Characterization and neural differentiation of mouse embryonic and induced pluripotent stem cells on cadherin-based substrata

Haque, Amranul; Yue, Xiaoshan; Motazedian, Ali; Tagawa, Yoh‐ichi; Akaike, Toshihiro

doi:10.1016/j.biomaterials.2012.04.003

Cited by 51 publications

(64 citation statements)

References 29 publications

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“…Mesoendoderm differentiation was less efficient on the EcadFc-coated substrate (Fig. 3b), which was consistent with previous report that EcadFc-coated substrate promotes ESC pluripotency [22]. Hence, the EcadFc-coated substrate could elicit a bona fida E-cadherin-mediated adhesive response despite the fact that cells did not exhibit a typical epithelial morphology with compact cellecell junctions.…”

Section: Control Over Mesoendoderm Differentiation Patterns By Modulasupporting

confidence: 91%

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Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation

Toh

Xing

2015

Biomaterials

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Section: Control Over Mesoendoderm Differentiation Patterns By Modulasupporting

confidence: 91%

“…3a). EcadFc has been used to mimic E-cadherinmediated adhesion on tissue culture polystyrene substrates in a concentration dependent manner [21,22] (Fig. S5).…”

Section: Control Over Mesoendoderm Differentiation Patterns By Modulamentioning

confidence: 99%

Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation

Toh

Xing

2015

Biomaterials

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“…85 In addition, it has been reported that N-cadherin regulates neuronal differentiation from induced pluripotent stem cells (iPSCs). 98,99 These findings support the notion that the downregulation of the N-cadherin level in nascent neurons and IP is required for the onset of neuronal differentiation of NPCs. …”

Section: Regulation Of Differentiation Of Npcs By N-cadherinsupporting

confidence: 78%

N-cadherin-based adherens junction regulates the maintenance, proliferation, and differentiation of neural progenitor cells during development

Miyamoto

Sakane

Hashimoto

2015

Cell Adhesion & Migration

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This review addresses our current understanding of the regulatory mechanism by which N-cadherin, a classical cadherin, affects neural progenitor cells (NPCs) during development. N-cadherin is responsible for the integrity of adherens junctions (AJs), which develop in the sub-apical region of NPCs in the neural tube and brain cortex. The apical domain, which contains the sub-apical region, is involved in the switching from symmetric proliferative division to asymmetric neurogenic division of NPCs. In addition, Ncadherin-based AJ is deeply involved in the apico-basal polarity of NPCs and the regulation of Wnt-b-catenin, hedgehog (Hh), and Notch signaling. In this review, we discuss the roles of N-cadherin in the maintenance, proliferation, and differentiation of NPCs through components of AJ, b-catenin and aE-catenin.

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“…Neural differentiation of PSCs is associated with the switch from E-cadherin expression to N-cadherin expression. Hence, recombinant ECM components based on E-cadherin and N-cadherin hybrid substratum were also shown to support neural differentiation of ESCs and iPSCs [62] . Chambers et al [12] , 2009…”

Section: Effects Of Ecm On Npc Differentiation From Pscsmentioning

confidence: 99%

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

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Neural cells differentiated from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells (OPCs) and neural progenitor cells (NPCs) have been derived from PSCs recently due to the advancements in understanding the developmental signaling pathways. Extracellular matrices (ECM) have been shown to play important roles in regulating the survival, proliferation, and differentiation of neural cells. To improve the function and maturation of the derived neural cells from PSCs, understanding the effects of ECM over the course of neural differentiation of PSCs is critical. During neural differentiation of PSCs, the cells are sensitive to the properties of natural or synthetic ECMs, including biochemical composition, biomechanical properties, and structural/topographical features. This review summarizes recent advances in neural differentiation of human PSCs into OPCs and NPCs, focusing on the role of ECM in modulating the composition and function of the differentiated cells. Especially, the importance of using three-dimensional ECM scaffolds to simulate the in vivo microenvironment for neural differentiation of PSCs is highlighted. Future perspectives including the immediate applications of PSC-derived neural cells in drug screening and disease modeling are also discussed.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Pluripotent stem cells; Neural differentiation; Extracellular matrix; Three-dimensional; Drug screening Core tip: Neural cells derived from human pluripotent stem cells (hPSCs), including oligodendrocyte progenitor cells and neural progenitor cells, emerge as an unlimited and physiologically relevant cell source for drug screening, disease modeling, and regenerative medicine. Natural and synthetic extracellular matrices play an important role in regulating neural differentiation, cell migration, and the derived neural cell maturation. Recent advances in neural differentiation of hPSCs on extracellular matrices in 2-D and 3-D systems are reviewed in this paper. The immediate applications of the derived neural cells in drug screening and disease modeling are also discussed.

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Characterization and neural differentiation of mouse embryonic and induced pluripotent stem cells on cadherin-based substrata

Cited by 51 publications

References 29 publications

Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation

Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation

N-cadherin-based adherens junction regulates the maintenance, proliferation, and differentiation of neural progenitor cells during development

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

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