Characterization of a Bacteriocin-Like Substance Produced by a Vaginal
            <i>Lactobacillus salivarius</i>
            Strain

Ocaña, Virginia S.; Holgado, Aı́da A. Pesce de Ruiz; Nader-Macı́as, Marı́a Elena

doi:10.1128/aem.65.12.5631-5635.1999

Cited by 142 publications

(94 citation statements)

References 22 publications

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“…Consistent with this hypothesis, Niku-Paavola et al (1999) have proposed that compounds secreted by Lactobacillus plantarum act synergistically with lactic acid, and Makras et al (2006) observed that L. johnsonii NCC533 CFCS was effective against serovar Typhimurium by unknown inhibitory substance(s) that are only active in the presence of lactic acid. These nonlactic acid, heat-resistant anti-Salmonella molecule(s) present in the CFCSs of probiotic Lactobacillus strains have not yet been identified (McGroarty & Reid, 1988;Bernet-Camard et al, 1997;Coconnier et al, 1997;Hudault et al, 1997;Ocana et al, 1999;Aroutcheva et al, 2001b;van de Guchte et al, 2001;Sgouras et al, 2004Sgouras et al, , 2005Fayol-Messaoudi et al, 2005Atassi et al, 2006a). It has already been suggested that pyroglutamic acid may be responsible for the antimicrobial activity of L. rhamnosus GG and L. casei strains LC-10 and LB1931 (Silva et al, 1987;Huttunen et al, 1995;Yang et al, 1997), but it has been found to be intrinsically present in MRS medium and it does not increase during bacterial growth (De Keersmaecker et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathog

Atassi¹,

Servin²

2010

FEMS Microbiology Letters

109

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The mechanism underlying the killing activity of Lactobacillus strains against bacterial pathogens appears to be multifactorial. Here, we investigate the respective contributions of hydrogen peroxide and lactic acid in killing bacterial pathogens associated with the human vagina, urinary tract or intestine by two hydrogen peroxide-producing strains. In co-culture, the human intestinal strain Lactobacillus johnsonii NCC933 and human vaginal strain Lactobacillus gasseri KS120.1 strains killed enteric Salmonella enterica serovar Typhimurium SL1344, vaginal Gardnerella vaginalis DSM 4944 and urinary tract Escherichia coli CFT073 pathogens. The cell-free culture supernatants (CFCSs) produced the same reduction in SL1344, DSM 4944 and CFT073 viability, whereas isolated bacteria had no effect. The killing activity of CFCSs was heat-stable. In the presence of Dulbecco's modified Eagle's minimum essential medium inhibiting the lactic acid-dependent killing activity, CFCSs were less effective at killing of the pathogens. Catalase-treated CFCSs displayed a strong decreased activity. Tested alone, hydrogen peroxide triggered a concentration-dependent killing activity against all three pathogens. Lactic acid alone developed a killing activity only at concentrations higher than that present in CFCSs. In the presence of lactic acid at a concentration present in Lactobacillus CFCSs, hydrogen peroxide displayed enhanced killing activity. Collectively, these results demonstrate that for hydrogen peroxide-producing Lactobacillus strains, the main metabolites of Lactobacillus, lactic acid and hydrogen peroxide, act co-operatively to kill enteric, vaginosis-associated and uropathogenic pathogens.

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Section: Discussionmentioning

confidence: 99%

Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathog

Atassi¹,

Servin²

2010

FEMS Microbiology Letters

109

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“…Inhibition of pathogen growth is a property often displayed by lactic acid strains (Vandenbergh 1993). Pathogen growth has been found to be inhibited as the result of the production of inhibiting factors by Lactobacillus strains, including bacteriocin-like substances, hydrogen peroxide and/or nonbacteriocin, nonlactic acid molecules (McGroarty and Reid 1988;Klebanoff et al 1991;Ocana et al 1999;Aroutcheva et al 2001;van de Guchte et al 2001). The data show that after direct co-culture with L. helveticus strain KS300 the viability of vaginosis-associated G. vaginalis, P. bivia, diarrhoeagenic S. typhimurium, and uropathogenic Dr-positive E. coli was reduced.…”

Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activity of Lactobacillus helveticus strain KS300 against diarrhoeagenic, uropathogenic and vaginosis-associated bacteria

Atassi

Brassart²,

Grob³

et al. 2006

J Appl Microbiol

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Aims: The purpose of this study was to investigate in vitro the antibacterial activity of the Lactobacillus helveticus strain KS300 against vaginosis‐associated bacteria including Gardnerella vaginalis and Prevotella bivia, uropathogenic Escherichia coli, and diarrhoeagenic Salmonella enterica serovar Typhimurium. Methods and Results: The KS300 strain inhibited the growth of G. vaginalis, P. bivia, S. typhimurium, and pathogenic E. coli. After direct co‐culture, data show that the Lactobacillus strain decreased the viability of G. vaginalis, P. bivia, S. typhimurium, and pathogenic E. coli. The adhering KS300 strain inhibited the adhesion of G. vaginalis DSM 4944 and uropathogenic Dr‐positive E. coli IH11128 onto HeLa cells. Moreover, the KS300 strain inhibited the internalization of uropathogenic Dr‐positive E. coli IH11128 within HeLa cells and S. typhimurium SL1344 within Caco‐2/TC7 cells. Conclusions: The findings demonstrate that L. helveticus strain KS300 is adhesive onto cultured human cells and has antagonistic activities against vaginosis‐associated, uropathogenic and diarrhoeagenic pathogens. Significance and Impact of the Study: Adhering L. helveticus strain KS300 is a potential probiotic strain displaying a strain‐specific array of in vitro antibacterial activities.

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“…Our research group has isolated and identified lactic acid bacteria from women in San Miguel de Tucum an (Argentina) (Ocaña et al 1999a;Ju arez Tom as et al 2011) and evaluated their beneficial properties that include the production of antimicrobial substances (lactic acid, hydrogen peroxide and bacteriocins) (Ocaña et al 1999b(Ocaña et al , 1999c(Ocaña et al , 1999dVera Pingitore et al 2009;Ju arez Tom as et al 2011;Nader-Mac ıas and Ju arez-Tom as 2015), biofilm formation (Leccese Terraf et al 2016Terraf et al , 2017, inhibition of urogenital pathogens (e.g. Candida albicans, Streptococcus agalactiae, Escherichia coli, Staphylococcus aureus), modulation of the immune system and protection against pathogens in experimental murine model (De Gregorio et al 2014; Nader-Mac ıas and Ju arez-Tom as 2015; De Gregorio et al 2016;De Gregorio et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Technological characterization of vaginal probiotic lactobacilli: resistance to osmotic stress and strains compatibility

et al. 2019

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Aims The aim was to evaluate the osmotic stress resistance of vaginal beneficial probiotic strains, their growth kinetics and parameters when growing in salt‐added culture media, and their compatibility to go further in the design of a probiotic formula for reconstitution of vaginal microbiome in women. Methods and Results The resistance to osmotic stress of the lactobacilli was evaluated by determining their growth in MRS (as control) added with NaCl (2–8%). The most resistant strains were Lactobacillus gasseri CRL1509, L. rhamnosus CRL1332 and L. reuteri CRL1327 selected by statistical approaches and growth parameters. Electron microscopy was applied to determine changes. They maintain probiotic properties and viability. Some strains showed incompatibility, then they cannot be included in multistrain formulas. Conclusions The resistance to different salt concentrations in vaginal lactobacilli is strain‐specific, because the behaviour is different in strains identified into the same species. The resistance is not related to the metabolic groups. Significance and Impact of the Study The resistance and survival to extreme osmotic resistance is one of the specific requirements of beneficial bacteria after the technological processes for their inclusion in probiotic formulas, in a way to express their beneficial characteristics and exert the effect on the host.

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Characterization of a Bacteriocin-Like Substance Produced by a Vaginal Lactobacillus salivarius Strain

Cited by 142 publications

References 22 publications

Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathog

Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathog

In vitro antibacterial activity of Lactobacillus helveticus strain KS300 against diarrhoeagenic, uropathogenic and vaginosis-associated bacteria

Technological characterization of vaginal probiotic lactobacilli: resistance to osmotic stress and strains compatibility

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