Characterization, immobilization, and activity enhancement of cellulase treated with supercritical CO2

Senyay-Oncel, Deniz; Yeşil-Çeliktaş, Özlem

doi:10.1007/s10570-015-0780-2

Cited by 20 publications

(2 citation statements)

References 25 publications

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“…These findings are in agreement with the data on thermal and chemical stability, which show a significant improvement in the stability of the cellulase after immobilization. The foregoing observations are in agreement with data published by Senyay-Oncel and Yesil-Celiktas, which showed that after the immobilization of cellulase on commercially available NaY zeolite, its substrate affinity and reaction rate also decreased [44]. Nonetheless, their study found much more significant changes in K m (a 100% rise), V max (a 30% drop), and k cat (a 50% drop) than in the present study.…”

Section: Kinetic Parameters Of Free and Immobilized Cellulasesupporting

confidence: 94%

Immobilization of Cellulase on a Functional Inorganic–Organic Hybrid Support: Stability and Kinetic Study

et al. 2017

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Abstract:Cellulase from Aspergillus niger was immobilized on a synthesized TiO 2 -lignin hybrid support. The enzyme was effectively deposited on the inorganic-organic hybrid matrix, mainly via physical interactions. The optimal initial immobilization parameters, selected for the highest relative activity, were pH 5.0, 6 h process duration, and an enzyme solution concentration of 5 mg/mL. Moreover, the effects of pH, temperature, and number of consecutive catalytic cycles and the storage stability of free and immobilized cellulase were evaluated and compared. Thermal and chemical stability were significantly improved, while after 3 h at a temperature of 50 • C and pH 6.0, the immobilized cellulase retained over 80% of its initial activity. In addition, the half-life of the immobilized cellulase (307 min) was five times that of the free enzyme (63 min). After ten repeated catalytic cycles, the immobilized biocatalyst retained over 90% of its initial catalytic properties. This study presents a protocol for the production of highly stable and reusable biocatalytic systems for practical application in the hydrolysis of cellulose.

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Section: Kinetic Parameters Of Free and Immobilized Cellulasesupporting

confidence: 94%

Immobilization of Cellulase on a Functional Inorganic–Organic Hybrid Support: Stability and Kinetic Study

et al. 2017

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“…Supercritical CO 2 is a green solvent, and it is widely used in supercritical fiber dyeing process [1][2][3][4]. Senyay-Oncel and Yesil-Celiktas [5] studied activity enhancement of cellulose treated with supercritical CO 2 , and found that enzymes as catalysts in biochemical applications can be greatly improved by using supercritical fluids as potential media. Russler et al [6] studied different approaches towards hydrophobic modification of bacterial cellulose aerogels with the alkyl ketene dimer (AKD) reagent, and found that if AKD modification was performed in supercritical CO 2 , an unexpectedly high degree of loading was observed.…”

Section: Introductionmentioning

confidence: 99%