Characterization and antimicrobial susceptibility of coagulase-negative staphylococci isolated from clinical samples

Abstract

PURPOSE: This study has been done to speciate coagulase-negative staphylococci (CoNS) and also study their antibiotic susceptibility pattern isolated from clinical samples. MATERIALS AND METHODS: A total of 120 consecutive CoNS were isolated from various clinical samples such as blood, pus, wound swab, drain fluid, tracheal aspirate, peritoneal fluid, and pleural fluid over a period of 6 months. CoNS were identified by characteristic growth on media such as Blood agar and MacConkey agar. Speciation and identification were done by a range of biochemical testing such as PYR broth hydrolysis, novobiocin resistance, polymyxin B sensitivity, and then by matrix-assisted laser desorption ionization-time of flight. Antibiotic susceptibility of the isolates was done by Kirby-Bauer disk diffusion method as per CLSI 2017 guidelines. RESULTS: Among the 120 isolates, the most common species was Staphylococcus epidermidis (56.67%) followed by Staphylococcus haemolyticus (21.67%), Staphylococcus lugdunensis (11.67%), Staphylococcus caprae (5%), Staphylococcus cohnii (3.33%), and finally Staphylococcus vitulinus (1.67%). Good in vitro susceptibility was noted toward linezolid (100%), vancomycin (100%), teicoplanin (100%), and doxycycline (80.2%). The antibiotics to which resistance was seen were penicillin (96.5%), ciprofloxacin (57.1%), and oxacillin (45.5%). MR CoNS in our study ranged from 50% to 68.67%. CONCLUSION: Antibiotic resistance in CoNS is increasing toward penicillin, ciprofloxacin, and oxacillin as found in our study. The antibiotics such as vancomycin, teicoplanin, linezolid, and doxycycline which showed good in vitro susceptibility, therefore, should be kept as reserve drugs and used judiciously.

Prevalence and Antibiotic Susceptibility of Coagulase Negative Staphylococci in Tertiary Care Centre

“…This is followed by S.saprophyticus & S.haemolyticus and S.hominis, S.lugdenensis and finally S.xylosus. Our results are consistent with studies performed by Prapti Bora et al, [3], Akinkunmi E OA et al, [11], de Oliveira et al, [12], Sheikh A MM et al, [13]. CONS have been isolated from various medical devices used in these units, from NICU environment and paediatric wards [9].CONS are more prevalent in female population with 61% than in males with 39%.The prevalence of CONS is more common among people of age groups between 20-39yrs followed by people of age group between 40-59yrs, followed by 60-79yrs ,followed by 0-19 yrs.…”

Antimicrobial resistance profiles of bacterial pathogens isolated from the bloodstream in patients of a hospital institution in Montería — Córdoba

“…Pseudomonas aeruginosa showed high sensitivity to most of the drugs used, however it showed high resistance to doripenem (50), imipenem (56.2) and meropenem (43.8). Comparing the results obtained with another study conducted in Latin America (Argentina, Brazil, Chile, Costa Rica, Ecuador, Guatemala, Mexico, Panama, Peru and Venezuela, Colombia), it was possible to observe a similarity in the results with a resistance to imipenem (44.9%), meropenem (38.4%) and doripenen (49%) (35)(36)(37) . This may be due to empirical and inappropriate use as a first-line treatment.…”

Pathogenic Bacteria Contamination along the Beef line at Asella Municipal Abattoir, Arsi Zone Oromia Regional State, Ethiopia

“…Coagulase-negative staphylococci comprise a large group of related species commonly found on the surface of healthy persons, in whom they are opportunistic pathogens that cause infection in debilitated or compromised patients (Kayser, 2005). The pathogenic potential in food poisoning, as well as reservoirs for drug resistant genes (Bora et al, 2018) The present high rate of E. coli (10.9%) in the studied abattoir line, in which all sampling locations were found contaminated with E. coli at rate ranging from 10.0-30.0% indicated the possible presence of faulty evisceration, contaminated working surfaces and/or handling of the meat contaminated handlers. E. coli were frequently isolated from meat and the handling environment in Ethiopia (Tassew et al, 2010;Dagnew et al, 2013;Beyene et al, 2017).…”