Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces

Zuo, Fanglei; Yu, Rui; Feng, Xiujuan; Chen, Lili; Zeng, Zhu; Khaskheli, Gul Bahar; Ma, Huiqin; Chen, Shangwu

doi:10.1007/s13213-015-1187-x

Cited by 83 publications

(68 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Organic acids have a significant inhibitory effect on gram-negative bacteria [55]. Healthy oral microbiota is usually based on ability of probiotics to inhibit the development of oral cavity-related pathogens [56].this study is in support of [57].…”

Section: Anti-microbial Activity Of Potential Probiotic Lactic Acid Bsupporting

confidence: 51%

Anti-microbial activity of potential probiotic lactic acid bacteria against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Nebyou

Sharma

Taneja

2020

Preprint

View full text Add to dashboard Cite

Acid and bile tolerance activity of lactic acid bacteria isolated from bovine milk. AbstractLactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora, and dairy products able to cope up and exist in diverse environmental ranges. Samples were collected using sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization. Gram test and catalase activity were examined after microscopically distinct colonies were sub-cultured to pure colonies based on standard gram and catalase test protocols. Subsequently, these bacteria were characterized for their ability to grow at various salt concentrations (5%,10%, and15%) and temperature gradients (15°C, 30°C, 45°C). Acid-tolerance was analyzed by growing the colonies in MRS broth adjusted to acidic pH (pH 3) and pH 7.2 (control). Bile tolerance of LAB isolates was assayed by growing in 0.3% bile-supplemented MRS agar. Bile salt hydrolase (BSH) activity was studied by growing 10 μ l of the prepared overnight culture on BSH screening media containing MRS agar plate supplemented with bile salts. The LAB isolates were checked for antimicrobial activity by agar well diffusion assay. All isolates found gram-positive, catalase-negative and non-motile, convex elevation and entire margin. All LAB isolates were able to grow at 5-10% Nacl concentration, whereas moderately grow at 10% concentration but rarely grow at 15% Nacl concentration. BCM2, BBM3 and BGM1 record the highest acidic resistance viability percentage 94.9%, 92.7%, and 91.8% respectively. BCM3 has the lowest acidic resistance with a viable percentage of 87.4%.2 BBM1 records the highest bile tolerance activity whereas BCM2 has the lowest bile tolerance. All isolates were found BSH positive. The study reveals LAB isolates showed a putative probiotic potential. Article highlightsLAB are the main ingredients of probiotic products commercially available in the market nowadays. To effectively functioning the host gastrointestinal tract probiotics, need to have certain criteria like acid and bile tolerance this study reveals • Acid tolerance activity of LAB isolated from bovine milk • Bile tolerance activity of LAB isolated from bovine milk • Bile salt hydrolase (BSH) activity of LAB isolates • Antimicrobial activity of LAB against MRSA

show abstract

Section: Anti-microbial Activity Of Potential Probiotic Lactic Acid Bsupporting

confidence: 51%

Anti-microbial activity of potential probiotic lactic acid bacteria against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Nebyou

Sharma

Taneja

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…To determine the hydrophobicity of isolated strains, the MATH method by Vinderola et al [22] was used, and to determine the auto-aggregation, the method of Zuo et al [7] was used. In both cases, bacterial suspensions grown overnight in an MRS broth were harvested by centrifugation at 6000 rpm for 8 min at laboratory temperature.…”

Section: Evaluation Of Hydrophobicity and Auto-aggregationmentioning

confidence: 99%

“…They have a long history of use and have the generally recognized as safe (GRAS) status. The members of the Bifidobacterium genus comprise a dominant group of human adult intestinal microbiota and represent up to 90% of the fecal anaerobic strains of the breast-fed infants [7,8]. The genus Lactobacillus belongs to a group of lactic acid bacteria (LAB) and includes a variety of species, such as Lactobacillus plantarum, L. fermentum, L. rhamnosus, L. acidophilus, L. reuteri, L. delbrueckii subsp.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Krausová¹,

Hyršlová²,

Hynstova³

2019

Fermentation

155

View full text Add to dashboard Cite

We isolated several strains of human and animal origin, focusing on the genera Lactobacillus and Bifidobacterium. Samples of cow colostrum, feces and saliva of calves and piglets, and infant feces were acquired, bacterial strains were isolated and identified, and twenty strains of lactobacilli and bifidobacteria were shortlisted for further in vitro analysis of adhesion capacity to human cells, surface hydrophobicity, and auto-aggregation. Adhesion capacity was evaluated using a mixture of Caco-2 and HT-29-MTX cells and hydrophobicity was measured using the microbial adhesion to the hydrocarbon method. Lactobacillus reuteri was the most frequently isolated species, followed by L. casei subsp. paracasei and L. gasseri; all strains were isolated from infant feces. Bifidobacteria were represented by the species B. longum (infant feces), and B. thermophilum and B. pseudolongum (calves, piglets). All twenty strains showed good adhesion capacities to the mixed cell culture (17.7–37.2%), particularly, L. reuteri isolates K7 and K14 (37.2% and 35.5%, respectively). In hydrophobicity and auto-aggregation assays, strain-specific differences irrespective of the origin or taxonomic group were observed. Hydrophobicity values varied considerably (from 6.1% to 87.4%), whereas auto-aggregation ability ranged from 21.7% to 69.7%. No relation was observed between hydrophobicity and adhesion capacity; instead, auto-aggregation was apparently related with adhesion.

show abstract

“…First, they showed higher survival rates in simulated gastric juice with 90, 70, 91 and 88% respectively (Zuo et al . ). Second, strains IF3‐211 and IF1‐04 showed good survival rates in simulated bile juices (54 and 55%), higher than the two reference strains (46 and 41%), while strains IF1‐11 and IF3‐111 were slightly sensitive to bile juices (less than 50% survival rate) (our unpublished data).…”

Section: Resultsmentioning

confidence: 97%

Bifidobacteria possess inhibitory activity against dipeptidyl peptidase-IV

Zeng

Luo

Zuo

et al. 2016

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces

Cited by 83 publications

References 77 publications

Anti-microbial activity of potential probiotic lactic acid bacteria against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Anti-microbial activity of potential probiotic lactic acid bacteria against Methicillin-Resistant Staphylococcus Aureus (MRSA)

In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Bifidobacteria possess inhibitory activity against dipeptidyl peptidase-IV

Contact Info

Product

Resources

About