Characterization and quantification of humic substances 2D-Fluorescence by usage of extended size exclusion chromatography

Wagner, Martin; Schmidt, W.; Imhof, Lutz; Grübel, Anika; Jähn, Camilla; Georgi, Denise; Petzoldt, Heike

doi:10.1016/j.watres.2016.01.050

Cited by 24 publications

(7 citation statements)

References 27 publications

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“…2). Among humic substances, the large-molecule fraction dominates [28][29][30]; however, in the studied water the molecular sizes were significantly smaller than found in other studies. Additionally, it must be noticed that among dissolved organic substances, the biodegradable fraction (substances of a low molecular mass) [31] made up 14.9-31.3% of TOC.…”

Section: Resultscontrasting

confidence: 66%

Effectiveness of humic substance removal during the coagulation process

2019

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This study concerns the utility of the coagulation process in removing humic substances and its dependence on the properties of these substances and their concentrations. The coagulation process was performed on model solutions of natural humic acids. Polyaluminum chloride (PAX XL3) and aluminum sulfate were used for this study, which allowed for a comparison of the effectiveness of pre-hydrolyzed and hydrolyzing coagulants. The coagulant dosages were determined as gram aluminum per gram carbon, thanks to which it was possible to compare the process effectiveness for different initial organic carbon concentrations, whose values were in the range of 5.51-21.23 gC/m 3. The obtained values of reductions in organic carbon concentrations point to a significant process effectiveness (37.2-59.4% and 20.0-41.5% for pre-hydrolyzed and hydrolyzing coagulants, respectively), which increased with increasing molecular mass of the humic substances present in water. These results are analogous to those found for coagulation of surface waters and point to a greater effectiveness of pre-hydrolyzed coagulant in removing humic substances (at the same coagulant dosage). The effectiveness in removing organic substances increased with coagulant dosage and the initial total organic carbon concentration. The coagulation process most effectively removed aromatic substances absorbing UV light. The content of substances absorbing UV light in raw and post-coagulation water was proportional to the dissolved organic carbon content.

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Section: Resultscontrasting

confidence: 66%

Effectiveness of humic substance removal during the coagulation process

2019

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“…Thus, it is necessary to deeply excavate composition features of similar fluorescent components to distinguish these two pollution sources. Student A summarized several novel techniques that could expatiate fluorescent components, such as size exclusion chromatography and mass spectrometry. − Students B and C collectively pointed out that WQPs might assist the FF technique to determine the pollution source. Furthermore, Student C thought that the pollution source of SMTH might be the effluent of domestic wastewater in Tsinghua University, since the fluorescent components in PS3 were commonly identified in domestic wastewater, , and the TP concentration of the effluent of domestic wastewater in Tsinghua University was about 3 mg/L, which was roughly consistent with that of the SMTH water body.…”

Section: Results and Discussionmentioning

confidence: 99%

Identifying Pollution Sources in Surface Water Using a Fluorescence Fingerprint Technique in an Analytical Chemistry Laboratory Experiment for Advanced Undergraduates

Shen

Deng

2021

J. Chem. Educ.

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The undergraduates in the major of Environmental Science and Environmental Engineering frequently ignored the importance of identifying pollution sources for water environment monitoring and knew little about the techniques of identifying pollution sources. Thus, a laboratory experiment which focused on pollution sources identification in a water body was designed to help students to solve these issues. In this laboratory exercise, students collected surface water samples from a pond, namely, Shui-Mu Tsinghua (SMTH), and evaluated its water quality through water quality parameters (WQPs). Afterward, the students scanned a fluorescence fingerprint (FF) of the SMTH water body by fluorescence spectrometer and determined the suspected pollution source through parallel factor analysis and Tucker congruence coefficient. Students' performance from postlab quizzes and experimental reports implied that they understood the significance of identifying pollution sources for practical water environment monitoring. Some students presented great interest in the FF technique and proposed several suggestions to improve its ability to identify pollution sources.

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“…Size-exclusion chromatography (SEC) is one of the techniques commonly used to separate macromolecules from small molecules, and to characterise them in relation to molar mass; SEC has been employed in the study of HA properties [27][28][29], including the preparation of humic acid fractions with a definite range of particle sizes [30]. This chromatographic technique separates the analytes on the basis of their hydrodynamic volume [31,32], and therefore it permits an estimation of their molar mass.…”

Section: Size-exclusion Chromatography (Sec)mentioning

confidence: 99%

Macromolecular Structure of a Commercial Humic Acid Sample

et al. 2020

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The molecular structure of a commercial sample of humic acids (HA) was investigated by membrane dialysis experiments (MD) and low-pressure size-exclusion chromatography (LP-SEC). MD showed that HA molecules were retained by dialysis membrane with a cut-off of 6-8 kDa, independently from HA concentration (15 or 150 mg L −1 ), NaHCO 3 concentration (0.005-2.0 mol L −1 ), and from propan 2-ol (0-5 v/v %). SEC experiments at low pressure gave chromatograms with a broad peak, with an elution volume between those of the globular proteins bovine serum albumin (molecular weight = 66.5 kDa) and lysozyme from egg (molecular weight = 14.4 kDa). The pattern of the chromatogram did not vary with HA concentration, and second-run chromatograms of single eluted fractions showed relatively sharp peaks. From these data, we reveal that the commercial HA sample analysed has a macromolecular structure rather than being a supramolecular aggregate of relatively small molecules, as recently proposed for some samples of HA obtained from different sources.

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Characterization and quantification of humic substances 2D-Fluorescence by usage of extended size exclusion chromatography

Cited by 24 publications

References 27 publications

Effectiveness of humic substance removal during the coagulation process

Effectiveness of humic substance removal during the coagulation process

Identifying Pollution Sources in Surface Water Using a Fluorescence Fingerprint Technique in an Analytical Chemistry Laboratory Experiment for Advanced Undergraduates

Macromolecular Structure of a Commercial Humic Acid Sample

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