Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia

Abstract

Previous studies have shown that Vibrio alginolyticus and Vibrio parahaemolyticus can be isolated from similar types of marine samples. In this report, the results of an examination of 567 V. alginolyticus and V. parahaemolyticus strains, isolated from seawater in Jakarta Bay and from more than 30 types of seafood from markets in Jakarta, Indonesia, are presented. Most isolates were from mackerel, shrimp, or squid. Numerical taxonomic analyses clustered 337 isolates and three V. alginolyticus reference strains at S 2 80%. These strains produced acid from sucrose, but only approximately 80% produced acetoin or grew in the presence of 10% NaCl. The frequency of occurrence of V. alginolyticus in seawater samples ranged from 0% (in February and March 1972) to 100% (in September and December 1972) and was highest in seafood samples from August to December 1972. A second cluster of 230 isolates and seven V. parahaemolyticus reference strains was observed at S-82%. These strains did not produce acetoin or acid from sucrose, and approximately 20% grew in the presence of 10% NaCI. V. parahaemolyticus was detected in seawater samples each month, with the highest frequency of occurrence (83.3%) in May 1972. Twenty-nine K antigen serotypes were demonstrated in V. parahaemolyticus isolates, and another 40% were untypable. The modal antibiotic resistance pattern for each species included five drugs. Only 12% of the V. parahaemolyticus strains were Kanagawa positive, and 10% elicited fluid accumulation in ligated rabbit ileal loops. All of the 7 V. alginolyticus strains and 94 (70%) of the V. parahaemolyticus strains tested killed mice when inoculated intraperitoneally. Both V. alginolyticus and V. parahaemolyticus were concluded to be indigenous to seawater and were frequently isolated from seafood in Jakarta, Indonesia. For this reason they should be of concern to clinicians and health authorities in this and other tropical areas.

Occurrence and distribution of Vibrio spp., Listonella spp., and Clostridium botulinum in the Seto Inland Sea of Japan

“…However, the luminescent properties of these strains were greatly reduced, possibly as a result of isolating the strains from TCBS. A consistently higher frequency of occurrence for V. (aginolyticits, as seen in the present study, was reported for seawater and seafood samples collected in various countries (12,30), including Japan (41). This indicates a public health problem, especially for wound infections resulting from exposure to seawater (37).…”

Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia

“…TE-BO-15.2 isolate has a similarity with bacteria V. parahaemolyticus from a marine sponge. According to by (Ulitzur 1974;Molitoris 1985) Vibrio parahaemolyticus and several species occur in estuarine or coastal environment zone of the oceans, mainly as contaminants of different shellfish and absolutely to become dominant species in environments. TE-BO2-01.7, TE-BO2-01.10, TE-BO2-04.2 isolate have a similarity with V. dokdonensis.…”

Jurnal Teknik Lingkungan

“…Vibrio alginolyticus sering ditemukan pada air asin atau pada makhluk perairan seperti ikan (Molitoris et al, 1985). Kehadiran bakteri ini di sampel yang diambil dari reaktor wetland dapat disebabkan oleh kehadiran ikan dalam air limbah yang memasuki IPAL Bojongsoang, atau karena air yang berasal dari peternakan ikan turut masuk ke dalam air tersebut.…”