Characterization and cytotoxic activity of apoptosis-inducing pierisin-5 protein from white cabbage butterfly

Subbarayan, Sarathbabu; Marimuthu, Satheesh Kumar; Kumar, Nachimuthu Senthil; Zhang, Wenqing; Subramanian, Selvi

doi:10.1016/j.ijbiomac.2016.01.072

Cited by 23 publications

(8 citation statements)

References 43 publications

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“…The pierisin toxin transfers an ADP-ribosyl moiety to 2'-deoxyguanosine residues in DNA 30 , while the ricin domains mediate interactions with neutral glycosphingolipid receptors, globotriaosylceramide (Gb3), and globotetraosylceramide (Gb4) 31 . The toxin is thought to serve as a defense factor against parasitization by wasps 12 , but also induces apoptosis in cancer cell lines 10, 11, 32 .…”

Section: Resultsmentioning

confidence: 99%

Complete genome of Pieris rapae, a resilient alien, a cabbage pest, and a source of anti-cancer proteins

et al. 2016

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The Small Cabbage White ( Pieris rapae) is originally a Eurasian butterfly. Being accidentally introduced into North America, Australia, and New Zealand a century or more ago, it spread throughout the continents and rapidly established as one of the most abundant butterfly species. Although it is a serious pest of cabbage and other mustard family plants with its caterpillars reducing crops to stems, it is also a source of pierisin, a protein unique to the Whites that shows cytotoxicity to cancer cells. To better understand the unusual biology of this omnipresent agriculturally and medically important butterfly, we sequenced and annotated the complete genome from USA specimens. At 246 Mbp, it is among the smallest Lepidoptera genomes reported to date. While 1.5% positions in the genome are heterozygous, they are distributed highly non-randomly along the scaffolds, and nearly 20% of longer than 1000 base-pair segments are SNP-free (median length: 38000 bp). Computational simulations of population evolutionary history suggest that American populations started from a very small number of introduced individuals, possibly a single fertilized female, which is in agreement with historical literature. Comparison to other Lepidoptera genomes reveals several unique families of proteins that may contribute to the unusual resilience of Pieris. The nitrile-specifier proteins divert the plant defense chemicals to non-toxic products. The apoptosis-inducing pierisins could offer a defense mechanism against parasitic wasps. While only two pierisins from Pieris rapae were characterized before, the genome sequence revealed eight, offering additional candidates as anti-cancer drugs. The reference genome we obtained lays the foundation for future studies of the Cabbage White and other Pieridae species.

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Section: Resultsmentioning

confidence: 99%

Complete genome of Pieris rapae, a resilient alien, a cabbage pest, and a source of anti-cancer proteins

et al. 2016

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“…A 100 µ L of MTT (5 mg/mL) was added to the cells for 4–5 hours, followed by a 15-minute addition of DMSO solution to dissolve the bottom layer of crystals. This was followed by the optical density (OD) measurement of the formazan product and calculation of the IC 50 [ 33 ].…”

Section: Materials Methodsmentioning

confidence: 99%

[Retracted] CuO‐TiO2‐Chitosan‐Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL‐2 in the Human K562 Cancer Cell Line

Elderdery

Alzahrani

Hamza

et al. 2022

Bioinorganic Chemistry and Applications

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In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO-TiO 2 -Chitosan-Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO/EtBr for apoptotic morphology assessment. The rate of apoptosis and cell cycle arrests was determined using flow cytometry. Flow cytometry was also employed to identify pro- and antiapoptotic proteins such as Bcl2, Bad, Bax, P53, and Cyt C. The FTIR spectrum revealed that the CuO-TiO 2 -Chitosan-Berbamine nanocomposites were electrostatically interlocked. The nanocomposites' XRD signals revealed a hexagonal shape. In the DLS spectrum, nanocomposites were found to have a hydrodynamic diameter. As a result of their cytotoxic action, nanocomposites displayed concentration-dependent cytotoxicity. The nanocomposites, like Doxorubicin, caused cell cycle phase arrest in K562 cells. After treatment with IC 50 concentrations of CuO-TiO 2 -Chitosan-Berbamine nanocomposites and Doxorubicin, a substantial percentage of cells were in G2/M stage arrest. Caspase-3, -7, -8, -9, Bax, Bad, Cyt C, and P53 expression were considerably enhanced in K562 cells, whereas Bcl2 expression was decreased, indicating that these cells may have therapeutic potential against human blood cancer/leukemia-derived disorders. As a result, the nanocomposites demonstrated outstanding anticancer potential against leukemic cells. CuO-TiO 2 -Chitosan-Berbamine, according to our findings.

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“…Caspase-3 activity of each sample was calculated according to the standard curve and normalized by the protein concentration. 23 …”

Section: Methodsmentioning

confidence: 99%

Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

Chen

Wang

et al. 2018

Asian J Androl

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Calpain activation contributes to hyperglycemia-induced endothelial dysfunction and apoptosis. This study was designed to investigate the role of calpain inhibition in improving diabetic erectile dysfunction (ED) in mice. Thirty-eight-week-old male C57BL/6J mice were divided into three groups: (1) nondiabetic control group, (2) diabetic mice + vehicle group, and (3) diabetic mice + MDL28170 (an inhibitor of calpain) group. Type 1 diabetes was induced by intraperitoneal injection of streptozotocin at 60 mg kg−1 body weight for 5 consecutive days. Thirteen weeks later, diabetic mice were treated with MDL28170 or vehicle for 4 weeks. The erectile function was assessed by electrical stimulation of the cavernous nerve. Penile tissues were collected for measurement of calpain activity and the endothelial nitric oxide synthase (eNOS)-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway. Terminal deoxynucleotidyl transferase 2'-deoxyuridine 5'-triphosphate nick end labeling (TUNEL) staining was used to evaluate apoptosis. Caspase-3 expression and activity were also measured to determine apoptosis. Our results showed that erectile function was enhanced by MDL28170 treatment in diabetic mice compared with the vehicle diabetic group. No differences in calpain-1 and calpain-2 expressions were observed among the three groups. However, calpain activity was increased in the diabetic group and reduced by MDL28170. The eNOS-NO-cGMP pathway was upregulated by MDL28170 treatment in diabetic mice. Additionally, MDL28170 could attenuate apoptosis and increase the endothelium and smooth muscle levels in corpus cavernosum. Inhibition of calpain could improve erectile function, probably by upregulating the eNOS-NO-cGMP pathway and reducing apoptosis.

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Characterization and cytotoxic activity of apoptosis-inducing pierisin-5 protein from white cabbage butterfly

Cited by 23 publications

References 43 publications

Complete genome of Pieris rapae, a resilient alien, a cabbage pest, and a source of anti-cancer proteins

Complete genome of Pieris rapae, a resilient alien, a cabbage pest, and a source of anti-cancer proteins

[Retracted] CuO‐TiO2‐Chitosan‐Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL‐2 in the Human K562 Cancer Cell Line

Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

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