Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) region

Kuzmanović, Nemanja; Ivanović, Milan; Prokić, Anđelka; Gašić, Katarina; Zlatković, Nevena; Obradović, Aleksa

doi:10.1007/s10658-014-0507-5

Cited by 12 publications

(17 citation statements)

References 19 publications

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“…vitis strains (Tolba and Zaki ; Kuzmanović et al . ), all our All. vitis isolates revealed to be virulent on tomato, and this may indicate the relative genetic homogeneity of the pTi carried by our isolates.…”

Section: Discussionmentioning

confidence: 86%

“…Pathogenic All. vitis strains can direct the in planta synthesis of one or more opines Burr and Otten 1999) and they are classified using the opine synthase gene of their pTi, the most common types being octopine, nopaline, octopine/cuccumopine or vitopine (Szegedi et al 1988;Argun et al 2002;Kuzmanovi c et al 2014). In our study, only a few All.…”

Section: Discussionmentioning

confidence: 87%

“…Interestingly, next to grapevine, the isolation of All. vitis strains has only been reported on kiwi (Sawada 1992;Burr and Otten 1999;Genov et al 2006;Kuzmanovi c et al 2014;Orel et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and phylogenetic diversity of Allorhizobium vitis isolated from grapevine in Morocco

et al. 2019

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Aims Crown gall, a phytobacteriosis characterized by the formation of tumours on plant roots was observed in recently planted vineyards of the Meknes region (Morocco). The objective of this research was to analyse the diversity of pathogenic agrobacteria isolated from grapevine in Morocco. Methods and Results Eighty‐two isolates from 11 affected vineyards were characterized by recA sequencing and were found to belong to Agrobacterium tumefaciens genomospecies G1, G4 or G7, Rhizobium rhizogenes, and to Allorhizobium vitis. Only the All. vitis isolates appeared to be pathogenic on tomato and multilocus sequence analysis phylogenetic analyses revealed a weak genetic diversity, with the definition of only four genomic groups. Definition of the All. vitis genomic groups correlated with specific pathogenic traits: indeed, genomic groups differed with respect to the severity of hypersensitive response symptoms on tobacco leaves, the intensity of necrotic response on grapevine explants and opine profiles. Both vitopine and octopine were detected by UHPLC in tumours induced by isolates of three genomic groups, an opine signature scarcely ever reported. Conclusions Allorhizobium vitis is the only causative agent of crown gall on grape in Morocco, pathogenic isolates can be separated into four genomic groups. Significance and Impact of the Study This study of recently crown‐gall‐infested vineyards demonstrated that All. vitis is the only causative agent and revealed the presence of nonpathogenic Agrobacterium strain within tumours. Moreover, as the genetic diversity of the All. vitis isolates is relatively narrow, this study lays the basis for further analyses on the evolution of the disease, on the dissemination of the pTi and more globally on the fate of the different genomic groups in this newly colonized environment.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 87%

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Characterization and phylogenetic diversity of Allorhizobium vitis isolated from grapevine in Morocco

et al. 2019

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“…; Kuzmanović et al . ). Therefore, the objective of this study was to analyse genetic diversity among 54 strains of A. vitis associated with these outbreaks and to evaluate their epidemiological relationships.…”

Section: Introductionmentioning

confidence: 97%

Genetic relatedness and recombination analysis ofAllorhizobium vitisstrains associated with grapevine crown gall outbreaks in Europe

et al. 2015

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“…A. vitis is adapted to survive in the plants tissues without causing tumors until conditions becomes favorable for gall development. Therefore, the disease spreads out by asymptomatic propagating materials (Kuzmanovic et al, 2014) which is necessary to develop a reliable detection methods of the pathogen in both symptomatic and asymptomatic plant materials and in the soil to efficiently prevent the disease (Bini et al, 2008). Generally, the detection methods of A. vitis are based on their isolation on the semiselective culture media and pathogenicity tests but these methods can take many weeks for results (Johnson et al, 2013, Shams et al, 2012.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive methods for detection of Allorhizobium vitis, causal agent of grapevine crown gall

Habbadi¹,

Benbrahim²,

Benbouazza³

et al. 2017

IJEAB

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Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) region

Cited by 12 publications

References 19 publications

Characterization and phylogenetic diversity of Allorhizobium vitis isolated from grapevine in Morocco

Characterization and phylogenetic diversity of Allorhizobium vitis isolated from grapevine in Morocco

Genetic relatedness and recombination analysis ofAllorhizobium vitisstrains associated with grapevine crown gall outbreaks in Europe

Rapid and sensitive methods for detection of Allorhizobium vitis, causal agent of grapevine crown gall

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