2002
DOI: 10.1042/ba20020013
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Characterization and physicochemical properties of a lipase from Pseudomonas mendocina 3121–1

Abstract: The lipase from Pseudomonas mendocina 3121-1 was found to be homogeneous with a molecular mass of 30 kDa by SDS/PAGE. It is composed of two identical subunits. A molecular mass of 62 kDa was determined by gel chromatography on a Toyopearl HW-55F column. Some physicochemical properties of the lipase were investigated using p-nitrophenyl butyrate (p-NPB), Tween 80 solution and Sigma olive-oil emulsion as substrates. The optimum temperature was determined to be 52 degrees C with p-NPB, in the range 50-60 degrees … Show more

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Cited by 23 publications
(11 citation statements)
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“…with a temperature optima of 45 °C and 50 °C have been reported [7,13,[16][17][18]. The optimum temperature was found to be 52 °C ( p-NPB), 50-60 °C with Tween 80 and in the range 50-65 °C with olive-oil emulsion in Pseudomonas mendocina 3121-1 [19]. Lower temperature optima of 37 °C and 40 °C were reported in P. mendocina PK-12CS [12], Pseudomonas pseudomallei 12SM [10] and in P. pseudoalkaligenes F-111 [20], and still lower i.e.…”
Section: Enzyme Characteristicsmentioning
confidence: 85%
“…with a temperature optima of 45 °C and 50 °C have been reported [7,13,[16][17][18]. The optimum temperature was found to be 52 °C ( p-NPB), 50-60 °C with Tween 80 and in the range 50-65 °C with olive-oil emulsion in Pseudomonas mendocina 3121-1 [19]. Lower temperature optima of 37 °C and 40 °C were reported in P. mendocina PK-12CS [12], Pseudomonas pseudomallei 12SM [10] and in P. pseudoalkaligenes F-111 [20], and still lower i.e.…”
Section: Enzyme Characteristicsmentioning
confidence: 85%
“…Calcium ion, especially, is used in Ca +2 binding processing which impact to position specificity on active site [1]. Same lipases produced by Pseudomonas sp.…”
Section: Effect Of Metal Ions On Lipase Activitymentioning
confidence: 99%
“…The experimental values were used to calculate the half-life time of the enzyme. The pH optimum was determined over a pH range of 5-11 in 50 mM Britton and Robinson's universal buffer (Surinenaite et al 2002).…”
Section: Thermostability and Ph Optimummentioning
confidence: 99%