Characterization of 40,000- and 25,000-dalton intermediate precursors to Rauscher murine leukemia virus gag gene products

Naso, Robert; Karshin, W. L.; Wu, Yuanyuan; Arlinghaus, Ralph B.

doi:10.1128/jvi.32.1.187-198.1979

Cited by 43 publications

(27 citation statements)

References 28 publications

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“…Referring to the domains defined in MoMLV, the conserved sequences in Figure 2 lie within the CA domain. The CA protein of MoMLV is ∼30 kDa, spanning 263 amino acids, and forms the hydrophobic internal core of the viral virion (32,33). The MoMLV sequence corresponding to amino acids 27-217 in the p30 protein (amino acids 243-433 in the MoMLV gag protein) is represented in Figure 2, demonstrating that similarity between MoMLV and chromoviruses can be recognized in most parts of the p30 protein.…”

Section: Comparative Analysis Of the Gag Protein Among 'Chromovirusesmentioning

confidence: 97%

Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

Nakayashiki

Matsuo²,

Chuma³

et al. 2001

Nucleic Acids Research

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A novel Ty3/Gypsy retrotransposon, named Pyret, was identified in the plant pathogenic fungus Magnaporthe grisea (anamorph Pyricularia oryzae). Pyret-related elements were distributed in a wide range of Pyricularia isolates from various gramineous plants. The Pyret element is 7250 bp in length with a 475 bp LTR and one conceptual ORF. The ORF contains seven nonsense mutations in the reading frame, indicating that the Pyret clone is lightly degenerate. Comparative domain analysis among retroelements revealed that Pyret exhibits an extra domain (WCCH domain) beyond the basic components of LTR retrotransposons. The WCCH domain consists of approximately 300 amino acids and is located downstream of the nucleocapsid domain. The WCCH domain is so named because it contains two repeats of a characteristic amino acid sequence, W-X(2)-C-X(4)-C-X(2)-H-X(3)-K. A WCCH motif-like sequence is found in the precoat protein of some geminiviruses, viral RNA-dependent RNA polymerase and also in an Arabidopsis protein of unknown function. Interestingly, detailed sequence analysis of the gag protein revealed that Pyret, as well as some other chromodomain-containing LTR retrotransposons, displays significant sequence homology with members of the gammaretroviruses (MLV-related retroviruses) in the capsid and nucleocapsid domains. This suggests that chromodomain-containing LTR retrotransposons and gammaretroviruses may share a common ancestor with the gag protein.

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Section: Comparative Analysis Of the Gag Protein Among 'Chromovirusesmentioning

confidence: 97%

Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

Nakayashiki

Matsuo²,

Chuma³

et al. 2001

Nucleic Acids Research

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“…It most likely represents a partially cleaved gag product. Such a 40-kDa gag intermediate containing p30 and plO determinants has already been identified in MuLV (33). This p4W'ag' intermediate most likely arises from the helper Pr659"-' precursor.…”

Section: Discussionmentioning

confidence: 72%

Characterization of the gag/fusion protein encoded by the defective Duplan retrovirus inducing murine acquired immunodeficiency syndrome

Huang

Jolicoeur

1990

J Virol

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“…Anti-p30 (lane 3), anti-p 12 (lane 4), anti-p 15 (lane 5), and anti-p 10 (not shown) precipitate p36 in different amounts, which may reflect antibody titer or avidity differences. As observed by others (28), the heteroantisera reactive with p 10, p 12, and p 15 may be contaminated with low levels of antibody to p30, therefore, the precise antigenic determinants present on p36 are ambiguous. However, the observation that anti-pl2 (lane 4)precipitates larger amounts of p36 than of p30 suggests that its reactivity with p36 is not due solely to these contaminating anti-p30 antibodies.…”

Section: Identification Of a Novel Gag-related Protein P36 In Radlv-imentioning

confidence: 81%

Identification of a 36,000-molecular weight, gag-related phosphoprotein in lymphoma cells transformed by radiation leukemia virus.

Bach¹,

Meruelo²

1984

The Journal of Experimental Medicine

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Radiation leukemia virus (RadLV) causes thymic lymphoma in 90% of susceptible mice after a latent period of several months. The virally encoded polypeptides produced by RadLV-induced lymphoma cells were analyzed by immunoprecipitation and NaDodSO4/polyacrylamide gel electrophoresis. Along with the expected precursor and mature forms of gag and env gene products, a polypeptide of 36,000 molecular weight (p36) was precipitated by anti-gag antisera. It was not precipitable by normal sera or anti-env antibodies. Like the gag-associated fusion proteins of some acute leukemia viruses, p36 was found to be phosphorylated in vivo, although it lacked detectable ATP-specific protein kinase activity in vitro. By kinetics during pulse-chase labeling experiments and by comparison of two-dimensional tryptic peptide maps, this protein is not an intermediate in gag precursor processing. One lymphoma cell line is described that resembles a nonproducer RadLV-transformant, synthesizing relatively large amounts of p36 in the absence of Pr66gag or p30 production. Several RadLV-induced lymphoma cell lines also produce p36, while it was not detectable in the radiation-induced lines tested. In addition, p36 was not produced by mouse or mink fibroblasts or cultured thymocyte cell lines infected with virus passaged from the RadLV-induced lymphomas. We conclude that p36 may represent a previously unrecognized transformation-related protein induced directly or indirectly by infection with RadLV.

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Characterization of 40,000- and 25,000-dalton intermediate precursors to Rauscher murine leukemia virus gag gene products

Cited by 43 publications

References 28 publications

Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

Characterization of the gag/fusion protein encoded by the defective Duplan retrovirus inducing murine acquired immunodeficiency syndrome

Identification of a 36,000-molecular weight, gag-related phosphoprotein in lymphoma cells transformed by radiation leukemia virus.

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