Characterization and genome analysis of two new Aeromonas hydrophila phages, PZL-Ah1and PZL-Ah8

Yu, Haiyan; Feng, Chao; Raza, Sayed Haidar Abbas; Zhang, Liang; Chi, Teng; Qi, Yanling; Jia, Kaixiang; Zhang, Yang; Wei, Jing; Ai-dong, Qian; Sun, Wuwen; Shan, Xiaofeng; Zhang, Lei

doi:10.1007/s00705-021-05345-y

Cited by 5 publications

(8 citation statements)

References 16 publications

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“…Numerous studies have demonstrated that bacteriophages, particularly members of the Autographiviridae family, have a narrow host range [ 77 , 78 , 79 , 80 ]. On the other hand, some of the phages, including representatives of Autographiviridae , are able to infect multiple strains of the same species of bacteria (e.g., Aeromonas PZL-Ah8) or infect multiple species within the same genus (e.g., Aeromonas PZL-Ah1) or polyvalent ones able to multiply on different bacterial genera [ 81 ]. An exceptional example of polyvalent viruses within Autographiviridae is a group of closely related environmental Atoyac phages infecting bacteria from six genera— Aeromonas , Pseudomonas , Yersinia , Hafnia , Escherichia , and Serratia isolated from the similar sources where the phages originated [ 24 ] ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Bujak

Decewicz

Kitowicz

et al. 2022

Viruses

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In this study, we isolated and characterized three novel virulent Autographiviridae bacteriophages, vB_AspA_Bolek, vB_AspA_Lolek, and vB_AspA_Tola, which infect different Aeromonas strains. These three host–pathogen pairs were derived from the same sampling location—the arsenic-containing microbial mats of the Zloty Stok gold mine. Functional analysis showed they are psychrotolerant (4–25 °C), albeit with a much wider temperature range of propagation for the hosts (≤37 °C). Comparative genomic analyses revealed a high nucleotide and amino acid sequence similarity of vB_AspA_Bolek and vB_AspA_Lolek, with significant differences exclusively in the C-terminal region of their tail fibres, which might explain their host range discrimination. The protein-based phage network, together with a phylogenetic analysis of the marker proteins, allowed us to assign vB_AspA_Bolek and vB_AspA_Lolek to the Beijerinckvirinae and vB_AspA_Tola to the Colwellvirinae subfamilies, but as three novel species, due to their low nucleotide sequence coverage and identity with other known phage genomes. Global comparative analysis showed that the studied phages are also markedly different from most of the 24 Aeromonas autographiviruses known so far. Finally, this study provides in-depth insight into the diversity of the Autographiviridae phages and reveals genomic similarities between selected groups of this family as well as between autographiviruses and their relatives of other Caudoviricetes families.

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Section: Resultsmentioning

confidence: 99%

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Bujak

Decewicz

Kitowicz

et al. 2022

Viruses

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“…The closest hits officially classified at the genus level were to phages from the Chatterjeevirus and Ningirsuvirus phage genera. Bacteriophage JELG-KS1 was, unsurprisingly, most similar (~40% intergenomic similarity) to several of the Aeromonas phages, all of which are still taxonomically unrecognized at the genus level but tentatively represent the same genus within the Studiervirinae subfamily ( Aeromonas phages T7-Ah [ 82 ], PZL-Ah152 [ 83 ], vB_AhaP_PT2 (not described in a dedicated article to the best of our knowledge), and PZL-Ah8 [ 84 ]).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of a Novel Aeromonas salmonicida-Infecting Studiervirinae Bacteriophage, JELG-KS1

Svanberga,

Avsejenko,

Jansons

et al. 2024

Microorganisms

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Representatives of the bacterial genus Aeromonas are some of the most notorious aquaculture pathogens associated with a range of diseases in different fish species. As the world forges toward the post-antibiotic era, alternative options for combating bacterial pathogens are needed. One such alternative option is phage biocontrol. In this study, a novel podophage—JELG-KS1—infecting Aeromonas salmonicida was retrieved from wastewater along with its host strain. The genome of the JELG-KS1 phage is a 40,505 bp dsDNA molecule with a GC% of 53.42% and 185 bp direct terminal repeats and encodes 53 predicted proteins. Genomic analysis indicates that JELG-KS1 might represent a novel genus within the subfamily Studiervirinae. Podophage JELG-KS1 is a strictly lytic phage without any identifiable virulence or AMR genes that quickly adsorbs onto the surface of host cells to initiate a 48 min long infectious cycle, resulting in the release of 71 ± 12 JELG-KS1 progeny virions per infected cell. JELG-KS1 effectively lyses its host population in vitro, even at very low multiplicities of infection. However, when challenged against a panel of Aeromonas spp. strains associated with diseases in aquaculture, JELG-KS1 shows host-specificity that is confined only to its isolation strain, immediately compromising its potential for Aeromonas spp. biocontrol in aquaculture.

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“…Ultracentrifugation was performed to purify HJ05 for the purpose of genome extraction according to a previous report [22]. The genomic DNA of the propagated phages was extracted using the TIANamp Virus DNA/RNA Kit (TIANGEN, Beijing, China) following the manufacturer's instructions.…”

Section: Genome Analysismentioning

confidence: 99%

“…Phagebased biocontrol strategies have been regarded as a promising and environmentally friendly alternative to antibiotics for the treatment of A. hydrophila infection. Recently, various phages have been identi ed and characterized to test their potential against A. hydrophila infection, which at least include Autographiviridae phage Ahy-yong1 [12], Podoviridae phage vB_ AhaP_PZL-Ah8 [13], Caudovirales phage AhMtk13a [14], Podoviridae phage ZPAH7 [15], Caudovirales phage AHP-1[16], Podoviridae phage MJG [17], Caudovirales phage CC2[18], Caudovirales phage PVN02 [19], Caudovirales phage Ahp2 [20], Podoviridae phage PZL-Ah152 [21], Podoviridae phages PZL-Ah1 and PZL-Ah8 [22], Caudovirales phage phiA8-29 [23], Caudovirales phage AhyVDH1 [24], Caudovirales phage vB-AhyM-AP1 [25], Caudovirales phages N21, W3 and G65[26], Podoviridae phages Y71 and Y81[26], Chaseviridae phage pAh6.2TG [27], Siphoviridae phage Akh-2[28], Caudovirales phage VTCCBPA6 [29], Caudovirales phages CF8, PS1, and PS2 [30]. Siphoviridae phages AhSzq-1 and AhSzw-1 [31].…”

Section: Introductionmentioning

confidence: 99%

“…Indeed in vitro and in vivo studies have shown the importance of using phage cocktails rather than single phage preparations to achieve better therapy outcomes [33]. Primary study indicated that combination of A. hydrophila phage vB_ AhaP_PZL-Ah8 and another phage vB_ AhaP_PZL-Ah1 could improve the therapeutic e cacy both in vitro and in vivo [22].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of the lytic Aeromonas phage HJ05,a new member of Caudovirales

Huang

Liu

et al. 2023

Preprint

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Phages are proposed for treating and preventing bacteria diseases largely due to their narrow spectrum of activity and capacity to multiply at the site of infection. Aeromonas hydrophila, a gram-negative and facultatively anaerobic bacterium, is widely distributed in aquatic environments causing diseases opportunistically in almost all freshwater fish displaying various symptoms including Aeromonas septicemia and ulcerative infections. In the present study, a virulent phage of A. hydrophila, designated as HJ05, from natural water was isolated and characterized. The morphological structure of HJ05 was observed by transmission electron microscopy, which indicated that the head of phage HJ05 was icosahedral with a diameter of about 77 nm, and the length of the straight and shrinkable tail could reach 193 nm. Analysis of the biological characteristics of HJ05 revealed the optimal multiplicity of infection (MOI) of 0.1, the latent period of phage was 10 min, the burst period was 40 min, and the burst size was 120 PFU/cell. The titer decreases rapidly from 45–50 ℃ indicating HJ05’s sensitivity to temperature, and optimum pH range for HJ05 replication was widely distributed from pH 5 to 11. Genome analysis of HJ05 revealed a linear doubled-stranded DNA genome of 79124 bp with a G + C content of 58.63%. Comparative genomic analysis revealed that the HJ05 genome has little similarity to any of the currently sequenced Aeromonas phage genomes, indicating that HJ05 does not belong to any of the known genera. Among 94 putative open reading frames identified in the genome, only 45 ORFs encoded proteins displaying significant homology to the known functional proteins in NCBI database. Phylogenetic relatedness analysis of two ORFs (ORF30 encoding head morphogenetic protein and ORF74 encoding DNA polymerase) did indicate HJ05 formed a sister clade with Rhizobium phages. According to the classification criteria of the International Committee on Taxonomy of Viruses, HJ05 was identified to order Caudovirales. This study suggested that HJ05 might represent a novel member of the family Caudovirales, and the lytic activity of HJ05 against A. hydrophila predicted its potential role in phage therapy toward the control of bacterial infections in aquaculture.

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Characterization and genome analysis of two new Aeromonas hydrophila phages, PZL-Ah1and PZL-Ah8

Cited by 5 publications

References 16 publications

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Isolation and Characterization of a Novel Aeromonas salmonicida-Infecting Studiervirinae Bacteriophage, JELG-KS1

Isolation and characterization of the lytic Aeromonas phage HJ05,a new member of Caudovirales

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