Characterization and Bioactivities of a Novel Exopolysaccharide Produced from Lactose by Bacillus tequilensis PS21 Isolated from Thai Milk Kefir

Luang-In, Vijitra; Saengha, Worachot; Deeseenthum, Sirirat

doi:10.4014/mbl.1712.12018

Cited by 20 publications

(15 citation statements)

References 37 publications

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“…Similar reports were found in EPS produced by Rhodotorula glutinins which was composed of L-fucose and D-galactose (Singh et al 2012). Similar results were found in Bacillus tequilensis PS21 (Wu et al 2007;Luang-In et al 2018). XRD analysis showed partial crystalline nature of probiotic EPS.…”

Section: Discussionsupporting

confidence: 81%

Optimization of exopolysaccharide production by probiotic yeast Lipomyces starkeyi VIT-MN03 using response surface methodology and its applications

Ragavan

Das

2019

Ann Microbiol

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In the present study, the cultural conditions for exopolysaccharide (EPS) production from probiotic yeast Lipomyces starkeyi VIT-MN03 were optimized using response surface methodology (RSM) to maximize the yield of EPS. Interactions among the various factors viz. sucrose concentration (1-3 g%), NaCl concentration (2-4 g%), pH (3-5), temperature (20-30°C), and incubation period (20-40 days) during EPS production were studied using Box-Behnken design (BBD). The EPS was purified and characterized using various instrumental analyses. The properties like adhesion, antioxidant, biosurfactant, cholesterol removal, and binding ability to mutagens were also tested for EPS produced. Sixfold increase in EPS production (4.87 g L −1) by L. starkeyi VIT-MN03 was noted under optimized condition. EPS showed a high viscosity (1.8 Pa S −1) and good shearthinning properties. Instrumental analysis showed that EPS was heteropolysaccharide composed of glucan, mannan, and rhamnan. Lipomyces starkeyi VIT-MN03 exhibited good self-adhesion (95%) and co-aggregation ability (93%). Adhesion efficiency for yeast inoculum containing 5.5 × 10 7 CFU mL −1 per 9.2 cm 2 of Caco-2 cell (colorectal adenocarcinoma) was noted. The probiotic EPS displayed strong antioxidant ability to scavenge hydroxyl radical and DPPH by 58% and 71% respectively. In addition, biosurfactant activity (86%) and cholesterol removal (90%) ability of probiotic EPS was also tested. EPS bound cells of L. starkeyi VIT-MN03 showed good binding ability to mutagens. These results support the effectiveness of using RSM for maximum EPS production. To the best of our knowledge, this is the first report on optimization of EPS production by probiotic yeast.

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Section: Discussionsupporting

confidence: 81%

Optimization of exopolysaccharide production by probiotic yeast Lipomyces starkeyi VIT-MN03 using response surface methodology and its applications

Ragavan

Das

2019

Ann Microbiol

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Section: Introductionmentioning

confidence: 99%

“…These grains are made of colonies of microorganisms that synthesize an exopolysaccharide matrix. Grains can be grown on a variety of substrates such as milk (Ismail et al, 2018; Luang‐In et al, 2018; Sharifi et al, 2017), fruit juice (Plessas et al, 2016; Randazzo et al, 2016), cane molasses (Fiorda et al, 2017; Korukluoglu et al, 2017), and sugar (Y. T. Chen et al, 2018; Koh et al, 2018; Silva et al, 2009). Several studies have highlighted that kefir has the potential to treat diseases such as cancer (Hatmal et al, 2018; Melo et al, 2018; Topuz et al, 2008), gastritis (Akal et al, 2018; Bekar et al, 2011; Yamane et al, 2018), tumors (Yamane et al, 2018), inflammations (Rodrigues et al, 2016), and infections (Iraporda et al, 2017; Burlando & Cornara, 2017; Spinler et al, 2016; Prado et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

Matos

Pinto

Ramos

et al. 2020

Microscopy Res & Technique

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The aim of this study was to report the first discussion of statistical parameters and possible applications related to the surface of kefir biofilms prepared with Maytenus rigida Mart. extract through micromorphology, using atomic force microscopy (AFM). Four different samples were produced. The reference contained demerara sugar with a concentration of 40 g/L and the others had added extract whose concentrations were 0.25, 0.50, and 0.75 g/L, respectively. AFM was used to image the surface in tapping mode, on square areas of 30 μm × 30 μm. Images were studied by the MountainsMap premium commercial software. The main parameter of the surface, roughness, grew with the increase of the extract concentration up to 0.50 g/L. In addition, asymmetry and kurtosis showed that the biofilm with the best peak distribution was the one with 0.25 g/L of plant extract. The other parameters followed the main results for the surface and were observed for the depth and volume parameters. The power spectrum density spectrum confirmed the reliability of the results for the sample with 0.25 g/L. Therefore, the sample with 0.25 g/L of plant extract produced the best balance in relation to the surface properties. These results showed that the biofilm morphology and microtexture were affected by the incorporation of the plant extract and can be useful to define possible applications.

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“…A parallel increase between the EPS concentrations and the biomass was observed during the first 6 h of fermentation, reaching a maximum EPS yield of 15.61 g/L, then declined between 6 h and 12 h, and remained constant afterwards (Figure 6b). The time to obtain the maximum EPS was much shorter than those of B. tequilensis PS21 in a previous study [35], and the EPS yield of CGMCC 17603 (15.61 g/L) was more than that of B. tequilensis PS21, B. tequilensis FR9 (6.83 g/L), and B. sonorensis MJM60315 (8.4 g/L) [35,44,45], indicating that the production mode of CGMCC 17603 can greatly shorten the production time and increase the production efficiency in commercial production. Therefore, we have realized high-density culture of B. tequilensis CGMCC 17603 and improvement of EPS yield through low-cost, short-time, and simple production processes, and provided new solutions for the development of strain preparations and EPS preparations.…”

Section: Sand-fixing Property Of B Tequilensis Cgmcc 17603mentioning

confidence: 52%

“…High biomass yield and low cost in the production process are the keys to realizing the popularization and application of B. tequilensis CGMCC 17603 in the field of sand fixation. Our study optimized the culture conditions and medium compositions of CGMCC 17603, as these two were the most important factors affecting the production of bacterial biomass and metabolites [35][36][37][38][39]. As shown in Figure 3, the biomass of CGMCC 17603 peaked at temperature 31 • C, pH 8.5, agitation speed 230 rpm, and inoculation quantity 3%.…”

Section: High-density Culture Of B Tequilensis Cgmcc 17603mentioning

confidence: 99%

A New Strain of Bacillus tequilensis CGMCC 17603 Isolated from Biological Soil Crusts: A Promising Sand-Fixation Agent for Desertification Control

Zhao

Wang

et al. 2019

Sustainability

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In arid and semi-arid desert ecosystems, physical, chemical, and vegetative measures were used to prevent wind erosion. However, studies on the utilization of microbial resources for sand fixation are still limited. To fill this gap, a new strain of Bacillus tequilensis CGMCC 17603 with high productivity of exopolysaccharide (EPS) was isolated from biological soil crusts, and its high-density culture technology and sand-fixing ability were studied. The one-factor-at-a-time approach (OFAT) and Box–Behnken design of CGMCC 17603 showed that the optimum culture conditions were pH 8.5, temperature 31 °C, agitation speed 230 rpm, and inoculation quantity 3%, and the optimum medium was 27.25 g/L glucose, 15.90 g/L yeast extract, and 5.61 g/L MgSO4•7H2O. High-density culture showed that the biomass and EPS yield of CGMCC 17603 increased from 9.62 × 107 to 2.33 × 109 CFU/mL, and from 8.01 to 15.61 g/L, respectively. The field experiments showed that CGMCC 17603 could effectively improve the ability of sand fixation and wind prevention. These results indicated that B. tequilensis, first isolated from cyanobacterial crusts, can be considered as an ideal soil-fixing agent to combat desertification in arid and semi-arid areas.

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Characterization and Bioactivities of a Novel Exopolysaccharide Produced from Lactose by Bacillus tequilensis PS21 Isolated from Thai Milk Kefir

Cited by 20 publications

References 37 publications

Optimization of exopolysaccharide production by probiotic yeast Lipomyces starkeyi VIT-MN03 using response surface methodology and its applications

Optimization of exopolysaccharide production by probiotic yeast Lipomyces starkeyi VIT-MN03 using response surface methodology and its applications

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

A New Strain of Bacillus tequilensis CGMCC 17603 Isolated from Biological Soil Crusts: A Promising Sand-Fixation Agent for Desertification Control

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