Characterization and haplotype study of 6 novel STR markers related to the KCNQ1 gene in heterogeneous cardiovascular disorders in the Iranian population

Abstract

Background/aim: The KCNQ1 gene has a significant role in long QT syndrome, Jervell and Lange-Nielsen syndrome, familial atrial fibrillation, and short QT syndrome. Analyzing such heterogeneous disorders, six novel short tandem repeat (STR) markers around the KCNQ1 gene were found and evaluated in a healthy population, and other statistical traits of the markers were detected. Materials and methods: Using Tandem Repeats Finder (TRF) and Sequence-Based Estimation of Repeat Variability (SERV) software, STR markers were detected with valid tetra-and pentanucleotide repeats. The markers were investigated for a total of 60 unrelated Iranian healthy individuals and analyzed using GenAlEx 6.502 and Cervus 3.0.7. Results: A total of 77 haplotypes was detected, of which 25 haplotypes were unique and the others occurred at least two times. The number of haplotypes per locus ranged from 7 to 18 with the highest frequency of 69.2%, and the observed heterozygosity was calculated as 0.589. The power of discrimination ranged from 0.70 to 0.96. Five of the markers meet Hardy-Weinberg equilibrium. Conclusion: A novel panel of STR markers was described with high allele heterozygosity and segregation in every locus, which may lead to faster and more credible recognition of the disease-inducing KCNQ1 gene and allow it to be used for human identity testing and prenatal diagnosis.

The association of social rank with paternity efficiency in competitive mating flocks of Zi goose ganders (Anser cygnoides L.)

“…The Gene Marker V1.91 Software (Beijing Huasheng Hengye Technology Co. Ltd., Beijing, China) was utilized to determine the microsatellite marker genotype of each sample. DNA sample genotype results were determined by Servus 3.0.7 ( Amirian et al., 2019 ). The number of alleles per locus varied from 5 to 13 with a mean value of 7.05.…”

Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China

“…Amplification products were separated on 2.5% agarose gels in 0.5× TAE buffer, using a 50-bp DNA ladder as a DNA molecular marker. Finally, the polymorphism of primers was assessed using CERVUS 3.0 [ 37 ], and the primers in which PIC exceeded 0.5 were retained for use in genetic diversity analyses [ 38 ].…”

The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

“…Developing and validating STR markers was an essential step toward their use in genotyping. The validated STR markers were used to estimate their frequency in various populations worldwide [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39].…”