Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant Acinetobacter spp

Carvalho, Andreia; Cardoso, Luís Leónidas; Nogueira, Hadison Santos; Menezes, Elytânia Veiga; Xavier, Mauro Aparecido de Sousa; Barreto, Nair Amélia Prates; Fernandes, L.F.; Xavier, A.R.E.O.

doi:10.4238/gmr.15038608

Cited by 5 publications

(5 citation statements)

References 21 publications

(40 reference statements)

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“…Three sectors of the hospital, the ICU, wards, and apartments, with very distinctive characteristics in terms of both the types of patients and the physical structures, were the probable sites of acquisition/transmission of the bacterium. Similar results, including the genetic profiles of the patients, the place and time when they were hospitalized, were observed in a previous study of nosocomial A. baumannii isolates from the same hospital (Carvalho et al, 2016;Cheikh et al, 2018).…”

Section: Discussionsupporting

confidence: 88%

Research Article Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant <i>Klebsiella pneumoniae</i>

Lima¹,

Carvalho²,

Xavier³

et al. 2019

Genet. Mol. Res.

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We characterized six drug-resistant nosocomial isolates of Klebsiella pneumoniae obtained in a hospital located in northern Minas Gerais State, Brazil, by determining their antibiotic sensitivity profiles, detecting the bla KPC genetic marker and examining their clonal relationships. All isolates were found to be extensively drug resistant. A PCR assay was used to confirm the identity of the isolates as K. pneumoniae and assess the bla KPC gene. All isolates tested positive for the bla KPC gene, which is related to carbapenem resistance. The genetic profiles and clonal relationships among the isolates were evaluated by ERIC-PCR. All the isolates were in a single group with two distinct subgroups. Analysis of the ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (1): gmr18172 E.R. Lima et al. 2 genetic diversity among the isolates revealed that five of the six were clones, which suggests cross-transmission in this hospital environment. Five of the patients died from infection. We describe the first detection of KPC-producing K. pneumoniae isolates from a hospital in northern Minas Gerais state.

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Section: Discussionsupporting

confidence: 88%

Research Article Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant <i>Klebsiella pneumoniae</i>

Lima¹,

Carvalho²,

Xavier³

et al. 2019

Genet. Mol. Res.

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“…The bands sharing analysis among these isolates revealed the presence or absence of 1 or 3 bands in disagreement, which leads the authors to hypothesize possible mutation among the isolates. Carvalho et al (2016) analyzing the genetic profile of nosocomial isolates of Acinetobacter baumannni through DNA polymorphism analysis by ERIC-PCR also suggested the same hypothesis for isolates with high genetic similarity.…”

Section: Resultsmentioning

confidence: 75%

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Xavier¹,

Almeida²,

Souza³

et al. 2017

Genet. Mol. Res.

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The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of antimicrobial susceptibility, the presence of femA gene, the genetic relationships among isolates of S. aureus obtained from milk originating from flocks diagnosed with subclinical mastitis in nine rural properties in the northern of Minas Gerais State. To this end, 498 samples of bovine milk tested positive for the California mastitis test (CMT) were subjected to morphological methods and biochemical patterns for microbiological presumptive identification of S. aureus. The PCR test with the genetic marker femA was used to confirm the species S. aureus. All the 26 isolates presumptively identified as S. aureus amplified a fragment of 132 bp corresponding to the femA gene. The profile of antimicrobial susceptibility was performed according to the disk-diffusion methodology and two isolates were susceptible to all the antibiotics tested. The drug multiresistence was found in 80.76% of the isolates. The determination of the genetic profile and the clonal relationship among the isolates was performed by the method of DNA RAPD-PCR polymorphism. The S. aureus isolates were divided into two groups with 26 distinct subgroups. The analysis of RAPD-PCR showed no genetic diversity among them, heterogeneous profile and absence of clonality.

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“…The presence of genes that encode the expression of carbapenem-type beta-lactamases has been described in Acinetobacter spp. [19,20] and Klebsiella pneumoniae [21,22]. These organisms are also potential etiologic agents of bovine mastitis [23][24][25][26], as well as disseminators of resistance genes to other pathogens [27].…”

Section: Introductionmentioning

confidence: 99%

“…These organisms are also potential etiologic agents of bovine mastitis [23][24][25][26], as well as disseminators of resistance genes to other pathogens [27]. In Acinetobacter baumannii, resistance to carbapenem agents can be attributed to the presence of the gene bla Oxa23 [19,20] and to the gene bla KPC in K. pneumoniae [21,22]. The presence of the gene bla Oxa23 has already been reported in pathogens other than A. baumannii, confirming the transferability of this gene to other species of microorganisms [28,29].…”

Section: Introductionmentioning

confidence: 99%

Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis

et al. 2019

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Background and Aim: The term ESKAPE, recognized by the WHO, is an acronym, which refers to the pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp., which is extremely virulent and multidrug-resistant. Although the term is used to designate nosocomial pathogens, in a milking environment, strains of Methicillin-resistant S. aureus have been isolated from cattle diagnosed with clinical and subclinical mastitis. Resistant strains may be involved in the transfer of genes conferring resistance to beta-lactam antimicrobials among the species of microorganisms related to mastitis etiology. This study aimed to trace the phenotypic and genotypic profiles of susceptibility to beta-lactams in S. aureus isolated from milk of cattle diagnosed with subclinical mastitis obtained from different rural properties located in the North of Minas Gerais State, Brazil. Materials and Methods: Sixteen microorganisms previously identified as S. aureus isolated from milk of cattle diagnosed with subclinical mastitis were submitted to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), mass spectrometry, and polymerase chain reaction (PCR) analysis for microbial species confirmation. The S. aureus beta-lactams antimicrobial phenotypic resistance profile was investigated by disk diffusion method. PCR methods were also performed to investigate the S. aureus genotypic beta-lactams resistance profile. For this purpose, blaZ, mecA, mecALGA251, blaOxa23, and blaKPC genes were screened among S. aureus isolates. The genetic diversity of S. aureus by fingerprint random amplified polymorphic DNA (RAPD)-PCR was also performed in this study. Results: All isolates showed phenotypic resistance to at least three beta-lactams, among which was meropenem. None of the isolates tested positive for the genes mecALGA251, blaOxa23, and blaKPC; however, the presence of the genes blaZ and mecA was detected among the isolates. The fingerprint analysis divided isolates into two distinct groups and 15 different subgroups. Despite the presence of clonality among the isolates, the PCR-RAPD analysis unveiled a heterogeneous profile with genetic diversity among the S. aureus isolates. Conclusion: In this study, we identified beta-lactams resistant S. aureus strains isolated from the milk of cows diagnosed with subclinical mastitis. The S. aureus beta-lactams resistance was investigated using a phenotypic and genotypic approach. We believe that molecular epidemiology, improved knowledge, and genetic basis of resistance to beta-lactams might assist in asserting guidelines for better management practices of dealing with subclinical mastitis and mapping of origin of resistant pathogens in the studied Brazilian area.

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Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant Acinetobacter spp

Cited by 5 publications

References 21 publications

Research Article Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant <i>Klebsiella pneumoniae</i>

Research Article Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant <i>Klebsiella pneumoniae</i>

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis

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