Characterization of a begomovirus causing horsegram yellow mosaic disease in India

Barnabas, Anburaj Daniel; Radhakrishnan, Girish; Usha, R.

doi:10.1007/s10658-009-9569-1

Cited by 24 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the natural hosts like bhendi, horsegram, and soybean are not efficiently infected by the infectious viral clones by agroinfection as reported for Bhendi yellow vein mosaic virus [22], Horsegram yellow mosaic virus [23], and the soybean isolate of Mungbean yellow mosaic India virus [24]. The method reported in the present case showed up to 83% infection in the host V. cinerea with the infectious clones of VeYVV.…”

Section: Discussionsupporting

confidence: 48%

A simple and efficient method for agroinfection of Vernonia cinerea with infectious clones of Vernonia yellow vein virus

Packialakshmi

Usha

2011

Virus Genes

View full text Add to dashboard Cite

Vernonia yellow vein virus (VeYVV) is a distinct monopartite begomovirus associated with a satellite DNA β. After constructing dimers of both DNA A and DNA β in binary vectors, a number of infection methods were attempted. However, only a modified stem-prick method produced up to 83% infection in the natural host Vernonia cinerea, thus, fulfilling the Koch's postulate. The presence of the viral DNA in the agroinfected plants was confirmed by rolling circle amplification (RCA), followed by Southern hybridization. DNA β induces typical symptoms of Vernonia yellow vein disease (VeYVD) when co-agroinoculated with the begomovirus to Vernonia and also leads to the accumulation of DNA A systemically. VeYVV represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction.

show abstract

Section: Discussionsupporting

confidence: 48%

A simple and efficient method for agroinfection of Vernonia cinerea with infectious clones of Vernonia yellow vein virus

Packialakshmi

Usha

2011

Virus Genes

View full text Add to dashboard Cite

show abstract

“…Isolates were selected based on crop, collection location and sequence of the 1.5 kb DNA‐A PCR product for full‐length sequencing. Based on the alignment of the 1.5 kb PCR products and nucleotide sequences obtained from GenBank (Table S1; Alabi et al , ; Barnabas et al , ; Blair et al , ; Durham et al , , Fernandes et al , ; Gharsallah Chouchane et al , ; Gilbertson et al , ; Hameed & Robinson, ; Morinaga et al , ; Pant et al , ; Rouhibakhsh et al , ; Singh et al , ; Usharani et al , ; Yadav et al , ; Zaim et al , ), abutting primer pairs were designed to amplify the full‐length DNA‐As and DNA‐Bs of MYMIV and MYMV isolates from Indonesia and Vietnam, respectively (Table S2). Full‐length viral DNA‐A and DNA‐B were amplified from each selected sample by PCR as previously described (Tsai et al , 2011 b ).…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

Tsai

Shih

Rauf

et al. 2013

Annals of Applied Biology

View full text Add to dashboard Cite

High incidences of yellow mosaic symptoms were observed in soybean and yard-long bean crops in Indonesia in 2009 and in mungbean crops in Vietnam in 2011. All five soybean and 20 yard-long bean samples from Java, Indonesia, and 15 mungbean samples from Vietnam with symptoms tested positive for begomovirus infection by polymerase chain reaction (PCR) with primer pair PAL1v1978B/PAR1c715H. On the basis of collection location and the nucleotide sequence comparisons of the 1.5 kb begomoviral DNA-A components amplified, a subset of samples comprising two soybean and six yard-long bean isolates from Indonesia and five mungbean isolates from Vietnam were taken forward for more detailed examination. Sequence comparison and phylogenetic analysis of the full-length sequences of all Indonesian and Vietnam isolates alongside other legume-infecting begomoviruses revealed that all the isolates from Indonesia were Mungbean yellow mosaic India virus (MYMIV) strain-A, and all from Vietnam were Mungbean yellow mosaic virus (MYMV) strain-B. To the best of our knowledge, this is the first identification of MYMIV and MYMV associated with yellow mosaic of legumes in Indonesia and Vietnam, respectively. The epidemiological implications and potential consequences of the emergence of legume-infecting begomoviruses on legume production in these areas of Southeast Asia are discussed.

show abstract

“…In addition to horse gram, HgYMV infects French bean, groundnut, lima bean, mungbean, pigeonpea, soybean and bambara groundnut in India. HgYMV was identified as a distinct species of Old World bipartite begomoviruses (Barnabas, Radhakrishnan, & Ramakrishnan, 2010). Indigofera hirsuta, a legume weed, was shown to serve as a natural reservoir of HgYMV.…”

Section: Horse Grammentioning

confidence: 99%

Tropical Food Legumes

Hema

Sreenivasulu

Patil

et al. 2014

Advances in Virus Research

View full text Add to dashboard Cite

Characterization of a begomovirus causing horsegram yellow mosaic disease in India

Abstract: The virus causing horsegram (Macrotyloma

Cited by 24 publications

References 17 publications

A simple and efficient method for agroinfection of Vernonia cinerea with infectious clones of Vernonia yellow vein virus

A simple and efficient method for agroinfection of Vernonia cinerea with infectious clones of Vernonia yellow vein virus

Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

Tropical Food Legumes

Contact Info

Product

Resources

About