Characterization and expression analysis of Cyclin-dependent kinase 7 gene and protein in ovaries of the giant tiger shrimp Penaeus monodon

Phinyo, Mahattanee; Nounurai, Pachumporn; Hiransuchalert, Rachanimuk; Jarayabhand, Padermsak; Klinbunga, Sirawut

doi:10.1016/j.aquaculture.2014.05.022

Cited by 22 publications

(30 citation statements)

References 45 publications

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“…In view of the important role that such complexes play in ovarian development, and the reduced reproductive performance observed in aquacultures of black tiger shrimp, we intended to study the interaction between cyclin E and CDK2 in this organism. Research into cell cycle regulation may improve our knowledge of the molecular mechanisms controlling development and maturation of ovaries and oocytes in P. monodon (Phinyo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Despite the important role played by cyclin E/CDK2 in the cell cycle, the functional characterization of this complex has predominately been focused on vertebrate species, particularly mammals (Sheaff et al, 1997;Kolupaeva and Basilico, 2012;Duong et al, 2013). In recent years, increasing attention has been paid to the activity of cyclin/CDK complexes in invertebrates, especially shellfish such as Scylla paramamosain (Zieba et al, 2012;Huang et al, 2013), the black tiger shrimp Penaeus monodon (Phinyo et al, 2014), and the thunberg Crassostrea gigas (Wang et al, 2011). However, compared to the progress made in studying the functional mechanism of the cyclin E/CDK2 complex in vertebrates, related research in invertebrates, particularly shrimp, is lacking.…”

Section: Introductionmentioning

confidence: 99%

“…However, reduced reproductive performance in aquacultures of black tiger shrimp has caused economic losses and hampered the fishing industry (Wen et al, 2015). In general, unilateral eyestalk ablation is used to induce P. monodon ovarian maturation, but this technique can lead to the death of parent shrimp and lower spawning quality (Benzie, 1998;Wongprasert et al, 2006;Phinyo et al, 2014). Therefore, the examination of alternative approaches is imperative.…”

Section: Introductionmentioning

confidence: 99%

“…Mitosis and meiotic maturation of animal oocytes is regulated by the cyclin/CDK complex (Lilly et al, 2000;Nguyen et al, 2002;Ortega et al, 2003;Phinyo et al, 2014). Therefore, functional analysis of this complex would improve our understanding of ovary development.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

Zhao

Qiu

2016

Genet. Mol. Res.

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ABSTRACT. Reduced reproductive performance of the black tiger shrimp (Penaeus monodon) has caused economic losses and hampered the fishing industry. Detailed investigation of the molecular mechanism by which the cell cycle is regulated in this organism is needed to understand the development and maturation of ovaries and oocytes, with a view to improving reproductive capacity. Cell cycle progression is mainly determined by cyclin-dependent kinase (CDK) and cyclin complexes, the cyclin E/CDK2 complex playing a key role in G1/S transition. However, knowledge of the interplay between cyclin E and CDK2 in invertebrates remains limited. In this study, full-length P. monodon cyclin E (Pmcyclin E) and CDK2 (PmCDK2) sequences were cloned. The open reading frame of Pmcyclin E was 1263 bp in 2 C. Zhao et al. Genetics and Molecular Research 15 (3): gmr.15038716 length and encoded a 47.9-kDa protein, while that of PmCDK2 was 921 bp, encoding a protein of 34.9 kDa. Recombinant cyclin E and CDK2 proteins were expressed in Escherichia coli and purified by Ni-chelating affinity chromatography. In addition, a pull-down assay was performed to identify any interaction between Pmcyclin E and PmCDK2. This research provides a basis for the study of the functional mechanisms of the cyclin E/CDK2 complex in shrimp, further enriching our knowledge of invertebrate cell cycle regulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

Zhao

Qiu

2016

Genet. Mol. Res.

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“…Research on the cell cycle regulation can improve the knowledge of molecular mechanisms controlling the development and maturation of ovaries/oocytes in P. monodon (Phinyo et al, 2014). To date, the regulation of oocyte maturation in crustaceans is poorly understood as compared to that in other oviparous animals.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)

Wang

Zhao

et al. 2016

Genet. Mol. Res.

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ABSTRACT. MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) observed in ovaries. In the ovaries, the PmMAT1 gene was continuously but differentially expressed during the maturation stages. Comparative analyses of MAT1, CDK7, and cyclin H in the CAK complex of P. monodon indicated that the expression of CDK7 and cyclin H coincided with that of MAT1 during the ovary maturation stages. Serotonin (5-HT) injection promoted the expression level of PmMAT1 in the ovaries of shrimp at 6-48 h post-injection. These results indicate that PmMat1 plays a prominent role in the process of ovarian maturation.

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Characterization, expression and silencing by RNAi of p53 from Penaeus monodon

et al. 2016

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The tumor suppressor p53 is a sequence-specific transcription factor, whose target genes can regulate genomic stability, the cellular response to DNA damage and cell-cycle progression. In the present study, the full-length complementary DNA (cDNA) sequence of p53 gene from Penaeus monodon (Pmp53) was cloned by the technology of rapid amplification of cDNA ends (RACE). The cDNA of Pmp53 was 2239 bp, encoding a protein of 450 amino acids with calculated molecular weight of 50.62 kDa. The temporal expression of Pmp53 in different tissues (ovary, heart, intestine, brain, muscles, stomach and gills) and different developmental stages of ovary was investigated by real-time quantitative PCR (RT-qPCR). The lowest expression level of Pmp53 was observed in the stomach, while the highest expression level was detected in the brain. During the ovary development stages, the expression level of Pmp53 reached the peak at stage III. RNA interference (RNAi) and serotonin (5-hydroxytryptamine, 5-HT) injection experiments were conducted to study the expression profile of Pmp53 and PmCDK2 (cyclin-dependent kinase 2, CDK2). Knocked down of Pmp53 by dsRNA-p53 was sequence-specific and successful. Expression levels of Pmp53 and PmCDK2 in ovary of P. monodon were significantly increased at 12-96 h post 5-HT injection. These results indicate that Pmp53 may be involved in the regulation of ovarian development of P. monodon.

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Characterization and expression analysis of Cyclin-dependent kinase 7 gene and protein in ovaries of the giant tiger shrimp Penaeus monodon

Cited by 22 publications

References 45 publications

Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)

Characterization, expression and silencing by RNAi of p53 from Penaeus monodon

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