Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation

Teillet, Jeffrey; Martinez, Quentin; Tijunelyte, Inga; Chami, Bayan; Bancaud, Aurélien

doi:10.1039/d0sm00475h

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…Future developments for the μLAF technology include the enhancement of the collection yield to more than 50%, keeping the collection volume to 50 μL. This task requires the minimization of the dispersion during the collection step because the convection to the outlet is associated to Taylor dispersion . Reducing Taylor dispersion can be achieved by shortening the lag time of ∼100 s between the drop in the electric field and the collection of the outlet (see, e.g., Figure A).…”

Section: Discussionmentioning

confidence: 99%

“…This task requires the minimization of the dispersion during the collection step because the convection to the outlet is associated to Taylor dispersion. 26 Reducing Taylor dispersion can be achieved by shortening the lag time of ∼100 s between the drop in the electric field and the collection of the outlet (see, e.g., Figure 2A). Enhancing the performance of μLAF in different MW ranges also constitutes an important milestone.…”

Section: ■ Discussionmentioning

confidence: 99%

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Size Fractionation of Milliliter DNA Samples in Minutes Controlled by an Electric Field of ∼10 V

Bruand,

Tijunelyte,

Castinel

et al. 2023

Anal. Chem.

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DNA size fractionation is an essential tool in molecular biology, which is used to isolate targets in a mixture characterized by a broad molecular weight (MW) distribution. Microfluidics was thought to provide opportunity to create devices capable of enhancing and speeding up the classical fractionation processes. However, this conjecture met limited success due to the low mass and/or volume throughput of these technologies. We describe the µLAF (µLaboratory for DNA Fractionation) technology for DNA size selection based on the stacking of molecules on films of ~100 µm in thickness with 10 5 pores of ~2 µm in diameter. Size selection is achieved by controlling the regime of electrohydrodynamic migration in the pores through the temporal modulation of an electric field. This technology allows the processing of milliliter-scale samples containing a DNA mass of several hundreds of ng within ~10 minutes, and the selection of DNA in virtually any size window spanning 200 to 1000 bp. We demonstrate that one operation suffices to fractionate sheared genomic DNA in up to six fractions with collection efficiencies of ~20-40 % and enrichment factors of ~1.5-3 fold. These performances compare favorably in terms of speed and versatility to those of current standards.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%