Polymorphism, Genetic Effect and Association with Egg Production Traits of Chicken Matrix Metalloproteinases 9 Promoter

Abstract

Matrix metalloproteinases (MMP) are key enzymes involved in cell and tissue remodeling during ovarian follicle development and ovulation. The control of MMP9 transcription in ovarian follicles occurs through a core promoter region (−2,400 to −1,700 bp). The aim of this study was to screen genetic variations in the core promoter region and examine MMP9 transcription regulation and reproduction performance. A single cytosine deletion/insertion polymorphism was found at −1954 C+/C−. Genetic association analysis indicated significant correlation between the deletion genotype (C−) with total egg numbers at 28 weeks (p = 0.031). Furthermore, luciferase-reporter assay showed the deletion genotype (C−) had significantly lower promoter activity than the insertion genotype (C+) in primary granulosa cells (p<0.01). Therefore, the identified polymorphism could be used for marker-assisted selection to improve chicken laying performance.

Dynamic Changes in the Follicular Transcriptome and Promoter DNA Methylation Pattern of Steroidogenic Genes in Chicken Follicles throughout the Ovulation Cycle

“…The yolk in the SWF and F1 follicles was carefully removed with a syringe and a 25-gauge needle before snap frozen. The granulosa cells were isolated as described previously [ 8 , 9 ]. In brief, the second largest (F2), third largest (F3), and fourth largest (F4) follicles were isolated and punctuated with sterile needles to remove the yolk.…”

The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

“…A large number of Bacillus-based preparations have been found to promote growth, feed utilization and digestive health, subsequently, registered as probiotics for animal feed [51][52][53]. Egg production performance determines the economic benefits in laying hen production system owing to its effects on productivity [54]. Similar to our study, geese fed with 10 9 cfu g −1 Bacillus subtilis can increase growth performance and leg muscle weight (p < 0.05) because of its modulating the intestinal microflora ecology of the animal [55].…”

Transcriptome analysis of ovarian tissues highlights genes controlling energy homeostasis and oxidative stress as potential drivers of heterosis for egg number and clutch size in crossbred laying hens

“…Low expression of genes enriched in the ECM-receptor interaction pathway in this study suggests slow and perhaps more organized growth of the prehierarchical follicles in the crossbred hens ( Zhou et al, 2020 ). Specifically, 2 MMPs ( MMP9 and MMP10 ) with underdominance in the crossbred laying hens were linked to the degradation and resynthesis of extracellular matrix, the ovarian cycle, follicle development, and ovulation ( Oktay et al, 2000 ; Zhu and Jiang, 2014 ; Liu et al, 2018 ). High expression of these genes also promotes the rapture of ovarian follicles ( Liu et al, 2018 ) and a genome wide association study identified SNPs in the promoter region of MMP9 in chickens characterized by high egg production ( Zhu and Jiang, 2014 ).…”