Characterization and epitope prediction of phosphopyruvate hydratase from <i>Penaeus monodon</i> (black tiger shrimp)

Yang, Yi; Liu, Huiying; Zeng, Wen; Yang, Yunjia; Zhang, Jing; Yin, Jie; Wu, Jie-Ling; Lai, Kefeng

doi:10.1111/1750-3841.15819

Cited by 10 publications

(4 citation statements)

References 25 publications

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“…Recent studies indicate MHC allergenicity in patients with fish allergy [43] and increasing evidence in shellfish allergy. MHC is a potential allergen in P. monodon [44,45] and is cross-reactive with HDM MHC [44]. Studies on banana shrimp (Fenneropenaeus merguiensis) have also found evidence of MHC allergenicity in shrimp-sensitive patients [46].…”

Section: Discussionmentioning

confidence: 99%

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Jerry,

Karnaneedi,

Ruethers

et al. 2024

Foods

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Shellfish allergy affects ~2.5% of the global population and is a type I immune response resulting from exposure to crustacean and/or molluscan proteins. The Australian Redclaw crayfish (Cherax quadricarinatus) is a freshwater species endemic to and farmed in northern Australia and is becoming an aquaculture species of interest globally. Despite being consumed as food, allergenic proteins from redclaw have not been identified or characterised. In addition, as different body parts are often consumed, it is conceivable that redclaw tissues vary in allergenicity depending on tissue type and function. To better understand food-derived allergenicity, this study characterised allergenic proteins in various redclaw body tissues (the tail, claw, and cephalothorax) and how the stability of allergenic proteins was affected through cooking (raw vs. cooked tissues). The potential of redclaw allergens to cross-react and cause IgE-binding in patients allergic to other shellfish (i.e., shrimp) was also investigated. Raw and cooked extracts were prepared from each body part. SDS-PAGE followed by immunoblotting was performed to determine allergen-specific antibody reactivity to sarcoplasmic calcium-binding protein and hemocyanin, as well as to identify redclaw proteins binding to IgE antibodies from individual and pooled sera of shrimp-allergic patients. Liquid chromatography-mass spectrometry (LC/MS) was utilised to identify proteins and to determine the proportion within extracts. Known crustacean allergens were found in all tissues, with a variation in tissue distribution (e.g., higher levels of hemocyanin in the claw and cephalothorax than in the tail). The proportion of some allergens as a percentage of remaining heat-stable proteins increased in cooked tissues. Previously described heat-stable allergens (i.e., hemocyanin and sarcoplasmic calcium-binding protein) were found to be partially heat-labile. Immunoblotting indicated that shrimp-allergic patients cross-react to redclaw allergens. IgE-binding bands, analysed by LC/MS, identified up to 11 known shellfish allergens. The findings of this study provide fundamental knowledge into the diagnostic and therapeutic field of shellfish allergy.

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Section: Discussionmentioning

confidence: 99%

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Jerry,

Karnaneedi,

Ruethers

et al. 2024

Foods

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“…Based on the report of Yang et al, phosphopyruvate hydratase is an enzyme that participates in glycolysis. 35 Aspartate aminotransferase (AAT) is a member of the cluster that is highlighted in the Figure 3. An investigation indicates that AAT participates in glycolysis and plays a role in electron transfer from NADH.…”

Section: Discussionmentioning

confidence: 99%

Introducing Protein Homeostasis and Glycogen Synthesis as Two Targets of Blue Light Radiation in Lentinula edodes

et al. 2022

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Introduction: There are documents about the biological effects of blue light radiation on different organisms. An understanding of the molecular mechanism of radiation effects on biological samples is an important event which has attracted researchers’ attention. Determining the critical dysregulated proteins of Lentinula edodes following blue light radiation is the aim of this study.Methods: 22 differentially expressed proteins of L. edodes in response to 300 lux of blue light were extracted from the related literature. Experimental, text mining and co-expression connections between the queried proteins were assessed via the STRING database. The maps were compared and the critical proteins were identified.Results: Among the 21 queried proteins, six individuals including heat shock HSP70 protein, 20S proteasome subunit, 26S proteasome subunit P45, Aspartate aminotransferase, phosphopyruvate hydratase, and phosphoglucomutase were highlighted as the critical proteins in response to blue light radiation. Conclusion: The finding indicates that protein homeostasis and glycogen synthesis are affected by blue light radiation. Due to the critical roles of proteins as enzymes and structural elements in life maintenance and involvement of glycogen synthesis in energy consumption, blue light radiation can be considered as a life promotional agent in future investigations.

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“…A study showed that eleven (68%) and seven (43%) patients demonstrated IgE-binding activity to titin (Ttn) (identified by mass spectrometry) in raw and heated P. monodon extracts, respectively [29]. Phosphopyruvate hydratase/enolase in P. monodon was identified as a novel, putative shrimp allergen but with a limited number of patients and pediatric patients involved only [73]. Khanaruksombat et al [74] also identified enolase as a potential important allergen in the muscle of P. merguiensis, accompanied with the potential allergen myosin heavy chain (MHC).…”

Section: Other Potential Shrimp Allergensmentioning

confidence: 99%

Genomics of Shrimp Allergens and Beyond

Li,

Chu,

Wai

2023

Genes

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Allergy to shellfishes, including mollusks and crustaceans, is a growing health concern worldwide. Crustacean shellfish is one of the “Big Eight” allergens designated by the U.S. Food and Drug Administration and is the major cause of food-induced anaphylaxis. Shrimp is one of the most consumed crustaceans triggering immunoglobulin E (IgE)-mediated allergic reactions. Over the past decades, the allergen repertoire of shrimp has been unveiled based on conventional immunodetection methods. With the availability of genomic data for penaeid shrimp and other technological advancements like transcriptomic approaches, new shrimp allergens have been identified and directed new insights into their expression levels, cross-reactivity, and functional impact. In this review paper, we summarize the current knowledge on shrimp allergens, as well as allergens from other crustaceans and mollusks. Specific emphasis is put on the genomic information of the shrimp allergens, their protein characteristics, and cross-reactivity among shrimp and other organisms.

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Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp)

Cited by 10 publications

References 25 publications

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Introducing Protein Homeostasis and Glycogen Synthesis as Two Targets of Blue Light Radiation in Lentinula edodes

Genomics of Shrimp Allergens and Beyond

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