Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane

Jochems, Peter; Satyawali, Yamini; Roy, Sandra; Doyen, W.; Diels, Ludo; Dejonghe, Winnie

doi:10.1016/j.enzmictec.2011.06.010

Cited by 41 publications

(33 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymer-brush-modified electrodes were also studied for their application in e-BES but the activity is dependent on the pH of the solution [174]. The polymeric materials are also employed to achieve additional functionalities such as receptors in the form of a polymer matrix, mediators, or as ion-selective membranes [175].…”

Section: Polymersmentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

View full text Add to dashboard Cite

Recent interest in the field of biocommodities production through bioelectrochemical systems has generated interest in the enzyme catalyzed redox reactions. Enzyme catalyzed electrodes are well established as sensors and power generators. However, a paradigm shift in recent science towards the production of useful chemicals has changed the face of biofuel cells, keeping the fuels or chemicals production in the upfront. This review article comprehensively presents the progress in the field of enzyme-electrodes for the production of electricity, fuels and chemicals with an aim to represent a practical outline for understanding the use of single or multiple redox enzymes as electrocatalysts for their electron transfer onto electrodes. It also provides the state-of-the-art information regarding the different existing processes to fabricate enzyme electrodes. Successfully-achieved electroenzymatic anodic and cathodic reactions are further discussed, together with their potential applications. Particular focus was made on the novel single/multiple enzyme systems towards product synthesis and other applications. Finally, techno-economic and environmental elements for industrial processing with enzyme catalyzed bioelectrochemical system (e-BES) are anticipated, in order to provide useful strategies for further development of this technology.

show abstract

Section: Polymersmentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

View full text Add to dashboard Cite

show abstract

“…The mixed matrix membranes were made in house and were chosen because they can be easily loaded with enzymes and operated with almost no mass transfer limitations. Furthermore, the mixed matrix membranes can be easily scaled up by adaptation of the membrane surface [3]. One issue encountered during the active site titration in case of both beads and membranes was partial adsorption of the leaving group 2 ,4 -dinitrophenolate by the supports.…”

Section: Resultsmentioning

confidence: 99%

“…Enzyme immobilization on the membrane was conducted on 1 cm 2 membrane piece by submerging it in 0.6 mL enzyme solution. Details of the immobilization have been reported previously [3]. The inhibitor 2 ,4 -dinitrophenyl 2-deoxy-2-fluoro-␤-d-galactopyranoside, used in this study, was synthesized as described before and spectra were in accordance with the literature [13,18,19].…”

Section: Enzymes and Reagentsmentioning

confidence: 99%

“…Furthermore, ␤-galactosidase is used in another large industrial process: the synthesis of galacto-oligosaccharides (GOS), which makes use of the transferase activity of the enzyme [1]. Like many other enzymes, ␤-galactosidase can also be immobilized to extract technical and economic benefits and enable its long time reuse in industrial reactors [2,3].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Active site titration of immobilized beta-galactosidase for the determination of active enzymes

Jochems

Mueller

Satyawali

et al. 2015

Biochemical Engineering Journal

Self Cite

View full text Add to dashboard Cite

“…A 1.2-fold increase in K m as a result of immobilization suggests a typical diffusion limitations increase, and the maximum specific reaction rate decreased almost by half, suggesting structural changes in the enzyme. β-galactosidase was immobilized by adsorption on a mixed-matrix membrane containing zirconium dioxide [153]. Up to 1.6 g β-galactosidase could be absorbed per m 2 of membrane.…”

Section: Other Immobilized Carbohydrasesmentioning

confidence: 99%

Potential Applications of Carbohydrases Immobilization in the Food Industry

Contesini

Figueira

Kawaguti

et al. 2013

IJMS

View full text Add to dashboard Cite

Carbohydrases find a wide application in industrial processes and products, mainly in the food industry. With these enzymes, it is possible to obtain different types of sugar syrups (viz. glucose, fructose and inverted sugar syrups), prebiotics (viz. galactooligossacharides and fructooligossacharides) and isomaltulose, which is an interesting sweetener substitute for sucrose to improve the sensory properties of juices and wines and to reduce lactose in milk. The most important carbohydrases to accomplish these goals are of microbial origin and include amylases (α-amylases and glucoamylases), invertases, inulinases, galactosidases, glucosidases, fructosyltransferases, pectinases and glucosyltransferases. Yet, for all these processes to be cost-effective for industrial application, a very efficient, simple and cheap immobilization technique is required. Immobilization techniques can involve adsorption, entrapment or covalent bonding of the enzyme into an insoluble support, or carrier-free methods, usually based on the formation of cross-linked enzyme aggregates (CLEAs). They include a broad variety of supports, such as magnetic materials, gums, gels, synthetic polymers and ionic resins. All these techniques present advantages and disadvantages and several parameters must be considered. In this work, the most recent and important studies on the immobilization of carbohydrases with potential application in the food industry are reviewed.

show abstract

Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane

Cited by 41 publications

References 42 publications

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Active site titration of immobilized beta-galactosidase for the determination of active enzymes

Potential Applications of Carbohydrases Immobilization in the Food Industry

Contact Info

Product

Resources

About