Characterization and Functional Analysis of CReMM, a Novel Chromodomain Helicase DNA-binding Protein

Shur, Irena; Benayahu, Dafna

doi:10.1016/j.jmb.2005.06.049

Cited by 48 publications

(81 citation statements)

References 32 publications

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“…Since the discovery of murine Chd1 in 1993, other Chd genes have been identified, yielding a total of 9 highly conserved genes from diverse organisms [9,10,39,40,51]. The Chd family is divided into three subfamilies based on structural and sequence similarities [9,10,32,39,40].…”

Section: Discussionmentioning

confidence: 99%

“…3). It should be noted that Chd9 is also referred to as CReMM (chromatin-related mesenchymal modulator) [39]. This subfamily is defined by additional functional motifs in the C-terminal region, including paired BRK (Brahma and Kismet) domains, a SANT-like (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIIB) domain, CR domains, and a DNAbinding domain, [39][40][41].…”

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

“…Biochemical analyses determined that SANT domains, which interact primarily with unmodified histone tails, are unique histone-interaction modules that couple histone binding to enzyme catalysis [4,6]. To date, the CR domains, CR1-CR3, are moieties of unknown function that are only found in Chd9 [39,40]. As with the Chd1-Chd2 subfamily, the DNA-binding domain of the third subfamily also shows a preference for binding to AT-rich tracts.…”

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

“…As with the Chd1-Chd2 subfamily, the DNA-binding domain of the third subfamily also shows a preference for binding to AT-rich tracts. However, DNA-binding activity has only been demonstrated experimentally for Chd9 to date [39].…”

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

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The Chd family of chromatin remodelers

Marfella¹,

Imbalzano²

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

351

369

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Chromatin remodeling enzymes contribute to the dynamic changes that occur in chromatin structure during cellular processes such as transcription, recombination, repair, and replication. Members of the chromodomain helicase DNA-binding (Chd) family of enzymes belong to the SNF2 superfamily of ATP-dependent chromatin remodelers. The Chd proteins are distinguished by the presence of two N-terminal chromodomains that function as interaction surfaces for a variety of chromatin components. Genetic, biochemical, and structural studies demonstrate that Chd proteins are important regulators of transcription and play critical roles during developmental processes. Numerous Chd proteins are also implicated in human disease.

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Section: Discussionmentioning

confidence: 99%

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

Section: Chd5-chd9 Subfamilymentioning

confidence: 99%

See 2 more Smart Citations

The Chd family of chromatin remodelers

Marfella¹,

Imbalzano²

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

351

369

View full text Add to dashboard Cite

show abstract

“…Induction of myogenic cell fates requires the recruitment by CHD2 of a chromatin destabilizing histone variant, histone H3.3, to muscle differentiation genes, which then facilitates binding and gene activation by the transcription factor MyoD [39]. Interestingly, CHD9, also known as chromatin-related mesenchymal modulator, binds to and promotes the expression of osteocalcin (bone gamma-carboxyglutamate), one of the major genes responsible for promoting bone development [40,41]. Collectively, these observations emphasize the importance of CHD family proteins in mesenchymal cell fate decisions and highlight the basic principle that these proteins can have diverse and potentially nonredundant functions within the same stem cell type.…”

Section: Chd Proteins and Mesenchymal Stem Cellsmentioning

confidence: 99%

Chromodomain Helicase DNA-Binding Proteins in Stem Cells and Human Developmental Diseases

Micucci

Sperry

Martin

2015

Stem Cells and Development

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Genotranscriptomic meta‐analysis of the CHD family chromatin remodelers in human cancers – initial evidence of an oncogenic role for CHD7

et al. 2017

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Chromodomain helicase DNA binding proteins (CHDs) are characterized by N‐terminal tandem chromodomains and a central adenosine triphosphate‐dependent helicase domain. CHDs govern the cellular machinery's access to DNA, thereby playing critical roles in various cellular processes including transcription, proliferation, and DNA damage repair. Accumulating evidence demonstrates that mutation and dysregulation of CHDs are implicated in the pathogenesis of developmental disorders and cancer. However, we know little about genomic and transcriptomic alterations and the clinical significance of most CHDs in human cancer. We used TCGA and METABRIC datasets to perform integrated genomic and transcriptomic analyses of nine CHD genes in more than 10 000 primary cancer specimens from 32 tumor types, focusing on breast cancers. We identified associations among recurrent copy number alteration, gene expression, clinicopathological features, and patient survival. We found that CHD7 was the most commonly gained/amplified and mutated, whereas CHD3 was the most deleted across the majority of tumor types, including breast cancer. Overexpression of CHD7 was more prevalent in aggressive subtypes of breast cancer and was significantly correlated with high tumor grade and poor prognosis. CHD7 is required to maintain open, accessible chromatin, thus providing fine‐tuning of transcriptional regulation of certain classes of genes. We found that CHD7 expression was positively correlated with a small subset of classical oncogenes, notably NRAS, in breast cancer. Knockdown of CHD7 inhibits cell proliferation and decreases gene expression of several CHD7 targets, including NRAS, in breast cancer cell lines. Thus, our results demonstrate the oncogenic potential of CHD7 and its association with poor prognostic parameters in human cancer.

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Characterization and Functional Analysis of CReMM, a Novel Chromodomain Helicase DNA-binding Protein

Cited by 48 publications

References 32 publications

The Chd family of chromatin remodelers

The Chd family of chromatin remodelers

Chromodomain Helicase DNA-Binding Proteins in Stem Cells and Human Developmental Diseases

Genotranscriptomic meta‐analysis of the CHD family chromatin remodelers in human cancers – initial evidence of an oncogenic role for CHD7

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