Characterization and detection of a newly described Asian taeniid using cloned ribosomal DNA fragments and sequence amplification by the polymerase chain reaction

Zarlenga, Dante S.; McManus, Donald P.; Fan, P. C.; Cross, John H.

doi:10.1016/0014-4894(91)90135-j

Cited by 70 publications

(33 citation statements)

References 14 publications

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“…Finally, the complete sequence differences we report here among cox1, cob and nad4 genes of T. asiatica and T. saginata are consistent with the conclusion that T. asiatica is a sister species (Zarlenga et al 1991 ;Zarlenga & George, 1995 ;Galan-Puchades and Mas-Coma, 1996 ;Alkire et al 1998 ;Galan-Puchades and Fuentes, 2000 ;Hoberg et al 2000 ;Schantz, 2000 ;Eom et al 2002 ;Ito et al 2003). This information will add to the knowledge of mitochondrial genomics of parasitic cestodes, provides a resource for the comparative mitochondrial genome analyses of cestodes, and may yield genetic markers for molecular epidemiological investigations into taeniid parasites.…”

supporting

confidence: 92%

Complete sequence and structure of the mitochondrial genome of the human tapeworm,Taenia asiatica(Platyhelminthes; Cestoda)

et al. 2005

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The complete Taenia asiatica mitochondrial genome was amplified by long extension polymerase chain reaction (long PCR) to yield overlapping fragments that were then completely sequenced. The whole mitochondrial genome was 13 703 bp long and contained 12 protein-encoding, 2 ribosomal RNA (small and large subunits), 22 transfer RNA genes and a short non-coding region. Thus, its gene contents are like those typically found in metazoan animal mitochondrial genomes (apart from the absence of atp8). All the genes were transcribed from the same strand. The 3' end 34 bp region of nad4L overlapped with the 5' end portion of nad4. The tRNA genes were 61-69 bp long, and the secondary structures of 18 tRNAs had typical clover-leaf shapes with paired DHU arms. However, trnC, trnS1, trnS2 and trnR had unpaired DHU arms that were 7-12 bp in length. The tRNAs that transferred serine lacked a DHU arm, as is also observed in a number of parasitic platyhelminths and metazoans. However, the trematode trnRs have paired DHU arms. The T. asiatica mtDNA non-coding region was like that in other cestodes since it was composed of a short non-coding region of 72 nucleotides and a long non-coding region of 176 nucleotides separated by a trnL1/, trnS2/, trnL2/, trnR/, nad5 gene cluster. The sequences of the cox1 genes between T. asiatica and T. saginata differ by 4.6%, while the T. asiatica cob gene differs by 4.1% and 12.9% from the cob genes of T. saginata and T. solium, respectively. In conclusion, the T. asiatica mitocondrial genome should provide a resource for comparative mitochondrial genomics and systematic studies of parasitic cestodes.

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confidence: 92%

Complete sequence and structure of the mitochondrial genome of the human tapeworm,Taenia asiatica(Platyhelminthes; Cestoda)

et al. 2005

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“…They concluded that variation in morphology and lack of strongly distinct partitions in genetic sequence data precluded recognition of a species. Interestingly, although it was clear that these taxa could be separated based on an array of morphological and molecular data [22,23,54] and strong evidence for sympatry of T. saginata, T. asiatica and T. solium across Asia was apparent, the former were relegated to subspecies status [24,25].…”

Section: Taenia Asiatica a Valid Speciesmentioning

confidence: 99%

Phylogeny of Taenia: Species definitions and origins of human parasites

Hoberg¹

2006

Parasitology International

128

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Phylogeny is fundamental as it constrains explanations about history and forms our foundation for recognizing and diagnosing species. In the absence of such a framework taxonomists historically relied on intuitive processes, personal judgment and authority, often embracing a typological view of species that disregarded otherwise unequivocal historical and biological criteria. Species of Taenia are among the most characteristic tapeworms infecting carnivores and humans as definitive hosts and indeed Taeniidae is unique among the Eucestoda in requiring 2 obligate mammalian hosts for transmission; a high percentage (> 80%) of life cycles have been completely elucidated among the approximately 45 species and nominal subspecies of Taenia. Until recently there had been no comprehensive attempts at reconstruction of a phylogeny among these important parasites. Such analyses have allowed us to explore the origins and evolution of those independent species of Taenia that occur in humans (T. saginata, T. asiatica, and T. solium) and to understand the ecological and historical processes serving as determinants of biogeography and hostassociation. These studies supported the status of T. asiatica as a valid species and diagnosed a relationship as the sister-species of T. saginata. These conclusions contrasted with a diversity of opinions that would subsume T. asiatica as a subspecies. Recognition of a species constitutes a specific and testable hypothesis, is not an arbitrary decision and is most appropriately assessed in the context of phylogenetic or historical data. Considering macrospecies, a process has been outlined by Brooks and McLennan [Brooks DR, McLennan DA. The nature of diversity: an evolutionary voyage of discovery. University of Chicago Press: Chicago; 2002] as follows: (1) Discovery: a systematist describes the species; (2) Phylogenetic reconstruction; (3) Evaluation I: do sister-species show geographical overlap-are they sympatric or allopatric (use phylogeny + geographical distributions)? (4) Evaluation II: are sister-species reproductively isolated based on information from natural history, ecology and reproductive biology? Species may be viewed in the context of microevolutionary and macroevolutionary processes. For instance, microspecies are defined in ecological time and involve populations and contemporary process that are potentially reversible (reticulate). In contrast, macrospecies as exemplified by T. saginata and T. asiatica are divergent lineages resulting from processes in evolutionary time where an ancestor has undergone a permanent split that is non-reversible (non-reticulate). Applying these criteria in evaluation of T. saginata and T. asiatica, it becomes clear that in evolutionary time these represent historical lineages with independent spatial and temporal trajectories, having separated from a common ancestor near 0.78 to 1.71 MYBP in Africa, or Eurasia. In ecological time, sympatry, reproductive isolation, and differences in life history evident for T. saginata and T. asiatica a...

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“…Fan and his colleagues including Soh from Korea and Kosin from Indonesia expected it to be an undescribed species (Kosin et al, 1972;Soh et al, 1988;Fan et al, 1990). However, as the genetic differences between the two, classical and the new T. saginata were very small, experts from USA and Australia recommended Fan not describing it as a new species (Zarlenga et al, 1991;Bowles and McManus, 1994;McManus and Bowles, 1994;Simanjuntak et al, 1997;Ito et al, , 2004Ito et al, , 2005. Finally, it was described as T. asiatica by other Korean group (Eom and Rim, 1993;Eom, 2006).…”

Section: Outcrossing Of Closely Related Two Species T Saginata and mentioning

confidence: 97%

Recent advances and perspectives in molecular epidemiology of Taenia solium cysticercosis

Itō

Yanagida

Nakao

2016

Infection, Genetics and Evolution

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Characterization and detection of a newly described Asian taeniid using cloned ribosomal DNA fragments and sequence amplification by the polymerase chain reaction

Cited by 70 publications

References 14 publications

Complete sequence and structure of the mitochondrial genome of the human tapeworm,Taenia asiatica(Platyhelminthes; Cestoda)

Complete sequence and structure of the mitochondrial genome of the human tapeworm,Taenia asiatica(Platyhelminthes; Cestoda)

Phylogeny of Taenia: Species definitions and origins of human parasites

Recent advances and perspectives in molecular epidemiology of Taenia solium cysticercosis

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