Characterization and immobilization of protease secreted by the fungus Moorella speciosa

Abstract

Protease was extracellularly produced in submerged fermentation by the fungus Moorella speciosa with maximum activity of 8.6 × 10 3 U/mL. The optimal pH and temperature for enzyme activity were 6.78 and 60.88 °C, respectively. The enzyme was incubated in the presence of several ions at concentrations of 0.1 M and 0.01 M to address the effect on enzyme activity. Enzyme activity was increased by 56% and 130% in the presence of 0.1 M BaCl 2 and of 0.01 M Na 2 SO 4 , respectively. The V max and K m values were 0.01474 U/min/mg protein and 0.04190 mg/mL, respectively. The enzyme retained about 90% of enzymatic activity at 90 °C. Among the methods tested for enzyme immobilization, adsorption onto MAT540 carrier led to the most promising results, since after 15 reuse cycles up to 60% of the initial catalytic activity was retained. Entrapment in calcium alginate matrix allowed to retain up to 51% of the initial catalytic activity after 8 reuse cycles. This protease from M. speciosa, in either free or immobilized form, can be foreseen as a useful biocatalytic tool in process design by reducing operating costs, decreasing the use of chemical processing and, consequently, meeting the global demand for clean technologies.

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

“…Many industries namely textiles, medicines, leather, food and detergents have extensively accommodated the use of enzymes in their practices since the emergence of enzymology [1]. It is estimated that by 2023, the global industrial enzyme market will escalate rapidly by 4.9 % to reach US$7 billion [1].…”

“…Many industries namely textiles, medicines, leather, food and detergents have extensively accommodated the use of enzymes in their practices since the emergence of enzymology [1]. It is estimated that by 2023, the global industrial enzyme market will escalate rapidly by 4.9 % to reach US$7 billion [1]. With the transformation of the chemical processes to enzymatic processes for goods and commodities assembly, the gradual adaptation of industrial-scale production processes over the years may contribute largely to this growth [2].…”

“…With the transformation of the chemical processes to enzymatic processes for goods and commodities assembly, the gradual adaptation of industrial-scale production processes over the years may contribute largely to this growth [2]. The broad biochemical diversity of microorganisms and the achievability of large-scale production are the main factors that turn microorganisms into the most commonly used source for the production of industrial enzymes [1]. In addition, enzymes have been increasingly used in several industries and therefore, it is a pressing task to find new microbial isolates that can produce functional enzymes to meet this growing demand [3].…”