Characterization and Optimization of Bioflocculant Exopolysaccharide Production by Cyanobacteria Nostoc sp. BTA97 and Anabaena sp. BTA990 in Culture Conditions

Tiwari, Onkar Nath; Khangembam, Romi; Shamjetshabam, Minerva; Sharma, Aribam Subhalaxmi; Oinam, Gunapati; Brand, Jerry J.

doi:10.1007/s12010-015-1691-2

Cited by 52 publications

(27 citation statements)

References 64 publications

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“…Sufficient content of uronic acid in a bioflocculant molecule can provide carboxyl groups to the molecular chain, which are preferred for the adsorption of particles and for flocculation [ 44 ]. It has been proven that flocculation capacity was positively associated with uronic acid content [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment

Chen

Liu

et al. 2017

BMC Biotechnol

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BackgroundThe identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater.ResultsA bioflocculant-producing bacterium was isolated from seawater and identified as Alteromonas sp. CGMCC 10612. The optimal carbon and nitrogen sources for the strain were 30 g/L glucose and 1.5 g/L wheat flour. In a 2-L fermenter, the flocculating activity and bioflocculant yield reached maximum values of 2575.4 U/mL and 11.18 g/L, respectively. The bioflocculant was separated and showed good heat and pH stability. The purified bioflocculant was a proteoglycan consisting of 69.61% carbohydrate and 21.56% protein (wt/wt). Infrared spectrometry further indicated the presence of hydroxyl, carboxyl and amino groups preferred for flocculation. The bioflocculant was a nanoparticle polymer with an average mass of 394,000 Da. The purified bioflocculant was able to remove Congo Red, Direct Black and Methylene Blue at efficiencies of 98.5%, 97.9% and 72.3% respectively.ConclusionsThe results of this study indicated that the marine strain Alteromonas sp. is a good candidate for the production of a novel bioflocculant and suggested its potential industrial utility for biotechnological processes.Electronic supplementary materialThe online version of this article (10.1186/s12896-017-0404-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment

Chen

Liu

et al. 2017

BMC Biotechnol

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“…BDU 80591. Like EPS producing organisms, namely M. flos-aquae C3-40, Anabaena sp and Nostoc sp [15], [21] has been identified by this technique. The organisms identified based on morphology are tabulated below.…”

Section: A Morphological Identification and Negative Stainingmentioning

confidence: 99%

Identification of Potential Marine Filamentous Heterocyst Cyanobacterium producing Higher EPS Coupled with Higher Viscosity

Arulraj¹

2018

IJRASET

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Marine cyanobacterial exopolysaccharides are natural biopolymers well known for their potentials in various fields. As there is an increase in demand for a new polymer, fifteen (15) strains of different morphotypes unicellular, filamentous and filamentous heterocystous belonging to varied habitat were screened for EPS production. EPS synthesis was found to be coupled with incubation duration, growth phase, which reflected on the rheology of the medium. Filamentous heterocystous strain Nostoc sp BDU 80591 of salt pan habitat yielded (2.8 gL -1 ) EPS exhibited (4.64 cP) as viscosity.

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“…Batch cultures were prepared for characterization and optimization of bioflocculant exopolysaccharide production by cyanobacteria by the methods followed Tiwari et al [7].…”

Section: Cyanobacterial Culture Conditionsmentioning

confidence: 99%

Production of exopolysaccharides by the cyanobacterium Anabaena sp. BTA992 and application as bioflocculants

2016

J App Biol Biotech

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Bioflocculant exopolysaccharide (EPS) production by ten (10) cyanobacterial strains comprising of five (05) heterocystous and five (05) non-heterocystous strains during their photoautotrophic growth was investigated. Highest level of EPS was produced by Anabaena sp. BTA992 among the investigated strains with high flocculation capacity. The cyanobacterial EPS consisted of soluble protein and polysaccharide that included substantial amounts of neutral sugars and uronic acid. The flocculant bound a cationic d ye, alcian blue, indicating it to be polyanionic. EPS production was maximum during stationary growth phase and late phase of growth at pH 8.5. The strain was identified by 16S rRNA typing and GenBank accession number was obtained as KJ830950. The investigation revealed that this Anabaena sp. could be a potential candidate for the commercial EPS production and might be utilized in applications as an alternative to synthetic flocculants.

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Characterization and Optimization of Bioflocculant Exopolysaccharide Production by Cyanobacteria Nostoc sp. BTA97 and Anabaena sp. BTA990 in Culture Conditions

Cited by 52 publications

References 64 publications

Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment

Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment

Identification of Potential Marine Filamentous Heterocyst Cyanobacterium producing Higher EPS Coupled with Higher Viscosity

Production of exopolysaccharides by the cyanobacterium Anabaena sp. BTA992 and application as bioflocculants

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