Processing of protease under sub- and supercritical conditions for activity and stability enhancement

Senyay-Oncel, Deniz; Kazan, Aslihan; Yeşil-Çeliktaş, Özlem

doi:10.1016/j.bej.2014.06.021

Cited by 13 publications

(3 citation statements)

References 25 publications

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“…In addition to conventional physical methods, supercritical carbon dioxide (SC-CO 2 ) has been proposed as a promising method for decellularisation by means of bursting the cells with high pressure and removing nuclear fragments from the tissues (Refs 54 , 55 ). As the critical pressure (7.4 MPa) and temperature (31 °C) of CO 2 to reach supercritical phase are relatively mild, processing of various biological materials has been reported (Refs 56 , 57 , 58 ). SC-CO 2 shows the properties of both liquids and gases, diffuse through solids like a gas and dissolve substances like liquid allowing a low viscosity and high transport characteristics for simple and short decellularisation protocols.…”

Section: Decellularisation Methodsmentioning

confidence: 99%

Decellularised extracellular matrix-based biomaterials for repair and regeneration of central nervous system

Yaldiz

Sağlam-Metiner

Yeşil-Çeliktaş

2021

Expert Rev. Mol. Med.

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The central nervous system (CNS), consisting of the brain and spinal cord, regulates the mind and functions of the organs. CNS diseases, leading to changes in neurological functions in corresponding sites and causing long-term disability, represent one of the major public health issues with significant clinical and economic burdens worldwide. In particular, the abnormal changes in the extracellular matrix under various disease conditions have been demonstrated as one of the main factors that can alter normal cell function and reduce the neuroregeneration potential in damaged tissue. Decellularised extracellular matrix (dECM)-based biomaterials have been recently utilised for CNS applications, closely mimicking the native tissue. dECM retains tissue-specific components, including proteoglycan as well as structural and functional proteins. Due to their unique composition, these biomaterials can stimulate sensitive repair mechanisms associated with CNS damages. Herein, we discuss the decellularisation of the brain and spinal cord as well as recellularisation of acellular matrix and the recent progress in the utilisation of brain and spinal cord dECM.

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Section: Decellularisation Methodsmentioning

confidence: 99%

Decellularised extracellular matrix-based biomaterials for repair and regeneration of central nervous system

Yaldiz

Sağlam-Metiner

Yeşil-Çeliktaş

2021

Expert Rev. Mol. Med.

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“…The method of evaluating the protease activity in glutinous rice dough samples was described by Senyay-Oncel et al (2014). Dough samples were dissolved in phosphate buffer (1: 10, w/v), stirred, and then centrifuged (5,000 g, 10 min).…”

Section: Determination Of Protease Activitymentioning

confidence: 99%

Casein hydrolysate's effects on the fermentation properties, texture, and chemical characteristics and the bacterial microbiota of fermented glutinous rice dough

Guo,

Liu,

Jiang

et al. 2023

Front. Microbiol.

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To investigate how casein hydrolysate affected the physicochemical properties and microbiological diversity of the glutinous rice dough (natural fermentation and yeast fermentation), we analyzed its fermentation properties, carbohydrate, protein degradation, texture, and bacterial composition. According to the findings, casein hydrolysate increased the total LAB number, as well as organic acid content, in naturally fermented and yeast fermented glutinous rice dough by 3.59 and 8.19%, respectively, and reduced the fermentation time by at least 2 h. Meanwhile, casein hydrolysate enhanced the content of reducing sugars by 4.46 and 13.53% and increased protease activity by 29.9 and 27.7%. In addition, casein hydrolysate accelerated protein breakdown and regulated the hardness of the dough to improve the texture. Casein hydrolysate enriched the bacterial richness and diversity of dough. After adding casein hydrolysate, it promoted the growth of Pediococcus, Enterococcus, Lactobacillus, and Streptococcus. According to the Spearman correlation analysis, environmental factors (pH, lactic acid, acetic acid, reducing sugar content, and protease activity) exhibited the major driver for the abundance of bacterial species (Spearman correlation coefficient: −0.71 to 0.78). As a potential food additive, casein hydrolysate can improve the fermentation and quality of glutinous rice dough, increase consumer acceptance of cereal foods, and give consumers healthier options.

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“…Protease activity of Jiu-niang was conducted by the hydrolysis of 1% casein at 40 C for 10 min with the Folin method. 19 1 mL Jiu-niang was incubated with 1 mL substrate solution for 10 min. 2 mL trichloroacetic acid was added in order to stop the reaction.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Use of fermented glutinous rice as a natural enzyme cocktail for improving dough quality and bread staling

Chen

Rui

et al. 2017

RSC Adv.

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Processing of protease under sub- and supercritical conditions for activity and stability enhancement

Cited by 13 publications

References 25 publications

Decellularised extracellular matrix-based biomaterials for repair and regeneration of central nervous system

Decellularised extracellular matrix-based biomaterials for repair and regeneration of central nervous system

Casein hydrolysate's effects on the fermentation properties, texture, and chemical characteristics and the bacterial microbiota of fermented glutinous rice dough

Use of fermented glutinous rice as a natural enzyme cocktail for improving dough quality and bread staling

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