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Utilization of renewable resources and development of new processes aimed at converting these materials into value added bio-products are gaining more emphasis. The scope of this study was to optimize supercritical CO 2 extraction (SFE) parameters such as pressure, temperature and flow rate for the yields of unsaturated fatty acids from Pistacia terebinthus berries by a Box-Behnken statistical design. All samples were analyzed for fatty acids by GC-FID. The most effective variables were pressure (P \ 0.005) and flow rate (P B 0.005). Maximizing the evaluative criteria for dependent variables (oleic acid, linoleic acid and linolenic acid), optimal conditions were determined to be 240 bar, 60°C and a flow rate of 16 g/min yielding 51.2% oleic acid, 26.5% linoleic acid and 1.0% linolenic acid. The amounts of unsaturated fatty acids in SFE samples (81.3%) were higher than the hexane (74.3%) and were similar to that of cold press samples (80.1%). High concentrations of unsaturated fatty acids can indicate the utilization of the berries as a major dietary source and demonstrate challenges for industrial application of SFE as a green technology.

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