Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

Abstract

Background: With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective decontamination method is to utilize diesel-degrading microbes as a means for bioremediation. Here, we present a newly isolated and identified strain of Acinetobacter calcoaceticus ('CA16') as a candidate for the bioremediation of diesel-contaminated areas. Results: Acinetobacter calcoaceticus CA16 was able to survive and grow in minimal medium with diesel as the only source of carbon. We determined through metabolomics that A. calcoaceticus CA16 appears to be efficient at diesel degradation. Specifically, CA16 is able to degrade 82 to 92% of aliphatic alkane hydrocarbons (C n H n + 2 ; where n = 12-18) in 28 days. Several diesel-degrading genes (such as alkM and xcpR) that are present in other microbes were also found to be activated in CA16. Conclusions: The results presented here suggest that Acinetobacter strain CA16 has good potential in the bioremediation of diesel-polluted environments.

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

“…Hence both bacteria isolates were identified as potential hydrocarbon degraders that can utilize components in diesel oil as its carbon source. The present results are agreed with the finding of Ho, Li, McDowell, MacDonald and Yuan (2020), who observed the growth of bacteria on the minimal medium supplemented with 0.2% diesel as the only carbon source to assess their diesel-degrading properties. Carbon source is essential for building cells and important in various metabolic processes for bacteria to grow and multiply.…”

Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

“…A. baumannii is abundant in nature, and can be acquired from water, soil and living organisms. Some strains of Acinetobacter are known to be associate in biodegradation of different number of pollutants such as Permethrin (Zhan et al 2018 ), phenanthrene, 3-chloroaniline (Duc 2016 ), diesel (Ho et al 2020 ), indole (Sadauskas et al 2017 ), malathion (Xie et al 2009 ), deltamethrin (Tang et al 2020 ), azo dye (Sreedharan et al 2021 ) and phenol (Liu et al 2020 ). Biodegradation of cellulose, chitin, pectin, fluoranthene, and phenanthrene by Actinetobacter from deep Sea Sediments were reported by (Chen et al 2016 ).…”

Acinetobacter tandoii ZM06 Assists Glutamicibacter nicotianae ZM05 in Resisting Cadmium Pressure to Preserve Dipropyl Phthalate Biodegradation

“…The Cd(II) concentration in the supernatant was investigated by ICP-MS (Agilent Technologies 7800 ICP-MS) analysis [ 28 ]. Quantitative PCR (qPCR) was used to quantify the ratio of DNA levels of the specific estG / xcpR genes to determine the ratios of ZM05 to ZM03 in the coculture system under Cd(II) stress [ 29 , 30 ]. The primers used in qPCR are listed in Supplementary Table S1 .…”