Characterization and application of immobilized lipase enzyme on different radiation grafted polymeric films: Assessment of the immobilization process using spectroscopic analysis

Abstract

Lipase has been immobilized onto different films, polypropylene and poly(tetrafluoroethylene-perfluroro-propyl vinyl ether) using glutalaradehyde as a crosslinker. Differential scanning calorimetery, Fourier transform infrared spectroscopic, x-ray diffraction, and scanning electron microscopy measurements were carried out to confirm the structure of the polymer films as well as the immobilization process of the enzyme onto the polymeric carrier. The activity and stability of the resulting biopolymers produced by lipase have been compared to those for the native lipase. The experimental results showed that the optimum temperature and pH were 40°C and 8.0, respectively. The activity of the immobilized lipases varied with lipase concentration and with the yield of grafting. Subjecting the immobilized enzymes to a dose of ␥-radiation of (0.5-10 Mrad) showed complete loss in the activity of the free enzyme at a dose of 5 Mrad. A leakage of the enzyme from the irradiated membranes was not observed in the repeated batch enzyme reactions. The operational stability of the free and immobilized lipase in n-hexane showed that the immobilized enzyme was much more stable than the free one.

Preparation of glucose oxidase immobilized in different carriers using radiation polymerization

“…It was observed that the structure of modified PVA allows the local pH value to be maintained at a level different from the pH of the external medium. 23,24 The immobilized glucose oxidase preparations exhibited high stability against inactivation caused by various water-miscible organic solvents, dioxan, acetone and tetrahydrofuran. Figure 4 shows that the enzyme immobilized using the covalent binding technique [GOD-immobilized onto epichlorohydrin-agarose (Prep II)] exhibited more resistance to solvent-induced inactivation compared with the other preparation prepared by the radiation-entrapment technique [GOD-immobilized in PVA/PAAm hydrogel (Prep I)].…”

Characterization and application of grafted polypropylene and polystyrene treated with epichlorohydrin coupled with cellulose or starch for immobilization processes

“…Also, the pH optimum of α‐amylase did not change on immobilization. This implied that the immobilization of α‐amylase did not affect the enzyme‐active site 2…”

“…Radiation‐induced graft copolymerization is a well‐known method used to introduce functional groups into different polymer materials with specially selected monomers. There have been several reports on the use of radiation graft copolymerization of polar monomers onto polymer films to obtain hydrophilic properties for versatile applications 1–5. Grafting copolymerization performed by the radiation method is one useful method because of its uniform and rapid creation of active radical sites 6, 7.…”

Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis

“…Nanoporous poly( N ‐ isopropylacrylamide) (PNIPAAm) membranes have been fabricated in glass microchannels to substantially enrich and purify nucleic acids through photopatterning technology . Lipase has been immobilized onto different polymeric films, such as polypropylene and the process have been assessed by spectroscopic analysis . Polymeric films in a microchip are useful for high‐density enzyme immobilization .…”

“…9 Lipase has been immobilized onto different polymeric films, such as polypropylene and the process have been assessed by spectroscopic analysis. 10 Polymeric films in a microchip are useful for high-density enzyme immobilization. 11 Polymer-based materials, including polydimethylsiloxane, poly(methyl methacrylate), polycarbonate and Teflon, have been extensively used to fabricate microchips using photolithography, soft lithography and micromachining using lasers or microdrilling.…”