Characterization and gene mapping of a chlorophyll-deficient mutant clm1 of Triticum monococcum L.

Ansari, Mohammad Javed; Al-Ghamdi, Ahmad; Kumar, Rahul; Usmani, Salma; Alattal, Yehya; Adgaba, Nuru; Mohamed, Abdellatif A.; Singh, Kuldeep; Dhaliwal, H. S.

doi:10.1007/s10535-013-0307-3

Cited by 30 publications

(21 citation statements)

References 42 publications

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“…EMS can form adducts with nucleotides, causing them to mispair with their complementary bases and introducing base changes after replication [15]. EMS-induced mutants have been created in rice [16], maize [17], barley [18], diploid wheat [19,20], and hexaploid wheat [21,22]. Some genes, such as klu, regulating seed size [23], and als3-1, required by seedlings for growth in aluminum-toxic environments [24], have been isolated using EMS-induced mutations in Arabidopsis thaliana.…”

Section: Introductionmentioning

confidence: 99%

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

Zhang¹,

Wang²,

Guo³

et al. 2015

The Crop Journal

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Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate (EMS). F 2 and F 2:3 generations produced from crosses of M8008 × YN15 (MY) and M8008 × SJZ54 (MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height (PH), spike length (SL), fertile spikelet number per spike (FSS), grain width (GW), grain length (GL), GL/GW ratio (GLW), and thousand-grain weight (TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat (SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci (QTLs) forming QTL clusters (one in MY and two in MS) were detected. The QTL clusters coinciding with (MY population) or near (MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density (FFD), and TGW and to increases in GLW. The cluster in the wmc25-barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant (SN), and SL.

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Section: Introductionmentioning

confidence: 99%

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

Zhang¹,

Wang²,

Guo³

et al. 2015

The Crop Journal

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“…Artificially induced mutations are produced by various approaches. Many of them are induced with chemicals (Ansari et al, ), radiation (Chen et al, ) and biological agents (Bang et al, ). The frequency of artificially induced mutations is ≤ 3% higher than that of spontaneous mutations.…”

Section: Discussionmentioning

confidence: 99%

Examination and genetic analysis of a yellow‐green leaf mutant a269 of sweetpotato

et al. 2019

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The genetic behaviour of a yellow-green leaf sweetpotato mutant, a269, was analysed to prepare for genetic mapping and cloning of this mutant and to elucidate the regulation of its molecular mechanisms. The phenotypic stability of the mutant was observed under natural conditions, and photosynthetic pigments in mature leaves were measured. GN1323 and a269 were crossed and backcrossed. Genetic behaviour was assessed using phenotypic expression and segregation in F 1 , BC 1 and BC 2 . The a269 leaves were yellow-green at all stages after germination. The thylakoid lamellae of a269 were disorganized, had incomplete grana and were fewer compared with GN1323. The direct and reciprocal F 1 populations had normal leaf colour, whereas the BC 2 populations had yellow-green leaves. Segregation of plants with normal-and yellow-green leaf colour in the four BC 1 populations satisfied the backcross segregation ratio of 11 : 1 for auto-allohexaploids. The a269 mutant is stable, and its trait is controlled by one recessive nuclear locus. K E Y W O R D S auto-allohexaploid, genetic analysis, genetic mapping, photosynthetic pigment, sweetpotato, yellow-green leaf mutant | 387 LUO et aL. performed the experiments. O RCI D Zhongxia Luohttps://orcid.org/0000-0001-9485-8769

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“…These studies identified that most of the leaf color mutants are controlled by a single recessive nuclear gene. For example, many leaf mutants of soybean[33], wheat [36], and rice [37] were all controlled by a single recessive nuclear gene. In addition, there are a few leaf color mutants, in which the traits are controlled by the cytoplasmic gene.…”

Section: Discussionmentioning

confidence: 99%

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Gao

Sang

Chen

et al. 2018

Preprint

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11Leaf color mutation in sesame always affects the growth and development of plantlets, and their 12 yield. To clarify the mechanisms underlying leaf color regulation in sesame, we analyzed a 13 yellow-green leaf mutant. Genetic analysis of the mutant selfing revealed 3 phenotypes-YY, 14 light-yellow (lethal); Yy, yellow-green; and yy, normal green-controlled by an incompletely 15 dominant nuclear gene, Siyl-1. In YY and Yy, the number and morphological structure of the 16 chloroplast changed evidently, with disordered inner matter, and significantly decreased 17 chlorophyll content. To explore the regulation mechanism of leaf color mutation, the proteins 18 expressed among YY, Yy, and yy were analyzed. All 98 differentially expressed proteins (DEPs) 19 were classified into 5 functional groups, in which photosynthesis and energy metabolism 20 (82.7%) occupied a dominant position. Our findings provide the basis for further molecular 21 mechanism and biochemical effect analysis of yellow leaf mutants in plants. 22 24which also contain numerous beneficial minerals, antioxidants, and multi-vitamins [1]. 25Compared with other oilseed crops, sesame is still a low yield crop with low harvest index. 26The key research objectives in sesame are to increase the photosynthesis efficiency and the 27 yield per square area. 28Leaf color is an important trait related with the chlorophyll content, and always affects the 29 photosynthesis efficiency and the final productivity [2]. In plants, the chloroplast structure 30 always alters the composition and content of photosynthetic pigments. Several studies on the 31 leaf color mutants have uncovered a wide range of leaf color mutation types [3]. Most 32 mutagenesis of leaf color were related to the structure and function of the chloroplast [4], 33 chlorophyll biosynthesis and degradation mechanisms [5], photosynthesis [6], chloroplast 34 developmental characteristics [7], genetics [8], and molecular mechanisms [9]. Furthermore, 35some mutants were used for crop breeding to higher biomass, quality, and/or other specific 36 characteristics [10]. There is a rich diversity of leaf color mutation types in plants [11][12][13][14]. 37Leaf color mutants were divided into five types according to the color classification albino, 38 yellowing, light-green, stripe, spot, etc [15, 16]. Falbel [12] divided the chlorophyll mutant 39 into two categories: (1) mutants that lack chlorophyll b, such as arabidopsis mutants [17], and 40(2) mutants with reduced synthesis of total chlorophyll and chlorophyll b; currently most 41 mutants belong to the latter category. Leaf color characteristics controlled by both nuclear 42 inheritance and cytoplasmic inheritance, may be a quantitative or a quality trait [18]. Most of 43 the leaf color variations are caused by nuclear gene mutation inducing a type of chlorophyll 44 deficiency in higher plants. The leaf color variations are mostly monogenic mutation, while a 45 handful of them are polygenic mutation. Among these mutations, the recessive mutations are 4...

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Characterization and gene mapping of a chlorophyll-deficient mutant clm1 of Triticum monococcum L.

Cited by 30 publications

References 42 publications

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

Examination and genetic analysis of a yellow‐green leaf mutant a269 of sweetpotato

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

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