Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06

Chung, Chi-Ming; Jamann, Tiffany M.; Longfellow, Joy; Nelson, Rebecca

doi:10.1007/s00122-010-1303-z

Cited by 64 publications

(81 citation statements)

References 90 publications

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“…Marker analysis showed that all Htn1 introgression lines carry the same haplotype in the target interval (SI Appendix, Table S5). The NCLB resistance gene Ht2 also maps on maize chromosome 8 to a similar interval to that of Htn1 (16,(20)(21)(22)(23). We amplified the ZmWAK-RLK1 homolog from the Ht2 introgression line B37Ht2.…”

Section: Zmwak-rlp1mentioning

confidence: 99%

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The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Hurni

Scheuermann

Krattinger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

277

221

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Northern corn leaf blight (NCLB) caused by the hemibiotrophic fungus Exserohilum turcicum is an important foliar disease of maize that is mainly controlled by growing resistant maize cultivars. The Htn1 locus confers quantitative and partial NCLB resistance by delaying the onset of lesion formation. Htn1 represents an important source of genetic resistance that was originally introduced from a Mexican landrace into modern maize breeding lines in the 1970s. Using a high-resolution map-based cloning approach, we delimited Htn1 to a 131.7-kb physical interval on chromosome 8 that contained three candidate genes encoding two wall-associated receptor-like kinases (ZmWAK-RLK1 and ZmWAK-RLK2) and one wall-associated receptor-like protein (ZmWAK-RLP1). TILLING (targeting induced local lesions in genomes) mutants in ZmWAK-RLK1 were more susceptible to NCLB than wild-type plants, both in greenhouse experiments and in the field. ZmWAK-RLK1 contains a nonarginine-aspartate (non-RD) kinase domain, typically found in plant innate immune receptors. Sequence comparison showed that the extracellular domain of ZmWAK-RLK1 is highly diverse between different maize genotypes. Furthermore, an alternative splice variant resulting in a truncated protein was present at higher frequency in the susceptible parents of the mapping populations compared with in the resistant parents. Hence, the quantitative Htn1 disease resistance in maize is encoded by an unusual innate immune receptor with an extracellular wall-associated kinase domain. These results further highlight the importance of this protein family in resistance to adapted pathogens.wall-associated receptor-like kinase | quantitative disease resistance | pattern recognition receptor | Exserohilum turcicum | maize

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Section: Zmwak-rlp1mentioning

confidence: 99%

“…The strength of the Htn1 resistance depends on environmental conditions and maize genotype (19). Htn1 has been mapped to maize chromosome 8 ∼10 cM distal to the NCLB resistance gene Ht2 (20)(21)(22)(23).…”

mentioning

confidence: 99%

The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Hurni

Scheuermann

Krattinger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

277

221

View full text Add to dashboard Cite

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“…Diseased leaf area (DLA) was rated on a per row basis three times after flowering at an interval of 7-10 days, using a percentage scale of 0-100 with increments of 1, where 0 indicates a plant with no disease and 100 indicates a completely diseased plant. Area under the disease progress curve (AUDPC) was calculated as described previously (Chung et al 2010a). …”

Section: Plant Materialsmentioning

confidence: 99%

“…The resulting DNA was diluted 1:100 with water for KASPar (LGC Genomics, Hoddesdon, Herfordshire, UK) genotyping. CTAB DNA extractions were performed using 0.1 mg of fresh tissue as described previously (Doyle and Dickson 1987;Chung et al 2010a). …”

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confidence: 99%

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

et al. 2014

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Multiple disease resistance has important implications for plant fitness, given the selection pressure that many pathogens exert directly on natural plant populations and indirectly via crop improvement programs. Evidence of a locus conditioning resistance to multiple pathogens was found in bin 1.06 of the maize genome with the allele from inbred line "Tx303" conditioning quantitative resistance to northern leaf blight (NLB) and qualitative resistance to Stewart's wilt. To dissect the genetic basis of resistance in this region and to refine candidate gene hypotheses, we mapped resistance to the two diseases. Both resistance phenotypes were localized to overlapping regions, with the Stewart's wilt interval refined to a 95.9-kb segment containing three genes and the NLB interval to a 3.60-Mb segment containing 117 genes. Regions of the introgression showed little to no recombination, suggesting structural differences between the inbred lines Tx303 and "B73," the parents of the fine-mapping population. We examined copy number variation across the region using next-generation sequencing data, and found large variation in read depth in Tx303 across the region relative to the reference genome of B73. In the fine-mapping region, association mapping for NLB implicated candidate genes, including a putative zinc finger and pan1. We tested mutant alleles and found that pan1 is a susceptibility gene for NLB and Stewart's wilt. Our data strongly suggest that structural variation plays an important role in resistance conditioned by this region, and pan1, a gene conditioning susceptibility for NLB, may underlie the QTL.T HE genes and loci that influence host-pathogen interactions vary in allele effects, specificities, and linkage relationships. While disease resistance can be conditioned by single genes with large effect (Bent 1996;Jones and Dangl 2006), the emerging model of resistance for many plant diseases is complex in nature, with many genes and loci functioning in concert and each contributing a small proportion of the total phenotypic variation Poland et al. 2011;Cook et al. 2012b). Each locus has a unique profile, with some loci contributing broadspectrum protection against diverse pathogen species and strains. Investigating these intricacies offers the opportunity to understand the diverse ways in which plants defend themselves against microbial assault.Correlated responses to multiple diseases have been observed in various germplasm panels, implying that there are loci and genes that condition broad-spectrum resistance (Rossi et al. 2006;Gurung et al. 2009;Wisser et al. 2011). At the chromosomal segment level, disease and insect resistance loci colocalize in a nonrandom fashion (McMullen and Simcox 1995;Williams 2003;Wisser et al. 2005) and loci have been identified that confer resistance to diverse pathogen isolates and taxa (Zwonitzer et al. 2010;Chung et al. 2011;Belcher et al. 2012). There is evidence to suggest that gene clusters can confer resistance to more than one disease. A cluster of germin-like ...

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“…In the present study, we used QTLs on these chromosomes, as well as a QTL on chromosome 3 (Ogliari et al, 2007) and a QTL on chromosome 8 (Chung et al, 2010a;2010b;Wang et al, 2012).…”

Section: Association Mapping For the Resistance To Northern Leaf Blightmentioning

confidence: 99%

Association mapping and genetic control for northern leaf blight (Exserohilum turcicum) resistance in maize lines

Kaefer¹,

Schuelter²,

Schuster³

et al. 2017

Aust J Crop Sci

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The aim of this work was to identify genomic regions associated with northern leaf blight resistance (Exserohilum turcicum) in common maize lines and to study the control involved in the resistance. For association mapping, 72 maize lines were previously genotyped for SNP markers on the 650K platform (Affymetrix®), and their respective genotypic values were predicted by mixed models for northern leaf blight. In order to avoid spurious associations between SNP markers and the studied character, the analysis of population structure was initially performed. The analysis of association between the SNP and northern leaf blight markers was carried out using a linear mixed model. For the study of genetic control, the experiment was conducted in 2016 and it was composed of randomized blocks with three repetitions. Through the notes attributed to the lines and their respective generations, the genotypic data related to the inheritance of the studied disease were extracted. Analyzing the results, QTLs were found on chromosomes 1, 2, 3, 4, 6, 8, 9 and 10 for northern leaf blight in maize, which may increase, reduce or even override the effect of this attribute. Among the QTLs found, five genomic regions were detected for increased resistance to northern leaf blight with the use of SNP markers, found in chromosomes 3 (SNP210703), 8 (SNP507268 and SNP507269), 9 (SNP544616) and 10 (SNP610500). The genetic control of resistance to northern leaf blight is quantitative, with the additive effects being more important in the character determination. In addition, it presents high restricted heritability (88%), which allows good selection efficiency and selection gains.

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Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06

Cited by 64 publications

References 90 publications

The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

Association mapping and genetic control for northern leaf blight (Exserohilum turcicum) resistance in maize lines

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