evaluation of Azelaic Acid Transethosomal Gel for Antidermatophyte Activity

et al. 2023

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Treatment of dermatophytosis is quite challenging. This work aims to investigate the antidermatophyte action of Azelaic acid (AzA) and evaluate its efficacy upon entrapment into transethosomes (TEs) and incorporation into a gel to enhance its application. Optimization of formulation variables of TEs was carried out after preparation using the thin film hydration technique. The antidermatophyte activity of AzA-TEs was first evaluated in vitro. In addition, two guinea pig infection models with Trichophyton (T.) mentagrophytes and Microsporum (M.) canis were established for the in vivo assessment. The optimized formula showed a mean particle size of 219.8 ± 4.7 nm and a zeta potential of −36.5 ± 0.73 mV, while the entrapment efficiency value was 81.9 ± 1.4%. Moreover, the ex vivo permeation study showed enhanced skin penetration for the AzA-TEs (3056 µg/cm2) compared to the free AzA (590 µg/cm2) after 48 h. AzA-TEs induced a greater inhibition in vitro on the tested dermatophyte species than free AzA (MIC90 was 0.01% vs. 0.32% for T. rubrum and 0.032% for T. mentagrophytes and M. canis vs. 0.56%). The mycological cure rate was improved in all treated groups, specially for our optimized AzA-TEs formula in the T. mentagrophytes model, in which it reached 83% in this treated group, while it was 66.76% in the itraconazole and free AzA treated groups. Significant (p < 0.05) lower scores of erythema, scales, and alopecia were observed in the treated groups in comparison with the untreated control and plain groups. In essence, the TEs could be a promising carrier for AzA delivery into deeper skin layers with enhanced antidermatophyte activity.

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Isolation of coumarins with anti-Trichophyton rubrum activity from Heracleum vicinum Boiss.

Liu

et al. 2023

Braz J Microbiol

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Characterization and Antidermatophyte Activity of Henna Extracts: A Promising Therapy for Humans and Animals Dermatophytoses

Cited by 4 publications

References 44 publications

Phytochemical Screening, Antifungal, and Anticancer Activities of Medicinal Plants Thymelaea Hirsuta, Urginea Maritima, and Plantago Albicans

Phytochemical Screening, Antifungal, and Anticancer Activities of Medicinal Plants Thymelaea Hirsuta, Urginea Maritima, and Plantago Albicans

Development, Optimization, and In Vitro/In Vivo Evaluation of Azelaic Acid Transethosomal Gel for Antidermatophyte Activity

Isolation of coumarins with anti-Trichophyton rubrum activity from Heracleum vicinum Boiss.

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