Characterization and Biological Activity of a Novel Exopolysaccharide Produced by Pediococcus pentosaceus SSC–12 from Silage

Abstract

In this study, 22 strains of exopolysaccharides-producing lactic acid bacteria were isolated from silage, and the strain SSC–12 with the highest exopolysaccharide (EPS) production was used as the test strain. The SSC–12 was identified as Pediococcus pentosaceus, based upon 16S rDNA gene sequencing and Neighbor Joining (NJ) phylogenetic analysis. The analysis of the kinetic results of EPS generation of SSC–12 showed that the EPS generation reached the maximum value at 20 h of culture. The characterization study showed the EPS produced by SSC–12 was a homogeneous heteropolysaccharide comprising glucose (42.6%), mannose (28.9%), galactose (16.2%), arabinose (9.4%), and rhamnose (2.9%). The EPS had good antioxidant activity, especially the activity of scavenging hydroxyl free radicals. At the same time, the EPS also had strong antibacterial ability and could completely inhibit the growth of Staphylococcus aureus. The EPS produced by the Pediococcus pentosaceus SSC–12 can be used as a biologically active product with potential application prospects in the feed, food, and pharmaceutical industries.

Characteristics and Biological Activity of Exopolysaccharide Produced by Lysobacter sp. MMG2 Isolated from the Roots of Tagetes patula

“…So we compared our results with EPSs generated by different genera. While the DPPH scavenging activity of lyEPS at a concentration of 4 mg/mL was 89.25%, at a concentration of 10 mg/mL of SSC-12 EPS produced from Pediococcus pentosaceus SSC-12, the DPPH scavenging ability of SSC-12 EPS was 77.4%, which was lower than that of lyEPS DPPH scavenging activity [ 86 ]. The DPPH scavenging activity of lyEPS 4 mg/mL (89.25%) was higher than that of EPS produced by Pseudomonas fluorescens CrN6 (concentration, 4 mg/mL; DPPH scavenging activity, 45.4%) isolated from rhizosphere [ 87 ].…”

Assessment of Antibacterial Activity of Exopolysaccharide Produced from Pseudomonas aeruginosa strain S16 on Environmental and Clinical Bacteria

“…In a research study, Li et al [42] reported that the exopolysaccharides produced from Bi dobacterium bi dum WBIN03 and Lactobacillus plantarum R315 exhibited antibiotic activities against Staphylococcus aureus CGMCC26003 and other selected bacteria worked on, at a concentration of 300 µg/ml. In addition, Fan et al [43] reported a novel exopolysaccharide produced by Pediococcus pentocaseus SSC-12, which increased in antibacterial activity from 2 mg/ml through 6 mg/ml till it totally inhibited the growth of the strain of Staphylococcus aureus worked on, at a concentration of 10 mg/ml.…”

Relationship of Gene-Structure–Antioxidant Ability of Exopolysaccharides Derived from Lactic Acid Bacteria: A Review

“…Functional groups are critical components of the molecular backbone and have a pronounced influence on the antioxidant ability. EPS possessing more carboxyl functional groups provide an acidic environment and expose more hemiacetal groups, resulting in better antioxidant ability . The sulfate group increases the charge density of the carbon atom in the heterocycle, while the electron withdrawing carboxyl group reduces the hydrogen atom.…”

“…EPS possessing more carboxyl functional groups provide an acidic environment and expose more hemiacetal groups, resulting in better antioxidant ability. 47 The sulfate group increases the charge density of the carbon atom in the heterocycle, while the electron withdrawing carboxyl group reduces the hydrogen atom. This activates the sugar residue, resulting in an increase in the free radical scavenging ability.…”