Characterization, enzymatic and biochemical properties of endophytic bacterial strains of the medicinal plant Ajuga turkestanica (Rgl.) Brig (Lamiaceae)

Mamarasulov, Bahodir; Davranov, Kakhramon; Jahan, Mohammad Shah; Jabborova, Dilfuza; Nasif, Omaima; Ansari, Mohammad Javed; Danish, Subhan; Datta, Rahul

doi:10.1016/j.jksus.2022.102183

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…The nitrogen fixation by bacterial species such as Azotobacter , Clostridium , or Rhizobium has been reported to be of vital importance in plant growth [ 118 ]. Some of the genera found exclusively in the plant tissues like Pseudomonas [ 119 ], Pantoea [ 120 ], and Siccibacter [ 121 ] have been associated with nitrogen fixation and auxin biosynthesis [ 122 ]. It should be noted that rhizobacteria such as Bacillus amyloliquefaciens and Pseudomonas putida can promote the activation of the jasmonic acid and salicylate acid pathway, which increases the resistance levels to insects such as caterpillars [ 123 ].…”

Section: Discussionmentioning

confidence: 99%

Studying Plant–Insect Interactions through the Analyses of the Diversity, Composition, and Functional Inference of Their Bacteriomes

et al. 2022

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As with many other trophic interactions, the interchange of microorganisms between plants and their herbivorous insects is unavoidable. To test the hypothesis that the composition and diversity of the insect bacteriome are driven by the bacteriome of the plant, the bacteriomes of both the plant Datura inoxia and its specialist insect Lema daturaphila were characterised using 16S sRNA gene amplicon sequencing. Specifically, the bacteriomes associated with seeds, leaves, eggs, guts, and frass were described and compared. Then, the functions of the most abundant bacterial lineages found in the samples were inferred. Finally, the patterns of co-abundance among both bacteriomes were determined following a multilayer network approach. In accordance with our hypothesis, most genera were shared between plants and insects, but their abundances differed significantly within the samples collected. In the insect tissues, the most abundant genera were Pseudomonas (24.64%) in the eggs, Serratia (88.46%) in the gut, and Pseudomonas (36.27%) in the frass. In contrast, the most abundant ones in the plant were Serratia (40%) in seeds, Serratia (67%) in foliar endophytes, and Hymenobacter (12.85%) in foliar epiphytes. Indeed, PERMANOVA analysis showed that the composition of the bacteriomes was clustered by sample type (F = 9.36, p < 0.001). Functional inferences relevant to the interaction showed that in the plant samples, the category of Biosynthesis of secondary metabolites was significantly abundant (1.4%). In turn, the category of Xenobiotics degradation and metabolism was significantly present (2.5%) in the insect samples. Finally, the phyla Proteobacteria and Actinobacteriota showed a pattern of co-abundance in the insect but not in the plant, suggesting that the co-abundance and not the presence–absence patterns might be more important when studying ecological interactions.

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Section: Discussionmentioning

confidence: 99%

Studying Plant–Insect Interactions through the Analyses of the Diversity, Composition, and Functional Inference of Their Bacteriomes

et al. 2022

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“…To test the amylolytic activities of the bacteria, starch agar (1 × LB agar plus 1% starch) was used. When the bacterial incubation was over, a solution of potassium iodide was sprayed on the surfaces of the petri dishes and a colorless transparent zone formed around the bacterial colonies [ 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

Bacterial Changes in Boiled Crayfish between Different Storage Periods and Characterizations of the Specific Spoilage Bacteria

Xia

Jiang

Zhang

et al. 2023

Foods

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This study investigated changes in the microbial compositions of crayfish tails during storage at 4 °C (for 0–12 days) as measured using high-throughput sequencing (HTS). The specific spoilage organisms (SSOs) in the crayfish tails were isolated using culture-dependent cultivation methods, and they were identified by 16S rRNA and characterized for their enzymatic spoilage potentials (e.g., protease, lipase, phospholipase, and amylase). The spoilage abilities of the selected strains in the crayfish tails were assessed by inoculating them into real food. Moreover, the microbial growth and the volatile basic nitrogen (TVB-N) changes were monitored during the storage period. The results from the HTS showed that the dominant genus of shrimp tails evolved from Streptococcus (D0) to Pseudomonas (D4) and, finally, to Paenisporosarcina (D12) during storage. Seven bacterial species (Acinetobacter lwoffii, Aeromonas veronii, Kurthia gibsonii, Pseudomonas sp., Exiguobacterium aurantiacum, Lelliottia amnigena, and Citrobacter freundii) were screened from the spoiled shrimp tails by the culture-dependent method, among which Aeromonas veronii had the strongest spoilage ability.

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Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Parasuraman,

Busi,

Lee

2024

ESKAPE Pathogens

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Characterization, enzymatic and biochemical properties of endophytic bacterial strains of the medicinal plant Ajuga turkestanica (Rgl.) Brig (Lamiaceae)

Cited by 7 publications

References 32 publications

Studying Plant–Insect Interactions through the Analyses of the Diversity, Composition, and Functional Inference of Their Bacteriomes

Studying Plant–Insect Interactions through the Analyses of the Diversity, Composition, and Functional Inference of Their Bacteriomes

Bacterial Changes in Boiled Crayfish between Different Storage Periods and Characterizations of the Specific Spoilage Bacteria

Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

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