Characterization and application of chemical-resistant polyurethane-based enzyme and whole cell compartments

Uhrich, Diana; Jang, Hyun Young; Park, Jin Byung; Langermann, Jan von

doi:10.1016/j.jbiotec.2018.11.007

Cited by 4 publications

(5 citation statements)

References 46 publications

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“…Additionally, such static emulsions containing separated reaction zones were produced for enzymatic cascade reactions catalyzed by different enzymes requiring contrary reaction conditions. Moreover, the same research group also developed polyurethane‐based static emulsions that entrap entire cells overexpressing alcohol dehydrogenase or esterase for biocatalytic synthesis [97] . This catalysis system shows considerable stability, allowing multiple reuses without a noticeable decline in catalytic activity.…”

Section: Polymeric Reactors In Biphasic Solutionsmentioning

confidence: 99%

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

et al. 2022

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Biocatalysis is increasingly being explored for the sustainable development of green industry. Though enzymes show great industrial potential with their high efficiency, specificity, and selectivity, they suffer from poor usability and stability under abiological conditions. To solve these problems, researchers have fabricated nano-and micro-sized biocatalytic reactors based on the self-assembly of various polymers, leading to highly stable, functional, and reusable biocatalytic systems. This Review highlights recent progress in self-assembled polymeric nano-and microreactors for biocatalytic synthesis, including polymersomes, reverse micelles, polymer emulsions, Pickering emulsions, and static emulsions. We categorize these reactors into monophasic and biphasic systems and discuss their structural characteristics and latest successes with representative examples. We also consider the challenges and potential solutions associated with the future development of this field.

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Section: Polymeric Reactors In Biphasic Solutionsmentioning

confidence: 99%

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

et al. 2022

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“…Moreover, the same research group also developed polyurethane-based static emulsions that entrap entire cells overexpressing alcohol dehydrogenase or esterase for biocatalytic synthesis. [97] This catalysis system shows considerable stability, allowing multiple reuses without a noticeable decline in catalytic activity.…”

Section: Static Emulsionsmentioning

confidence: 99%

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

et al. 2022

View full text Add to dashboard Cite

Biocatalysis is increasingly being explored for the sustainable development of green industry. Though enzymes show great industrial potential with their high efficiency, specificity, and selectivity, they suffer from poor usability and stability under abiological conditions. To solve these problems, researchers have fabricated nano‐ and micro‐sized biocatalytic reactors based on the self‐assembly of various polymers, leading to highly stable, functional, and reusable biocatalytic systems. This Review highlights recent progress in self‐assembled polymeric nano‐ and microreactors for biocatalytic synthesis, including polymersomes, reverse micelles, polymer emulsions, Pickering emulsions, and static emulsions. We categorize these reactors into monophasic and biphasic systems and discuss their structural characteristics and latest successes with representative examples. We also consider the challenges and potential solutions associated with the future development of this field.

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“…Many methods are known, for example immobilization of whole cells by entrapment in polymers, hydrogels, or other materials. To give an example for the entrapment of biocatalysts in polymers, von Langermann et al developed a technique of whole-cell immobilization in polyurethane, which is a suitable heterogenized biocatalyst with a higher stability when compared to free whole cells [16][17][18]. Whole-cell catalysts (or free enzymes [19,20]) in an aqueous medium can also be immobilized by entrapment in superabsorber, yielding in a solid aqueous phase which can be used in an organic solvent as reaction medium and easily separated by filtration [21].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Aldoxime Dehydratases and Their Use as Biocatalysts in Aqueous Reaction Media

2020

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Immobilization of biocatalysts is a current topic in research enabling the easy recovery of catalysts from the reaction medium after the reaction, and it is often accompanied by a stabilization of the catalysts, which enables recycling. Within our ongoing research on the utilization of aldoxime dehydratases in the cyanide-free synthesis of nitriles through dehydration of readily available aldoximes, a screening of different immobilization methods for free enzymes was performed. The applied immobilization methods are based on covalent binding and hydrophobic interactions of the enzyme with the carrier material and whole-cell immobilization in calcium alginate beads with and without subsequent coating. In our study, we found that the immobilization with purified free aldoxime dehydratases from OxdRE (Rhodococcus erythropolis) and OxdB (Bacillus sp. strain OxB-1) leads to high immobilization efficiencies, but also to a strong loss of activity with a residual activity of <20%, regardless of the carrier material used. However, when using whole cells for immobilization instead of purified enzymes, we could increase the residual activity significantly. Escherichia coli BL21(DE3)-CodonPlus-RIL OxdRE and OxdB whole cells were entrapped in calcium alginate beads and coated with silica using tetraethylorthosilicate (TEOS), leading to immobilized catalysts with up to 75% residual activity and a higher stability compared to the free whole cells. Even after three rounds of recycling, which corresponds to a 3 d reaction time, the immobilized OxdB whole cells showed a residual activity of 85%.

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Characterization and application of chemical-resistant polyurethane-based enzyme and whole cell compartments

Cited by 4 publications

References 46 publications

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

Immobilization of Aldoxime Dehydratases and Their Use as Biocatalysts in Aqueous Reaction Media

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