Characterization and expression patterns of wnt4 in Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) during embryonic and larval development

Abstract

Wnt4 (Wingless-type MMTV integration site family member 4) has been demonstrated to play critical roles in a wide variety of biological processes, including embryonic development, cell proliferation, and differentiation in vertebrates, but its function in crustaceans is still not clear. In the present study, the full-length wnt4 cDNA sequence was cloned and characterized for the ridgetail white prawn Exopalaemon carinicauda. The expression patterns of the wnt4 mRNA in embryos and larvae at different stages were investigated. The tissue distribution showed that wnt4 was obviously expressed in eyestalk and hepatopancreas. During embryonic development, the wnt4 was highly expressed in all developmental stages except the zygote, two-cell stage, and late zoaea stage. The wnt4 mRNA was expressed in Z1-Z5 and post-larval stages. Taken together, the present study indicates that the wnt4 gene may be involved in the regulation of embryonic and larval development in the ridgetail white prawn.

Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

“…The ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) [currently also again referred to as Palaemon carinicauda Holthuis, 1950], is one of the most important economic crustacean species in China because of its fast growth, strong adaptability, delicious taste and rich nutritional value (Wang et al, 2021;Lai et al, 2022b;Zhang et al, 2022). Many studies have been carried out on the shrimp model with regard to its breeding and aquaculture (Wang et al, 2021;Lai et al, 2022a;Pang et al, 2022), and its physiology, biochemistry, and gene functioning (Gao et al, 2017(Gao et al, , 2021Ma et al, 2020;Shen et al, 2021;Lai et al, 2022b).…”