Characterization and overexpression of RHO1 from Cryptococcus laurentii ZJU10 activates CWI signaling pathway on enhancing the inhibition of blue mold on pears

Miao, Lin; Zhang, Yangyang; Sun, Cheng; Huang, Yining; Zhang, Jiahui; Zheng, Xiaodong; Yu, Ting

doi:10.1016/j.ijfoodmicro.2018.04.022

Cited by 5 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…erefore, safe and efficient antagonistic yeasts have been a research hotspot in the control of postharvest diseases of pear. Cryptococcus laurentii, Rhodotorula glutinis, Rhodosporidium paludigenum, and Rhodotorula mucilaginosa [2][3][4][5] are some effective biocontrol agents against P. expansum in pears.…”

Section: Introductionmentioning

confidence: 99%

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Yang

Dhanasekaran

Apaliya

et al. 2020

Journal of Food Quality

View full text Add to dashboard Cite

Antagonistic yeasts can inhibit fungal growth. In our previous research, Meyerozyma guilliermondii, one of the antagonistic yeasts, exhibited antagonistic activity against Penicillium expansum. However, the mechanisms, especially the molecular mechanisms of inhibiting activity of M. guilliermondii, are not clear. In this study, the protein expression profile and transcriptome characterization of P. expansum induced by M. guilliermondii were investigated. In P. expansum induced by M. guilliermondii, 66 proteins were identified as differentially expressed, among them six proteins were upregulated and 60 proteins were downregulated, which were associated with oxidative phosphorylation, ATP synthesis, basal metabolism, and response regulation. Simultaneously, a transcriptomic approach based on RNA-Seq was applied to annotate the genome of P. expansum and then studied the changes of gene expression in P. expansum treated with M. guilliermondii. The results showed that differentially expressed genes such as HEAT, Phosphoesterase, Polyketide synthase, ATPase, and Ras-association were significantly downregulated, in contrast to Cytochromes P450, Phosphatidate cytidylyltransferase, and Glutathione S-transferase, which were significantly upregulated. Interestingly, the downregulated differentially expressed proteins and genes have a corresponding relationship; these results revealed that these proteins and genes were important in the growth of P. expansum treated with M. guilliermondii.

show abstract

Section: Introductionmentioning

confidence: 99%

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Yang

Dhanasekaran

Apaliya

et al. 2020

Journal of Food Quality

View full text Add to dashboard Cite

show abstract

“…In this context, phytosanitary measures and the management of pepper cultivation should include the application of microorganisms as biocontrol agents to minimize the use of synthetic fungicides [11].Bacteria and yeasts have a high capacity to control phytopathogens without causing damage to human and animal health and the environment [12,13]. Several mechanisms have been proposed for the biocontrol of phytopathogens by bacteria and yeasts, including competition for space and nutrients [14,15], lytic enzyme production such as β-1,3-glucanase [16,17], and induction of host resistance [18,19], among others. Both microorganisms have been isolated in different terrestrial ecosystems, mainly in plants, fruits, and soils; the main bacteria used as biocontrol agents are species of Stenotrophomonas, Bacillus, and Pseudomonas, which have already been reported for the control of Fusarium proliferatum in melon [20]; Sclerotium rolfsii [21] and Rhizoctonia solani in wheat [22]; and F. solani in cassava [23], among others.…”

mentioning

confidence: 99%

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

et al. 2020

View full text Add to dashboard Cite

Ancho-type pepper (Capsicum annuum L.) is a crop susceptible to Pythium ultimum, which has already been controlled with synthetic fungicide applications; however, marine antagonist microorganisms could be an alternative source of control. The efficiency in vitro and in vivo of marine bacteria and yeasts was determined against P. ultimum. The inhibition of the radial growth of P. ultimum was quantified in vitro by the bacteria Stenotrophomonas rhizophila KM01 and KM02; Bacillus subtilis RBM01 and RBM02, B. amyloliquefaciens 2RLBF and 3R4CF; and Pseudomonas spp. 2R6BF and 2RE9CF, as well as the yeasts Debaryomyces hansenii 1R11AB, 1R11CB, and LL01 and Cryptococcus laurentii 2R3BF and 2R1CB. The β-1,3-glucanase activity of the marine microorganisms was quantified in the presence of the phytopathogen. The disease index (DI), growth parameters, and colony forming units (CFU) were determined in ancho-type pepper plants inoculated with marine bacteria, yeasts, and P. ultimum. The radial zone of the phytopathogen was inhibited by 80% and 75% by S. rhizophila KM01 and C. laurentii 2R1CB, respectively. D. hansenii LL01 and S. rhizophila KM02 showed the highest activity of β-1,3-glucanase, with 6060 U/mL and 47 U/mL, respectively. B. subtilis RBM02 protected 100% of the plants from the oomycete, and an increase was quantified in all the growth parameters and CFU. The use of these marine bacteria and yeasts are, therefore, an option for P. ultimum biocontrol in ancho-type pepper plants, thereby minimizing the application of synthetic fungicides.Agronomy 2020, 10, 408 2 of 12 by immediate infection makes management of this phytopathogen very difficult [7]. Several Pythium species, including P. aphanidermatum, P. irregular, and P. ultimum, are known to cause damping-off and root rot diseases in pepper [8][9][10]. Although synthetic fungicides have shown promising results in controlling damping-off disease, phytotoxicity and fungicide residues pose serious problems to human and animal health, as well as the environment. In this context, phytosanitary measures and the management of pepper cultivation should include the application of microorganisms as biocontrol agents to minimize the use of synthetic fungicides [11].Bacteria and yeasts have a high capacity to control phytopathogens without causing damage to human and animal health and the environment [12,13]. Several mechanisms have been proposed for the biocontrol of phytopathogens by bacteria and yeasts, including competition for space and nutrients [14,15], lytic enzyme production such as β-1,3-glucanase [16,17], and induction of host resistance [18,19], among others. Both microorganisms have been isolated in different terrestrial ecosystems, mainly in plants, fruits, and soils; the main bacteria used as biocontrol agents are species of Stenotrophomonas, Bacillus, and Pseudomonas, which have already been reported for the control of Fusarium proliferatum in melon [20]; Sclerotium rolfsii [21] and Rhizoctonia solani in wheat [22]; and F. solani in cassava [23], among others. O...

show abstract

Molecular Basis of Plant-PGPM Interactions During Amelioration of Biotic Stress

Dixit

Kamat

Srivastava

et al. 2022

Microbial Biocontrol: Food Security and Post Harvest Management

View full text Add to dashboard Cite

Characterization and overexpression of RHO1 from Cryptococcus laurentii ZJU10 activates CWI signaling pathway on enhancing the inhibition of blue mold on pears

Cited by 5 publications

References 40 publications

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

Molecular Basis of Plant-PGPM Interactions During Amelioration of Biotic Stress

Contact Info

Product

Resources

About