2005
DOI: 10.1186/1297-9686-37-5-455
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Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds

Abstract: In order to study duck microsatellites, we constructed a library enriched for (CA)n, (CAG)n, (GCC)n and (TTTC)n. A total of 35 pairs of primers from these microsatellites were developed and used to detect polymorphisms in 31 unrelated Peking ducks. Twenty-eight loci were polymorphic and seven loci were monomorphic. A total of 117 alleles were observed from these polymorphic microsatellite markers, which ranged from 2 to 14 with an average of 4.18 per locus. The frequencies of the 117 alleles ranged from 0.02 t… Show more

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Cited by 53 publications
(60 citation statements)
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“…Sequencing of clones and designing of primers: A library enriched for (CA) n , (CAG) n , (GCC) n , and (TTTC) n was constructed from a female Peking duck (Huang et al 2005). Plasmids, extracted from clones of the microsatellite-enriched library, were sequenced with the BigDye kit on PRISM 377 DNA sequencers (ABI, Columbia, MD).…”
Section: Methodsmentioning
confidence: 99%
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“…Sequencing of clones and designing of primers: A library enriched for (CA) n , (CAG) n , (GCC) n , and (TTTC) n was constructed from a female Peking duck (Huang et al 2005). Plasmids, extracted from clones of the microsatellite-enriched library, were sequenced with the BigDye kit on PRISM 377 DNA sequencers (ABI, Columbia, MD).…”
Section: Methodsmentioning
confidence: 99%
“…To isolate the specific microsatellite markers for duck, a total of 1025 plasmids, extracted from the microsatelliteenriched library (Huang et al 2005), were sequenced according to the procedures described in materials and methods; 753 (73.46%) were found to contain (CA) n , (CAG) n , (GCC) n , (TTTC) n , (TC) n , (TCC) n , (TTCC) n , (TTTTC) n , (TTGCC) n , (TTCCTC) n , (AAC) n , (AAAC) n , (TA) n , or (TTTA) n microsatellites with five or more repeats, and 5 contained (TCTCTTTC) n , (TTTCCCTCTTTC) n , or (CTTCTTTC) n with six or more repeats. Of the 758 sequences, 539 (71.11%) contained .12 bp of uninterrupted core repeats.…”
Section: Isolation and Characterization Of Microsatellite Locimentioning
confidence: 99%
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