Characterization and Association Analysis with Litter Size Traits of Porcine Matrix Metalloproteinase-9 Gene (pMMP-9)

Abstract

The mammalian matrix metalloproteinase-9 (MMP-9), which might play a role in ovulation, uterus remodeling, embryo development, and implantation in mammals, is one of the potential functional candidate genes for porcine reproductive traits. In this study, the entire genomic sequence of porcine MMP-9 (pMMP-9) gene was established; it contains 13 exons and 12 introns. Real-time PCR analysis revealed that pMMP-9 is highly expressed in the Minzhu uterus before puberty and decreases significantly after sexual maturity (p < 0.05). Two single-nucleotide polymorphisms (A3011G and T5079C) that can be detected by PCR restriction fragment length polymorphism (PCR-RFLP) were discovered and tested for statistical associations with litter size traits in a crossbred population (Line DIV) derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan pigs. For A3011G, the GG genotype was associated with a significantly higher (p < 0.05) number of live births than those recorded for AA sows and the additive effect was significant (p < 0.05). The T5079C marker is not associated with litter size in this population. Further studies are needed to confirm the results of this study.

How matrix metalloproteinase (MMP)-9 (rs3918242) polymorphism affects MMP-9 serum concentration and associates with autism spectrum disorders: A case-control study in Iranian population

“…MMP-9 is an enzyme that plays a critical role in synaptic plasticity and brain development (Stawarski, Stefaniuk, & Wlodarczyk, 2014). MMP-9 contains 12 introns and 13 exons (Niu, Li, Xiong, & Wang, 2013) and in human MMP-9 comprises 7,654 bases and is located on chromosome 20 (20q11.2-q13.1) (Farina & Mackay, 2014). According to its structure, MMP-9 has many substrates including cell surface receptors, gelatin, growth factors precursors, adhesion molecule, procollagen type II, tissue inhibitors of metalloproteinases (TIMPs) specially TIMP-1, and laminin, which is capable of connecting with them (Bronisz & Kurkowska-Jastrzębska, 2016).…”

Polymorphism, Genetic Effect and Association with Egg Production Traits of Chicken Matrix Metalloproteinases 9 Promoter

“…Our results showed that C − allele has positive effects on egg production and the (−1,954 C + /C − ) polymorphism could be used as a selection marker in chicken breeding to improve laying performance. Comparatively, MMP9 gene polymorphisms were also associated with the litter size traits in pig populations ( Niu et al, 2013 ). Many of the biological aspects of MMP9, including catalytic and non-catalytic activities, as well as specific MMP9 substrates have been implicated in reproduction biology ( Vandooren et al, 2013 ).…”

Integrated analysis of proteomics and metabolomics in girls with central precocious puberty

“…In addition, MMP9, TIMP1, SPP1 and POSTN were shown to play a role in regulating fat metabolism and insulin resistance ( 30 , 36 – 38 ). MMP9, TIMP1, CDC42 and BMP1 were also involved in follicular development ( 39 – 43 ), suggesting that these proteins may be closely related to pubertal development. However, these proteins had not been previously reported to be related to CPP, and their mechanisms needs to be further studied.…”