Characterization of a Bioactive Acyclotide from <i>Palicourea rigida</i>

Pinto, Michelle Flaviane Soares; Silva, Osmar N.; Viana, Juliane Cançado; Porto, William F.; Migliolo, Ludovico; Cunha, Nicolau Brito da; Gomes, Nelson; Fensterseifer, Isabel C. M.; Colgrave, Michelle L.; Craik, David J.; Dias, Simoni Campos; Franco, Octávio L.

doi:10.1021/acs.jnatprod.6b00270

Cited by 27 publications

(26 citation statements)

References 51 publications

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“…A large population of other cyclotides from diverse plant families, ie, cycloviolacin O2, cycloviolacin O3, cycloviolacin O19, CterB, CterE, CterG, CterR, tricyclon A, varv A, kalata B1, kalata B2, kalata B13, kalata B7, vibiG, vibiH, vibiD, vibiE, psyle A, psyle C, psyle E, vaby A, and vaby D, cycloviolacin O4 exhibited cytotoxic activity against lymphoma cell line (U‐937 GTB) . Apart from cyclotides, acyclic peptides also have been reported to show cytotoxic action against cancer cell lines …”

Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

“…Acyclotides such as parigidin‐br3, chassatide C7, C8, and C11 from Rubiaceae, and cliotide T1 and cliotide T3 from Fabaceae, have been reported to be cytotoxic to HeLa cancer cell line.…”

Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

“…33 More recently, a number of cyclotides from Viola ignobilis including vigno 5, an acyclotide has been proven to induce apoptosis dependent cytotoxicity against HeLa cell lines. 34 Acyclotides such as parigidin-br3, 42 chassatide C7, C8, and C11 43 from Rubiaceae, and cliotide T1 and cliotide T3 from Fabaceae, 44 have been reported to be cytotoxic to HeLa cancer cell line.…”

Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

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Natural and grafted cyclotides in cancer therapy: An insight

Mehta

Dhankhar

Gulati

et al. 2020

Journal of Peptide Science

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Cyclotides is a rapidly growing class of plant‐derived cyclic peptides exhibiting several bioactivities with potential applications in the agricultural and pharmaceutical sectors. Both natural and grafted cyclotides have shown promise in cancer therapy. Approximately 70 natural cyclotides belonging to three plant families (Fabaceae, Rubiaceae, and Violaceae) have shown cytotoxicity against several cancer cell lines. Cyclotides exhibit considerable stability against thermal and enzymatic proteolysis, owing to their unique structure with knotted topology and head to tail cyclization. Further, their small size, high stability, oral bioavailability, and tolerance to amino acid substitution in structural loops make them an ideal platform for designing peptide‐based drugs for cancer. Thus, cyclotides provide ideal scaffolds for bioactive epitope grafting and facilitating drug delivery in cancer treatment. Many anticancer linear peptides have been grafted in cysteine knotted cyclic framework of cyclotide for enhancing their cell permeability across cellular membranes, thereby improving their delivery and pharmacokinetics. The present review comprehensively discusses the distribution, toxicity, and anticancer bioactivity of natural cyclotides. Further, it systematically elaborates on the role and action of epitopes' into grafted cyclotides in targeting cancer. The review also encompasses related patents landscape study and future challenges in peptide‐based cancer therapy.

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Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

“…Acyclotides such as parigidin‐br3, chassatide C7, C8, and C11 from Rubiaceae, and cliotide T1 and cliotide T3 from Fabaceae, have been reported to be cytotoxic to HeLa cancer cell line.…”

Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

Section: Natural Cyclotides For Cancer Therapymentioning

confidence: 99%

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Natural and grafted cyclotides in cancer therapy: An insight

Mehta

Dhankhar

Gulati

et al. 2020

Journal of Peptide Science

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“…Similar cytotoxic activities have been also reported in vitro against MCF-7 (breast cracinoma) and Caco-2 (colorectal adenocarcinoma) cells with acyclic cyclotides isolated from the plant Palicourea rigida . [77] …”

Section: Biological Activities Of Naturally-occurring Cyclotidesmentioning

confidence: 99%

Cyclotides: Overview and Biotechnological Applications

Gould

Camarero

2017

ChemBioChem

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Cyclotides are globular microproteins with a unique head-to-tail cyclized backbone, stabilized by three disulfide bonds forming a cystine knot. This unique circular backbone topology and knotted arrangement of three disulfide bonds makes them exceptionally stable to chemical, thermal and biological degradation compared to other peptides of similar size. In addition, cyclotides have been shown to be highly tolerant to sequence variability aside from the conserved residues forming the cystine knot. Cyclotides can also cross cellular membranes and are able to modulate intracellular protein-protein interactions both in vitro and in vivo. All these features make cyclotides appear as highly promising leads or frameworks for the design of peptide-based diagnostic and therapeutic tools. This article provides an overview on cyclotides and their applications as molecular imaging agents and peptide-based therapeutics.

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“…Commonly, AMPs have been identified and studied by traditional purification experiments, screening different targets (Mandal et al., , ; Pinto et al., ). Molecular biology could also be an alternative for this identification (Cunha et al., ; Porto, Miranda, Pinto, Dohms, & Franco, ).…”

Section: Discussionmentioning

confidence: 99%

Structure‐guided identification of antimicrobial peptides in the spathe transcriptome of the non‐model plant, arum lily (Zantedeschia aethiopica)

et al. 2019

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Antimicrobial peptides (AMPs) are small molecules present in all living beings. Despite their huge sequence variability, AMPs present great structural conservation, mainly in cysteine‐stabilized families. Moreover, in non‐model plants, it is possible to detect cysteine‐stabilized AMPs (cs‐AMPs) with different sequences not covered by conventional searches. Here, we described a threading application for cs‐AMP identification in the non‐model arum lily (Zantedeschia aethiopica) plant, exploring the spathe transcriptome. By using the predicted proteins from the Z. aethiopica transcriptome as our primary source of sequences, we have filtered by using structural alignments of 12 putative cs‐AMP sequences. The two unreported sequences were submitted to PCR validation, and ZaLTP7 gene was confirmed. By using the structure alignments, we classified ZaLTP7 as an LTP type 2‐like. The successful threading application for cs‐AMP identification is an important advance in transcriptomic and proteomic data mining. Besides, the same approach could be applied to the use of NGS public data to discover molecules to combat multidrug‐resistant bacteria.

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Characterization of a Bioactive Acyclotide from Palicourea rigida

Cited by 27 publications

References 51 publications

Natural and grafted cyclotides in cancer therapy: An insight

Natural and grafted cyclotides in cancer therapy: An insight

Cyclotides: Overview and Biotechnological Applications

Structure‐guided identification of antimicrobial peptides in the spathe transcriptome of the non‐model plant, arum lily (Zantedeschia aethiopica)

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