Characterization, expression, and response to stress of p8 gene in amphioxus

Liu, Zhenhui; Sun, Yong; Liu, Naiguo; Fan, Ningning; Zhang, Shicui

doi:10.1016/j.fsi.2009.06.011

Cited by 8 publications

(11 citation statements)

References 22 publications

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“…For example, p8 is both proapoptotic [8,9] and antiapoptotic [10,11]. Expression of p8 is up-regulated by stressors including lipopolysaccharide (LPS) and simply changing culture media [4,6,12]. It is also involved in the regulation of cellular growth [1,2], cellular growth inhibition [8,13], tumor development and progression [7,14e16], animal embryo development [1,17] and cell defence [18].…”

Section: Introductionmentioning

confidence: 99%

Tissue distribution, developmental expression and up-regulation of p8 transcripts on stress in zebrafish

Sun

Liu

Zhang

2010

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 99%

Tissue distribution, developmental expression and up-regulation of p8 transcripts on stress in zebrafish

Sun

Liu

Zhang

2010

Fish & Shellfish Immunology

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“…Previously we have shown that amphioxus p8 transcripts were up-regulated significantly on LPS and during starvation challenge trials [8], suggesting that it is a stress-related gene. Further experiments also demonstrated that zebrafish p8 respond to a wider range of cellular stresses such as temperature, osmotic pressure and pH [11].…”

Section: Introductionmentioning

confidence: 88%

“…Homologs of the p8 gene have subsequently been cloned and sequenced in both humans and mice [2,3]. This gene is conserved in mammals such as mice and cattle [1,2,4], but also in a wide variety of other organisms including brine shrimp [5], fruit flies [6], sea urchins [7], amphioxus [8], frogs [9], Atlantic salmon [10] and zebrafish [11]. Human p8 (Hp8) encodes an 82 amino acid polypeptide, which contains a basic helix-loop-helix (bHLH) motif and a canonical bipartite nuclear-targeting signal (NLS) which promotes nuclear targeting.…”

Section: Introductionmentioning

confidence: 99%

Localization, developmental regulation and stress induction of p8-L1 and p8-L2 in zebrafish

et al. 2012

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The transcription cofactor, p8, has a basic helix-loop-helix motif and is involved in several metabolic processes. It has previously been reported that p8 plays an important role in stress response in zebrafish. We expanded on these studies to further investigate the roles of two p8-like cDNAs found in zerbrafish, p8-L1 and p8-L2. Zebrafish p8-L1 and p8-L2 cDNAs consist of 742 bp and 813 bp, respectively. Both have the same open reading frame despite different genomic structures and encode a 76 amino acid polypeptide with conserved a Phospho_p8 domain. These two cDNA’s were abundant in different tissues in the zebrafish: p8-L1 was high in intestine and muscle tissue and in low abundance in the backbone, whereas the highest amount of p8-L2 was found in the backbone, similar to p8. During embryogenesis, both p8-L1 and p8-L2 were abundant at the cleavage stage and decreased gradually in abundance from blastula to pharyngula stage. However, p8-L1 abundance increased during hatching as observed in p8. Quantitative real-time PCR assay suggested that p8-L1 and p8-L2 were both up-regulated significantly under osmotic pressure and pH value challenges, suggesting an important role of p8-L1 and p8-L2 genes in stress response.

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“…The p8 gene is also structurally similar to the metastasis-1 gene implicated in human breast cancer (Ree et al 1999). Homologs of p8 have subsequently been cloned and characterized in both vertebrates and invertebrates, such as humans, rats, mice, frogs, amphioxus, fruit flies, and shrimp (Vasseur et al 1999a, b;Mallo et al 1997;Igarashi et al 2001;Encinar et al 2001;Liu et al 2009;Zinke et al 2002;Qiu and MacRae 2007). Other than Atlantic salmon (Genbank accession no.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of p8 Homolog cDNA in the Atlantic Halibut (Hippoglossus hippoglossus)

Wang

Lin

et al. 2010

Biochem Genet

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The p8 gene encodes a transcription factor known to modulate cell growth, division, and apoptosis and influences gene expression. In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 3'UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules.

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Characterization, expression, and response to stress of p8 gene in amphioxus

Cited by 8 publications

References 22 publications

Tissue distribution, developmental expression and up-regulation of p8 transcripts on stress in zebrafish

Tissue distribution, developmental expression and up-regulation of p8 transcripts on stress in zebrafish

Localization, developmental regulation and stress induction of p8-L1 and p8-L2 in zebrafish

Cloning and Characterization of p8 Homolog cDNA in the Atlantic Halibut (Hippoglossus hippoglossus)

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