Characterization and morphology analysis of degradable poly(l-lactide) film in in-vitro gastric juice incubation

Chang, Hao‐Ming; Huang, Chun-Chiang; Tsai, Hsieh‐Chih; Imae, Toyoko; Hong, Po‐Da

doi:10.1016/j.apsusc.2012.02.087

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…The same trend was observed by Keridou et al while degrading P4HB films [ 8 ]. Also, this result is consistent with the degradation of other biopolyesters, for instance PLA [ 33 ].…”

Section: Resultssupporting

confidence: 89%

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

et al. 2022

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A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate—P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.

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“…The same trend was observed by Keridou et al while degrading P4HB films [ 8 ]. Also, this result is consistent with the degradation of other biopolyesters, for instance PLA [ 33 ].…”

Section: Resultssupporting

confidence: 89%

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

et al. 2022

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“…PLLA has been widely studied for bone regeneration due to its degradability, biocompatibility, and process ability [ 19 - 21 ]. However, there are several aspects of PLLA that remain problematic with respect to its application in biomedical fields, including hydrophobicity, lack of bioactivity, and release of acidic degradation by-products that may cause inflammation when implanted in vivo [ 22 , 23 ]. In the present study, in vitro and in vivo studies were used to determine whether PLLA mesh, when used as a subcutaneous implantable scaffold for plastic surgery, undergoes mechanical and biocompatible changes.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, there were no changes in the percentage of collagen fibers in subcutaneous tissue around the PLLA mesh during experiments. Conversely, it has been reported that PLLA may induce an inflammatory reaction due its hydrophobicity, low bioactivity, and release of acidic degradation by-products in musculoskeletal tissue and in vitro when incubated with gastric juice [ 22 , 23 ]. Taken together with the results of this study, PLLA mesh may not cause inflammation in subcutaneous tissue, but may cause inflammation in other tissues or under specific incubation conditions.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation and Biocompatibility of Poly L-lactic Acid Implantable Mesh

2017

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PurposeThe purpose of this study was to evaluate the biodegradation and biocompatibility of poly L-lactic acid (PLLA) implantable mesh under in vitro and in vivo conditions.MethodsPLLA mesh was examined for changes in weight and tensile strength. The histology of the tissue around the PLLA implant was also evaluated.ResultsThe weight and tensile strength of the PLLA prosthesis was stable for 180 days. In addition, the surface of the PLLA mesh was not digested under in vitro or in vivo conditions as determined by scanning electron microscope. Histologically, there were no significant changes in the diameters of implanted PLLA mesh and subtype fibers over the course of 180 days. Likewise, there were no significant changes in the number of inflammatory and mast cells after 180 days, nor was there an increase in the percentage of collagen surrounding the PLLA mesh.ConclusionsThe results indicate that PLLA prostheses have good rigidity and biocompatibility in vivo and in vitro.

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“…However, there are still some problems that need to be solved for PLLA application in the biomedical field, such as the hydrophobicity, lack of bioactivity and the release of acidic degradation by-products, which might cause inflammation when implanted in vivo [13,14]. It is well known that inorganic bioactive materials, such as hydroxyapatite, tricalcium phosphate, bioglasses and calcium silicate, have been incorporated into degradable polymers to develop inorganic/organic biocomposites [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…for 84 days, and the Tris-HCl solution was refreshed once a week. At specific time intervals (1,3,5,7,14,21,28,35,49,63 and 84 days), the specimens were washed thoroughly with deionized water, then dried at 808C in an oven for 12 h. …”

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confidence: 99%

Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly( l -lactide) composite

Liu

Tang

Qian

et al. 2015

J. R. Soc. Interface.

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Bioactive mesoporous diopside (m-DP) and poly(L-lactide) (PLLA) composite scaffolds with mesoporous/macroporous structure were prepared by the solution-casting and particulate-leaching method. The results demonstrated that the degradability and bioactivity of the mesoporous/macroporous scaffolds were significantly improved by incorporating m-DP into PLLA, and that the improvement was m-DP content-dependent. In addition, the scaffolds containing m-DP showed the ability to neutralize acidic degradation products and prevent the pH from dropping in the solution during the soaking period. Moreover, the scaffolds containing m-DP enhanced attachment, proliferation and alkaline phosphatase activity of MC3T3-E1 cells, which were also m-DP content-dependent. Furthermore, the histological and immunohistochemical analysis results showed that the scaffolds with m-DP significantly promoted new bone formation and improved the materials degraded in vivo, indicating good biocompatibility. The results suggested that the mesoporous/macroporous scaffolds of the m-DP/PLLA composite with osteogenesis had a potential for bone regeneration.

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Characterization and morphology analysis of degradable poly(l-lactide) film in in-vitro gastric juice incubation

Cited by 10 publications

References 34 publications

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

Biodegradation and Biocompatibility of Poly L-lactic Acid Implantable Mesh

Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly( l -lactide) composite

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