Characterization and Antioxidant Activity of Essential Oil of Four Sympatric Orchid Species

Cuna, Francesco Saverio Robustelli della; Calevo, Jacopo; Bari, Elia; Giovannini, A.; Boselli, Cinzia; Tava, Aldo

doi:10.3390/molecules24213878

Cited by 27 publications

(24 citation statements)

References 44 publications

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“…The ROS-scavenging activity of SNPs was evaluated by the DPPH colorimetric assay as previously reported [13,18,32,33] with slight modifications. Briefly, each SNP formulation was tested considering different final concentrations in the final reaction mix (15, 10, 5 and 2.5 mg/mL) and their free drug equivalent concentration calculated from the loading data.…”

Section: Ros-scavenging Activitymentioning

confidence: 99%

Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

et al. 2020

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Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs.

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Section: Ros-scavenging Activitymentioning

confidence: 99%

Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

et al. 2020

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“…While research is still needed to identify pollinators, our analyses constitute a first contribution for the study of compounds possibly involved in plant-animal interactions. However, other functions of floral volatiles, that may play a crucial role in herbivory avoidance and as defensive molecules against pathogens, cannot be excluded [ 35 , 36 ]. Differences in the floral scents of related taxa could play a role in reproductive isolation by influencing pollinator’s behavior and choices [ 37 , 38 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Flowers ( D. amabile 25 g, D. chrysanthum 5.53 g, D. chrysotoxum 5.87 g, D. harveyanum 6.14 g, D. wardianum 6.26 g), to which octyl octanoate (98%, Sigma-Aldrich, Inc., St. Louis, MO, USA) was added as internal standard, were steam distilled with odor-free water for 3 h. The distillate was extracted with methylene chloride (3 × 100 mL) (Merck, Darmstadt, Germany), dried over anhydrous sodium sulfate (Sigma-Aldrich, Inc., St. Louis, MO, USA), and concentrated at first with a rotary evaporator and subsequently using a gentle stream of N 2 for successive GC/FID and GC/MS analyses [ 23 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition of Essential Oil from Flowers of Five Fragrant Dendrobium (Orchidaceae)

Cuna

Calevo

Bazzicalupo

et al. 2021

Plants

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A detailed chemical composition of Dendrobium essential oil has been only reported for a few main species. This article is the first to evaluate the essential oil composition, obtained by steam distillation, of five Indian Dendrobium species: Dendrobium chrysotoxum Lindl., Dendrobium harveyanum Rchb.f., and Dendrobium wardianum R.Warner (section Dendrobium), Dendrobium amabile (Lour.) O’Brien, and Dendrobium chrysanthum Wall. ex Lindl. (section Densiflora). We investigate fresh flower essential oil obtained by steam distillation, by GC/FID and GC/MS. Several compounds are identified, with a peculiar distribution in the species: Saturated hydrocarbons (range 2.19–80.20%), organic acids (range 0.45–46.80%), esters (range 1.03–49.33%), and alcohols (range 0.12–22.81%). Organic acids are detected in higher concentrations in D. chrysantum, D. wardianum, and D. harveyanum (46.80%, 26.89%, and 7.84%, respectively). This class is represented by palmitic acid (13.52%, 5.76, and 7.52%) linoleic acid (D. wardianum 17.54%), and (Z)-11-hexadecenoic acid (D. chrysantum 29.22%). Esters are detected especially in species from section Dendrobium, with ethyl linolenate, methyl linoleate, ethyl oleate, and ethyl palmitate as the most abundant compounds. Alcohols are present in higher concentrations in D. chrysantum (2.4-di-tert-butylphenol, 22.81%), D. chrysotoxum (1-octanol, and 2-phenylethanol, 2.80% and 2.36%), and D. wardianum (2-phenylethanol, 4.65%). Coumarin (95.59%) is the dominant compound in D. amabile (section Densiflora) and detected in lower concentrations (range 0.19–0.54%) in other samples. These volatile compounds may represent a particular feature of these plant species, playing a critical role in interacting with pollinators.

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“…The ROS-scavenging activity of sericin-based nanoparticles was evaluated by the DPPH (2,2-diphenyl-2-picrylhydrazyl hydrate) assay, according to [38,39]. Briefly, NPg and NPc were tested at three concentrations: 2.5, 5, and 10 mg/mL (aqueous suspensions).…”

Section: Ros-scavenging Activity Assaymentioning

confidence: 99%

Crocetin as New Cross-Linker for Bioactive Sericin Nanoparticles

et al. 2021

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The nose-to-brain delivery route is used to bypass the blood–brain barrier and deliver drugs directly into the brain. Over the years, significant signs of progress have been made in developing nano-drug delivery systems to address the very low drug transfer levels seen with conventional formulations (e.g., nasal solutions). In this paper, sericin nanoparticles were prepared using crocetin as a new bioactive natural cross-linker (NPc) and compared to sericin nanoparticles prepared with glutaraldehyde (NPg). The mean diameter of NPc and NPg was about 248 and 225 nm, respectively, and suitable for nose-to-brain delivery. The morphological investigation revealed that NPc are spherical-like particles with a smooth surface, whereas NPg seem small and rough. NPc remained stable at 4 °C for 28 days, and when freeze-dried with 0.1% w/v of trehalose, the aggregation was prevented. The use of crocetin as a natural cross-linker significantly improved the in vitro ROS-scavenging ability of NPc with respect to NPg. Both formulations were cytocompatible at all the concentrations tested on human fibroblasts and Caco-2 cells and protected them against oxidative stress damage. In detail, for NPc, the concentration of 400 µg/mL resulted in the most promising to maintain the cell metabolic activity of fibroblasts higher than 90%. Overall, the results reported in this paper support the employment of NPc as a nose-to-brain drug delivery system, as the brain targeting of antioxidants is a potential tool for the therapy of neurological diseases.

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Characterization and Antioxidant Activity of Essential Oil of Four Sympatric Orchid Species

Cited by 27 publications

References 44 publications

Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

Chemical Composition of Essential Oil from Flowers of Five Fragrant Dendrobium (Orchidaceae)

Crocetin as New Cross-Linker for Bioactive Sericin Nanoparticles

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