Characterization and Some Cultural Conditions of a Cyclodextrin Glycosyltransferase-producing AlkalophilicBacillussp.

Cloning and Expression of theBacillus subtilisNo. 313γ-Cyclodextrin Forming CGTase Gene inEscherichia coli

“…3 ) No enzymes which produce r-CD predominantly have been discovered yet. Recently we isolated a new bacterium (B. subtilis No.…”

A rapid screening method for alkaline .BETA.-cyclodextrin glucanotransferase using phenolphthalein-methyl orange-containing-solid medium.

“…CGTase-producing microorganisms have been isolated from soil on agar medium containing corn starch, on which clear zones were formed. [1][2] Recently, CGTaseactivity was determined on the basis of the reduction of the color intensity of phenolphthalein under alkaline conditions.5) It has also been reported that various dye molecules, such as congo red and methyl a 10 /il of each enzyme solution was spotted on 2%soluble starch medium II, followed by incubation, and then an iodine solution (0.203 g I2 +5.202g Kl/lOOml distilled water) was poured to determine the sizes of the hollow zones. b 10/4 of each enzyme solution was spotted on phenolphthalein-methyl orange medium II, followed by incubation to determine the sizes of the hollow zones.…”

Cloning and expression of the Bacillus subtilis No. 313 .GAMMA.-cyclodextrin forming CGTase gene in Escherichia coli.

“…CGTases are classified into two types, that is, B. macerans type enzymes which produce a-CD from starch2) and alkalophilic Bacillus type enzymes which produce /?-CD from starch. 3…”