Characterization and Assay of Steroid Hormone Receptors and Steroid-Binding Serum Proteins by Agargel Electrophoresis at Low Temperature

mentioning

confidence: 99%

“…In conclusion, the histochemical use of lectins (Wagner, 1972) allows a distinction to be made between hormone sensitive and non-sensitive tumours and has the advantage over hormone-receptor analysis in that it can be carried out on fixed tissue and on very small amounts of tumour material (e.g. metastases).…”

mentioning

confidence: 99%

Secretion-associated lectin-binding sites as a parameter of hormone dependence in mammary carcinoma

Klein¹,

Vierbuchen²,

Würz³

et al. 1981

“…The incubations were continued overnight at 00C. They were then subjected to charcoal/ dextran separation as described above and the supernatants were applied to agar gel for electrophoresis as described by Wagner (1972). The gels were cut, and each fraction placed in a counting vial containing 10 ml of toluene containing 0.3% 2,5-diphenyl oxazole (PPO) and 0.01% 1,4-bis-2-(-4-methyl-5-phenyl oxazole)-benzene (POPOP).…”

mentioning

confidence: 99%

“…The difference between the radioactivity recovered from the "anodal receptor fraction" (Wagner, 1972) Progesterone-receptor assay.-Two 500il aliquots were taken from the same cytosol preparation as used for the oestradiolreceptor assay. The aliquots were incubated for 20 min at 0°C in the presence of excess unlabelled cortisol (final concentration 6 x 10-6M).…”

mentioning

confidence: 99%

25-Hydroxycholecalciferol receptors in human breast cancer

Murphy¹,

Wild²,

Posen³

et al. 1979

“…The LS A-protein activity in the same tumour system was found to be high in both dependent and independent tumours, while LS B-protein was non- (1966) showed that this contamination varied from 10 to 20%. The first 20 tumour specimens were also tested for oestradiol binding capacity using the agar gel electrophoresis method (Wagner, 1972 Glock and McLean (1953) as described by Briand and Daehnfeldt (1973) by monitoring the formation of NADPH at 340 nm in an assay volume of 1-0 ml. Results were expressed as ng/mg supernatant protein.…”

mentioning

confidence: 99%

High affinity oestradiol receptors and the activity of glucose-6-phosphate dehydrogenase and lactose synthetase in mammary carcinomata of postmenopausal women

Daehnfeldt¹,

Schülein²

1975

Summary.-The determination of hormone inducible proteins in endocrine tumours may yield information about the presence of hormone dependent tumour cells. We have estimated the high affinity oestradiol binding capacity in primary mammary carcinomata of 57 postmenopausal patients. Glucose-6-phosphate dehydrogenase and lactose synthetase are known from animal experiments to be hormone inducible. Therefore, in biopsies of sufficient size the activity of glucose-6-phosphate dehydrogenase (47 patients) and lactose synthetase (23 patients) was also studied. It was found that biopsies with high binding capacity also showed high activities of glucose -6-phosphate dehydrogenase and lactose synthetase A protein (galactosyl transferase). No lactose synthetase B protein (a-lactalbumin) has been discovered in the tumours. The present observations may be considered suggestive evidence of a relationship between high oestradiol binding capacity and high activities of the two enzymes on the one hand and hormone dependence of the tumour on the other. However, further clinical studies are required before final conclusions in this respect can be drawn.