Characterization and expression analysis of the Arabidopsis mir169 family

Li, Yanjie; Fu, Yaru; Ji, Lusha; Wu, Changai; Zheng, Chengchao

doi:10.1016/j.plantsci.2010.01.007

Cited by 46 publications

(49 citation statements)

References 43 publications

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“…Our data suggest a predominant localization of miR169i/j/k at the vasculature level, as also evidenced by Li et al 42 in Arabidopsis and support the already hypothesized role of miR169 family members as novel candidates for long-distance signaling of the N status in the plant system. 30, 42 An analog function could be hypothesized for miR166j/k/n which evidenced a similar distribution among tissues, even if it displayed a different regulation of expression in response to the nitrate supply. The importance of these two families of miRNAs for vascular tissue differentiation in response to nitrogen was also supported by the results obtained from both the miRNA's target prediction and the analysis of cis-elements in their promoter.…”

Section: Maize Nitrate-responsive Mirnassupporting

confidence: 91%

The miRNA-mediated post-transcriptional regulation of maize response to nitrate

Trevisan

Begheldo

Nonis

et al. 2012

Plant Signaling & Behavior

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Section: Maize Nitrate-responsive Mirnassupporting

confidence: 91%

The miRNA-mediated post-transcriptional regulation of maize response to nitrate

Trevisan

Begheldo

Nonis

et al. 2012

Plant Signaling & Behavior

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“…Some miRNA family numbers in tobacco are larger than the miRNA numbers reported for the same families in different plant species. miR169, with 14 members, is the largest miRNA family in Arabidopsis and has been shown to be expressed mainly in young seedlings as opposed to mature tissues (Li et al 2010). miR156, with seven members, is also one of the more predominant miRNA families in Arabidopsis (Griffiths-Jones et al 2008) and has been shown to function in developmental timing, as well as the phase change from vegetative to reproductive growth (Wu and Poethig 2006).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of microRNAs and their target genes in tobacco (Nicotiana tabacum)

Frazier

Xie

Freistaedter

et al. 2010

Planta

115

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microRNAs (miRNAs) are a recently discovered class of small (~21 nt) endogenous gene regulators that have been shown to play an important role in plant growth and development by aiding in organ maturation, hormone signaling, tissue differentiation, and plant tolerance to environmental stress. Since a list of miRNAs has never been generated for tobacco, we employed genome survey sequence analysis to computationally identify 259 potentially conserved tobacco miRNAs, belonging to 65 families, and validated 11 of these miRNAs using qRT-PCR. The 65 miRNA families were dramatically different in size. miRNA precursor (pre-miRNA) sequence analysis showed that tobacco pre-miRNAs greatly varied from 45 to 635 nt in length with an average of 141 ± 108 nt. We were also able to determine the presence of antisense miRNAs as well as miRNA clusters in tobacco. Using previously established protocols, a total of 1,225 potential target genes were predicted for the newly identified tobacco miRNAs. These target genes include transcription factors, DNA replication proteins, metabolic enzymes, as well as other gene targets necessary for proper plant maturation. The results of this study show that conserved miRNAs exist in tobacco and suggest that these miRNAs may play an important role in tobacco growth and development.

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“…MiR169 is a large miRNA family that is widespread in the plant kingdom (Li et al 2010), and plays important roles in plant development and responses to environmental cues (Combier et al 2006). In rice (Oryza sativa), some members of the miR169 family were induced by drought (Zhao et al 2007) and salt stress (Zhao et al 2009).…”

mentioning

confidence: 99%

Over-expression of microRNA169 confers enhanced drought tolerance to tomato

et al. 2010

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Plant miRNA regulates multiple developmental and physiological processes, including drought responses. We found that the accumulation of Sly-miR169 in tomato (Solanum lycopersicum) was induced by drought stress. Consequently, Sly-miR169 targets, namely, three nuclear factor Y subunit genes (SlNF-YA1/2/3) and one multidrug resistance-associated protein gene (SlMRP1), were significantly down-regulated by drought stress. Constitutive over-expression of a miR169 family member, Sly-miR169c, in tomato plant can efficiently down-regulate the transcripts of the target genes. Compared with non-transgenic plants, transgenic plants over-expressing Sly-miR169c displayed reduced stomatal opening, decreased transpiration rate, lowered leaf water loss, and enhanced drought tolerance. Our study is the first to provide evidence that the Sly-miR169c negatively regulates stomatal movement in tomato drought responses.

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Characterization and expression analysis of the Arabidopsis mir169 family

Cited by 46 publications

References 43 publications

The miRNA-mediated post-transcriptional regulation of maize response to nitrate

The miRNA-mediated post-transcriptional regulation of maize response to nitrate

Identification and characterization of microRNAs and their target genes in tobacco (Nicotiana tabacum)

Over-expression of microRNA169 confers enhanced drought tolerance to tomato

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