Characterization and expression of the CXCR1 and CXCR4 in miiuy croaker and evolutionary analysis shows the strong positive selection pressures imposed in mammal CXCR1

The CXC chemokine receptors of fish: Insights into CXCR evolution in the vertebrates

“…Subsequently CXCR1 and CXCR2 have been found in a wide range of teleost fish species including rainbow trout (Oncorhynchus mykiss) (Xu et al, 2014a;Zhang et al, 2002), fugu (Takifugu rubripes) (Huising et al, 2003a,b); zebrafish (Danio rerio) (Deng et al, 2013;Oehlers et al, 2010); Chinese perch (Siniperca chuatsi) , miiuy croaker (Miichthys miiuy) (Xu et al, 2014b) and several elasmobranch species, including lesser spotted catshark (Scyliorhinus caniculus), basking shark (Cetorhinus maximus), great white shark (Carcharodon carcharias), cuckoo ray (Raja naevus) (Goostrey et al, 2005) and elephant shark (Callorhinchus milii) (Venkatesh et al, 2014), and more recently in the coelacanth (Latimeria chalumnae) (Xu et al, 2014a) (STTIL[I]) in the C-terminal region, which have been shown to be important for cellular signalling of human CXCR2 (Fig. 2) (Baugher and Richmond, 2008;Fan et al, 2001;Marchese, 2014).…”

Comparative genomic of the teleost cathepsin B and H and involvement in bacterial induced immunity of miiuy croaker

“…Primers used for qRT-PCR detection of CTSB and CTSH (CTSB-F/R and CTSH-F/R) genes and the primers of b-actin (b-actin-F/R) used as internal control were listed in Table S2. The qRT-PCR reactions were carried out as described by Xu et al [33]. Dissociation curve analysis was performed after each assay to determine target specificity.…”

Sequence and expression analysis of rainbow trout CXCR2, CXCR3a and CXCR3b aids interpretation of lineage-specific conversion, loss and expansion of these receptors during vertebrate evolution

“…However, CXCR1/R2 like genes have been described from several fish species including common carp Cyprinus carpio (Fujiki et al, 1999), rainbow trout Oncorhynchus mykiss (Zhang et al, 2002), fugu Fugu rubripes (Saha et al, 2007), mandarin fish Siniperca chuatsi (Chen et al, 2009) zebrafish Danio rerio (Oehlers et al, 2010) and miiuy croaker Miichthys miiuy (Xu et al, 2013). CXCR3 has also been reported in grass carp Ctenopharyngodon idella (Chang et al, 2007) and medaka Oryzias latipes (Aghaallaei et al, 2010).…”