Characterization and estimation of sulfaquinoxaline metabolites in animal tissues using liquid chromatography coupled to tandem mass spectrometry

Hoff, Rodrigo Barcellos; Barreto, Fabiano; Melo, Jéssica; Jank, Louíse; Peralba, Maria do Carmo Ruaro; Pizzolato, Tânia Mara

doi:10.1039/c2ay25197c

Cited by 14 publications

(14 citation statements)

References 32 publications

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“…Entretanto, em geral são amostras de fígado ISSN: 2318-356X V.5 N.3 SET/DEZ 2017 de aves. As aves não apresentam um importante mecanismo de metabolização para SQX, que, no entanto, é muito signifcativo para outras espécies (HOFF et al, 2012 Em relação ao exemplo utilizado para avaliar o modelo, podemos concluir que as sulfonamidas disponíveis no mercado veterinário brasileiro têm apresentado perfil mais homogêneo nos últimos anos, focando-se em produtos destinados para suínos e aves. ISSN: 2318-356X V.5 N.3 SET/DEZ 2017 Em síntese, o modelo proposto para priorização será adequado uma vez que os dados necessários sejam disponibilizados: evidenciamos claramente a necessidade urgente de maiores pesquisas e criação de bases de dados sobre o uso de medicamentos veterinários no Brasil.…”

Section: Resultsunclassified

Monitoramento De Resíduos De Medicamentos Veterinários Em Alimentos E No Ambiente: Modelo Para Avaliação

Hoff¹

2018

SCI. ANIM. HEALTH

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O trabalho apresenta uma proposta para elencar prioridades na pesquisa de resíduos de fármacos de uso veterinário utilizados em larga escala na produção animal, tanto em alimentos destinados ao consumo humano como no ambiente. Para exemplificar o modelo proposto, adotou-se neste trabalho o caso específico das sulfonamidas, analisando-se as apresentações farmacêuticas disponíveis no mercado veterinário nacional em dois momentos distintos (2007 e 2017). Segundo o modelo proposto, pode-se dizer que o mesmo é adequado para subsidiar políticas regulatórias e delineamentos de experimentos de métodos de monitoramento de resíduos de medicamentos.

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Section: Resultsunclassified

Monitoramento De Resíduos De Medicamentos Veterinários Em Alimentos E No Ambiente: Modelo Para Avaliação

Hoff¹

2018

SCI. ANIM. HEALTH

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“…The chromatographic separation of SAs was achieved using a modification of the method published elsewhere [33,38]. Some SAs had very similar chromatographic retention time (coeluting), but were well resolved as individual peaks in the MS/MS SRM mode.…”

Section: Post-column Infusion Methodsmentioning

confidence: 99%

Analytical quality assurance in veterinary drug residue analysis methods: Matrix effects determination and monitoring for sulfonamides analysis

Hoff

Rübensam

Jank

et al. 2015

Talanta

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In residue analysis of veterinary drugs in foodstuff, matrix effects are one of the most critical points. This work present a discuss considering approaches used to estimate, minimize and monitoring matrix effects in bioanalytical methods. Qualitative and quantitative methods for estimation of matrix effects such as post-column infusion, slopes ratios analysis, calibration curves (mathematical and statistical analysis) and control chart monitoring are discussed using real data. Matrix effects varying in a wide range depending of the analyte and the sample preparation method: pressurized liquid extraction for liver samples show matrix effects from 15.5 to 59.2% while a ultrasound-assisted extraction provide values from 21.7 to 64.3%. The matrix influence was also evaluated: for sulfamethazine analysis, losses of signal were varying from -37 to -96% for fish and eggs, respectively. Advantages and drawbacks are also discussed considering a workflow for matrix effects assessment proposed and applied to real data from sulfonamides residues analysis.

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“…All data were processed by the software Analyst version 1.4.2 (Applied Biosystems). For parent ion scans, precursor ion scans, and analysis in single reaction monitoring mode (SRM), a liquid chromatograph coupled to a tandem mass spectrometer with electrospray ionization source was used, according to a previously developed and validated method for sulfonamide analysis published elsewhere. , …”

Section: Experimental Sectionmentioning

confidence: 99%

“…In a previous work, we demonstrated the in vivo and in vitro formation of a hydroxylated metabolite, probably mediated by microsomal CYP P450 enzyme complex. This compound, tentatively named hydroxyl-SQX (SQX–OH), shows a very distinct production profile between the species . Equine liver is able to promote hydroxylation of SQX in quantitative proportion while poultry liver demonstrates an absence of that hydroxylation activity.…”

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Structural Elucidation of Sulfaquinoxaline Metabolism Products and Their Occurrence in Biological Samples Using High-Resolution Orbitrap Mass Spectrometry

Hoff

Meneghini²,

Pizzolato

et al. 2014

Anal. Chem.

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Four previously unreported metabolism products of sulfaquinoxaline (SQX), a widely used veterinary medicine, were isolated and analyzed using liquid chromatography coupled to high-resolution Orbitrap mass spectrometry. Metabolites were structurally elucidated, and a fragmentation pathway was proposed. The combination of high-resolution MS(2) spectra, linear ion trap MS(2), in-source collision-induced dissociation (CID) fragmentation, and photolysis were used to analyze SQX and its metabolites. All metabolism products identified showed a similar fragmentation pattern to that of the original drug. Differential product ions were produced at m/z 162 and 253 which contain the radical moiety with more 16 Da units than sulfaquinoxaline. This occurs by a hydroxyl attachment to the quinoxaline moiety. With the exception of two low-intensity compounds, all the mass errors were below 5.0 ppm. The distribution of these metabolites in some animal species are also presented and discussed.

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Characterization and estimation of sulfaquinoxaline metabolites in animal tissues using liquid chromatography coupled to tandem mass spectrometry

Cited by 14 publications

References 32 publications

Monitoramento De Resíduos De Medicamentos Veterinários Em Alimentos E No Ambiente: Modelo Para Avaliação

Monitoramento De Resíduos De Medicamentos Veterinários Em Alimentos E No Ambiente: Modelo Para Avaliação

Analytical quality assurance in veterinary drug residue analysis methods: Matrix effects determination and monitoring for sulfonamides analysis

Structural Elucidation of Sulfaquinoxaline Metabolism Products and Their Occurrence in Biological Samples Using High-Resolution Orbitrap Mass Spectrometry

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