Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates

Mudgil, Priti; Kamal, Hina; Gan, Chee‐Yuen; Maqsood, Sajid

doi:10.1016/j.foodchem.2018.03.082

Cited by 184 publications

(131 citation statements)

References 39 publications

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“…This unique characteristic of camel milk insulin gives it an advantage over other mammalian insulin and could support its therapeutic value in people with diabetes when given orally. Second, a group of researchers from the United Arab of Emirates and Ireland recently identified novel peptides in camel milk hydrolysates that inhibit dipeptidyl peptidase-4 (Mudgil et al, 2018;Nongonierma et al, 2018Nongonierma et al, , 2019. This enzyme plays a major role in glucose metabolism Korish et al: ANTITHROMBOTIC ACTIONS OF BOVINE AND CAMEL MILK Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the hypoglycemic and antithrombotic (anticoagulant) actions of whole bovine and camel milk in streptozotocin-induced diabetes mellitus in rats

Korish

Gader

Alhaider

2020

Journal of Dairy Science

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People with diabetes are at higher risk of fatal thromboembolic accidents in the cerebral and coronary circulations, especially stroke and ischemic heart disease. We have previously described the hypoglycemic, hypolipidemic, and anticoagulant activity of orally administered camel milk in streptozotocin-induced diabetic rats. In the present study in the same animal model, we extended these observations by comparing camel milk and the more available and widely consumed bovine milk with respect to their antidiabetic and antithrombotic actions. Rats were rendered diabetic by intraperitoneal streptozotocin (65 mg/kg), and then camel milk or bovine milk was administered orally for 8 wk. We evaluated the changes in body weight, fasting blood glucose, glucose tolerance, blood coagulation profile, and platelet function. Diabetic rats developed weight loss, hyperglycemia, glucose intolerance, inhibition of platelet aggregation responses to arachidonic acid and adenosine diphosphate, a marked decrease (>50%) in plasma fibrinogen levels, and short activated partial thromboplastin time. Treatment with camel milk or bovine milk reversed these abnormalities, resulting in weight gain, decreased blood glucose levels, and improved glucose tolerance. Despite the more remarkable antidiabetic action of camel milk, treatment with bovine milk was more effective in correcting plasma fibrinogen levels and restoring inhibited platelet aggregation responses. Long-term administration of camel milk or bovine milk counteracted streptozotocin-induced metabolic manifestations in rats, maintained platelet function, and abolished coagulopathy-associated fibrinogen consumption. Notably, the antidiabetic effect of camel milk was more pronounced than that of bovine milk, but bovine milk exhibited more potent anticoagulant activity than camel milk. These findings should encourage further clinical trials to assess the efficiency of camel milk and bovine milk or their derived peptides as food supplements or potential nonpharmacological therapies for dysglycemia and the vascular complications of diabetes mellitus.

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Section: Discussionmentioning

confidence: 99%

Comparison of the hypoglycemic and antithrombotic (anticoagulant) actions of whole bovine and camel milk in streptozotocin-induced diabetes mellitus in rats

Korish

Gader

Alhaider

2020

Journal of Dairy Science

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“…Peptides also possess antioxidative properties (Jara et al, 2018;Ma et al, 2018), whereas some sequences act as anti-cancer agents by inhibiting excessive cell proliferation (Chi et al, 2015;Vasquez-Villanueva et al, 2018). As antidiabesity agents, peptide inhibition of lipase (Ngoh et al, 2017), dipeptidyl peptidase-IV (Nongonierma et al, 2017;Mudgil et al, 2018) and carbohydrases (a-amylase and a-glucosidase) have also been reported (Ngoh & Gan, 2016;Uraipong & Zhao, 2016a;. However, there is scant information on the ability of food protein hydrolysates to possess multifunctional properties by inhibiting activities of a-amylase, a-glucosidase and lipase.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the in vitro activities of α‐amylase, α‐glucosidase and pancreatic lipase by yellow field pea (Pisum sativum L.) protein hydrolysates

Awosika

Aluko

2019

Int J of Food Sci Tech

111

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Summary The aim of this work was to produce yellow field pea protein‐derived peptides as inhibitors of α‐amylase, α‐glucosidase and pancreatic lipase activities. A pea protein concentrate was hydrolysed with alcalase, chymotrypsin, pepsin or trypsin and the hydrolysates separated into different fractions (<1, 1–3, 3–5, 5–10 kDa) by membrane ultrafiltration. Peptide sequence analysis showed that the alcalase hydrolysate had higher levels of di‐ and tripeptides when compared with the chymotrypsin, pepsin and trypsin hydrolysates. The peptide fractions inhibited α‐amylase and α‐glucosidase activities at levels that were similar to the unfractionated hydrolysates. The peptides were more active against α‐amylase (inhibition at μg level) than α‐glucosidase (mg level). In contrast, the fractionated peptides had reduced ability (IC50 >4.2 mg mL−1) when compared with the unfractionated hydrolysate (IC50 <4.2 mg mL−1) to inhibit lipase activity. Enzyme kinetic studies revealed that the peptides reduced α‐amylase activity through competitive inhibition. However, inhibition of α‐glucosidase activity was non‐competitive.

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“…Hyperlipidemia is the best-known disease in connection with a sedentary lifestyle, inherited genetic disorder, high-fat diets, diabetes, and obesity [3]. In recent years, the population of hyperlipidemia has been increasing gradually around the world due to the unhealthy lifestyles, such as smoking, high fat acid (FA) and sugar diets, and physical inactivity [4]. Therefore, synthetic drugs including bile acid binding resins, nicotinic acid derivatives, and cholesterol absorption inhibitors were applied to regulate the lipid levels of plasma [5]; however, their potential toxicities cause great trouble to the patient and limit their long-term applications [6].…”

Section: Introductionmentioning

confidence: 99%

“…To date, active ingredients from natural resources including polysaccharides, quinones, alkaloids, flavonoids, steroidal saponins, and proteins and peptides, have been identified as potential hypolipidemic agents because they could accelerate the catabolism of exogenous lipid, suppressing the biosynthesis of endogenous lipids and inhibiting lipid absorption and peroxidation [4,[8][9][10]. Among them, food-derived peptides attract extensive attention because they have physiological functions beyond their high nutritional values and can act as drugs and functional products [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates

Cited by 184 publications

References 39 publications

Comparison of the hypoglycemic and antithrombotic (anticoagulant) actions of whole bovine and camel milk in streptozotocin-induced diabetes mellitus in rats

Comparison of the hypoglycemic and antithrombotic (anticoagulant) actions of whole bovine and camel milk in streptozotocin-induced diabetes mellitus in rats

Inhibition of the in vitro activities of α‐amylase, α‐glucosidase and pancreatic lipase by yellow field pea (Pisum sativum L.) protein hydrolysates

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

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